Search

Your search keyword '"Baechi, T"' showing total 2 results
Start Over Author "Baechi, T" Topic spleen
2 results on '"Baechi, T"'

1. Evidence for different receptor sites in mouse spleen cells for the Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins.

Author

Peterhans E, Baechi T, and Yewdell J

Subjects
Binding Sites, HN Protein, Liposomes, Luminescent Measurements, Receptors, Virus metabolism, Spleen metabolism, Viral Fusion Proteins, Cell Membrane metabolism, Parainfluenza Virus 1, Human analysis, Spleen cytology, Viral Proteins metabolism
Abstract

Liposomes were reconstituted from phosphatidylcholine and Sendai virus glycoproteins HN or F and their interaction with mouse spleen cells was studied. Both the HN and F liposomes were able to stimulate chemiluminescence (CL), indicating that the glycoproteins were able to interact with the cell membrane independently of each other. The induction of CL in cells which had been pretreated with liposomes by monoclonal antibodies to either HN or F demonstrated that HN and F bind to the cells independently. The presence of F liposomes on the cell surface was also confirmed by immunoelectron microscopy. Cells pretreated with HN and F liposomes revealed a different pattern of CL when challenged with intact virus or the calcium ionophore A23187 indicating that HN and F bind to different receptor sites.

Published
1983
Full Text
View/download PDF

2. Evidence for different receptor sites in mouse spleen cells for the Sendai virus hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins

Author

Yewdell J, Peterhans E, and Baechi T

Subjects
Immunoelectron microscopy, Biology, Cell membrane, Viral Proteins, Virology, medicine, chemistry.chemical_classification, Liposome, Binding Sites, HN Protein, Cell Membrane, biology.organism_classification, Sendai virus, Parainfluenza Virus 1, Human, medicine.anatomical_structure, chemistry, Biochemistry, Cell culture, Liposomes, Luminescent Measurements, biology.protein, Receptors, Virus, Glycoprotein, Neuraminidase, Hemagglutinin-neuraminidase, Viral Fusion Proteins, Spleen
Abstract

Liposomes were reconstituted from phosphatidylcholine and Sendai virus glycoproteins HN or F and their interaction with mouse spleen cells was studied. Both the HN and F liposomes were able to stimulate chemiluminescence (CL), indicating that the glycoproteins were able to interact with the cell membrane independently of each other. The induction of CL in cells which had been pretreated with liposomes by monoclonal antibodies to either HN or F demonstrated that HN and F bind to the cells independently. The presence of F liposomes on the cell surface was also confirmed by immunoelectron microscopy. Cells pretreated with HN and F liposomes revealed a different pattern of CL when challenged with intact virus or the calcium ionophore A23187 indicating that HN and F bind to different receptor sites.

Published
1983

Catalog

Books, media, physical & digital resources
See catalog results