There are a thousand of species of spiders, but a few are of medical importance. Phoneutria genus is one of the most aggressive spiders, popularly known as the “Brazilian wandering spider". Phoneutrism cases can be divided into mild, moderate and severe based on clinical symptomatology. The clinical signs are intense pain, edema, mild erythema, emesis, tachycardia, agitation, hypotension, cardiac arrhythmia, pulmonary edema, seizure, priapism and shock. Cardiac troponin I (cTnI) and creatine phosphokinase MB (CKMB) can be used to measure myocardial damage caused by phoneutrism. The aim of this study is report the use of cardiac biomarkers as tool to identify cardiac damage of phoneutrisms in a dog. A one-year-old, mixed breed, male, eight kilograms dog was brought to the Veterinary Hospital presenting yellowish liquid emesis, muscle tremors and disorientation after being bitten by Phoneutria nigriventer identified by Laboratory of Veterinary Toxicology. There were restless behavior, paresis of pelvic limbs and intense muscular fasciculations, tachycardia (140 bpm), tachypnea, dyspnea, weak arterial pulse, cyanotic mucosa, mild dehydration (< 5%), cardiac arrhythmia, pulmonary crepitation, decubitus with tetraparalysis and hyperventilation. A total absence of response to the postural tests was observed on neurological examination. There were absence of pupillary and palpebral reflexes, bilateral miosis, with the right side being lightly responsive, an increase in muscle tone in both pelvic and thoracic limbs with spastic characteristics, anterior patellar reflex was absent, the panicle reflex was diminished, and the animal was incontinent. Hemogram, biochemical tests, gasometry and urinalysis was performed. In venous blood gas analysis, hypokalemia (K + 2.91 mmol/L) and decrease of ionized calcium (iCa 1.84 mg/dL) were observed. Blood pressure was 160 mmHg. The total CK dosage was 2585U/L (reference value = 84 U/L). The treatment was oxygen therapy, furosemide (4 mg/ kg, SC), tramadol (3 mg/kg, IV), intravenous fluid therapy with ringer-lactate (78 mL/hour) supplemented with calcium gluconate and potassium chloride. Omeprazole (1 mg/kg, IV, SID) was added to cephalothin (30 mg/kg, IV, TID). At electrocardiogram, bradyarrhythmia was detected with atrial extrasystoles. The analysis of the two cardiac markers, cTnI and CK-MB were requested. Both were increased (CK-MB [194 U/L] and cTnI [10.78 ng/mL]. On the second day, the dog was agitated, walked normally and fed on appetite. A new dosage of electrolytes performed was within the reference values. The animal was discharged as soon as the fluid therapy was completed. A week later, the animal returned with all parameters within normality; no change was observed on the second electrocardiogram. Even though the condition was considered severe, the dog had good clinical improvement, possible due to the rapid evaluation, including cardiac alterations of the animal and immediate institution of the therapy support. [ABSTRACT FROM AUTHOR]