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Summary: The study was carried out in the northern part of the Aquitaine Basin extending in Southwest France. The basin has been intensively exploited for various purposes for many years. Although the geological context is well known, there are still some gaps in the knowledge about the hydrogeochemical regime, groundwater average residence time and the palaeohydrological conditions prevailing during the recharge period which would help to secure sustainable exploitation of the resources. Environmental isotopes (18O, 2H, 3H, 13C) together with radiocarbon were used to evaluate hydrochemical evolution, residence time and palaeorecharge conditions for Jurassic, Cretaceous and Eocene aquifers. Radiocarbon activity in the aquifers varies widely between 0 and 94pmc. After radiocarbon corrections the ages indicate both modern and old waters sometimes exceeding the limit for 14C dating. The Fontes and Garnier model is best adapted to the specificity of the carbonate system. The content in stable isotopes of water is varying between −7.7‰ and −4.9‰ for δ18O and −52.3‰ to −29.6‰ for δ2H. The wide ranges of stable isotopic values imply variable climatic conditions. A group of isotopically depleted samples was detected, indicating colder climatic conditions during the recharge. Radiocarbon ages calculation, together with isotopic signature, point out that the depletion in stable isotopic values fits the period between 20 and 15ka B.P. and therefore indicate the Last Glacial Maximum (LGM). According to the correction model, the transition from Pleistocene to Holocene would occur between 15 and 12ka B.P. Palaeohydrological data from the Northern Aquitaine Basin does not confirm a significant hiatus in the recharge history. The recharge conditions in South Europe seem to have occurred under discontinuous permafrost conditions as documented by many palaeoclimatological archives. This is in favour of an uninterrupted recharge of the confined aquifers of Northern Aquitaine Basin. Such information should be considered by modellers in their attempts to simulate the hydrogeological functioning of large confined aquifers on the very long time range. [Copyright &y& Elsevier]