Stable isotopes are powerful research tools in environmental sciences and their use in ecosystem research is increasing. Stable isotope measurements allow the study of evapotranspiration fluxes, soil evaporation and leaf transpiration phenomena. Soil water and leaf water are the sources of the evapotranspiration that transfers large quantities of water from land to the atmosphere; as a result the isotopic composition of water left in the leaves is modified towards enrichment. Evaporation also changes the isotopic composition of water bodies creating a natural isotopic signal. The isotopic identity of soil water affects the oxygen isotopic signature of leaf and stem water. In this paper we present the isotopic data of bulk leaf water, showing the enrichment in isotopic value of oxygen due to evapotranspiration from leaves in conjunction with the isotopic signal of rainwater and other environmental factors such as humidity and temperature. Results suggest that the variation in the values of δ18O of Eucalyptus citriodora, Dalbergia sissoo, Melia azedarach and Pinus roxburghii is due to the seasonal changes in the δ18O of the source water for plants, i. e. rain. It is further observed that leaf water δ18O values are depleted during the months of July, August and September. This occurs due to the following reasons: (a) the sampling areas receive about 50% of the average annual rain during these months, and (b) rainfalls during these months are isotopically depleted compared with winter rains. Citation Butt, S., Ali, M., Fazil, M. & Latif, Z. (2010) Seasonal variations in the isotopic composition of leaf and stem water from an arid region of Southeast Asia. Hydrol. Sci. J. 55(5), 844-848. [ABSTRACT FROM AUTHOR]