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Your search keyword '"MS/MS/MS analysis"' showing total 2 results
Start Over Descriptor "MS/MS/MS analysis" Topic sphingomyelins
2 results on '"MS/MS/MS analysis"'

1. Comprehensive Quantitation Using Two Stable Isotopically Labeled Species and Direct Detection of N-Acyl Moiety of Sphingomyelin.

Author

Hama K, Fujiwara Y, Tabata H, Takahashi H, and Yokoyama K

Subjects
Acetyltransferases genetics, Acetyltransferases metabolism, Acylation, Chromatography, Liquid, Fatty Acid Elongases, HeLa Cells, Humans, Spectrometry, Mass, Electrospray Ionization, Sphingomyelins metabolism, Tandem Mass Spectrometry, Up-Regulation, Sphingomyelins analysis
Abstract

Sphingomyelin (ceramide-phosphocholine, CerPCho) is a common sphingolipid in mammalian cells and is composed of phosphorylcholine and ceramide as polar and hydrophobic components, respectively. In this study, a qualitative liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS/MS) analysis is proposed in which CerPCho structures were assigned based on product ion spectra corresponding to sphingosylphosphorylcholine and N-acyl moieties. From MS/MS/MS analysis of CerPCho, we observed product ion spectra of the N-acyl fatty acids as [RCO 2 ] - ions as well as sphingosylphosphorylcholine. A calibration curve for CerPCho was constructed using two stable isotopically labeled CerPCho species and then used to quantify the CerPCho species in HeLa cells as a proof-of-principle study. The present study proposes an accurate method for quantifying and assigning structures to each CerPCho species in crude biologic samples by LC-ESI-MS/MS/MS analysis.

Published
2017
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2. Comprehensive Quantitation Using Two Stable Isotopically Labeled Species and Direct Detection of N-Acyl Moiety of Sphingomyelin

Author

Hideyo Takahashi, Hidetsugu Tabata, Yuko Fujiwara, Kotaro Hama, and Kazuaki Yokoyama

Subjects
0301 basic medicine, Sphingomyelin, Ceramide, Spectrometry, Mass, Electrospray Ionization, Calibration curve, Fatty Acid Elongases, Acylation, Tandem mass spectrometry, Biochemistry, MS/MS/MS analysis, Ion, 03 medical and health sciences, chemistry.chemical_compound, Liquid chromatography-electrospray ionization-tandem mass spectrometry, Acetyltransferases, Tandem Mass Spectrometry, Methods, Moiety, Humans, Chromatography, 030102 biochemistry & molecular biology, Phosphorylcholine, Organic Chemistry, Cell Biology, Sphingolipid, Sphingomyelins, Up-Regulation, 030104 developmental biology, chemistry, lipids (amino acids, peptides, and proteins), N-Acyl fatty acid, Chromatography, Liquid, HeLa Cells
Abstract

Sphingomyelin (ceramide-phosphocholine, CerPCho) is a common sphingolipid in mammalian cells and is composed of phosphorylcholine and ceramide as polar and hydrophobic components, respectively. In this study, a qualitative liquid chromatography-electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS/MS) analysis is proposed in which CerPCho structures were assigned based on product ion spectra corresponding to sphingosylphosphorylcholine and N-acyl moieties. From MS/MS/MS analysis of CerPCho, we observed product ion spectra of the N-acyl fatty acids as [RCO2]− ions as well as sphingosylphosphorylcholine. A calibration curve for CerPCho was constructed using two stable isotopically labeled CerPCho species and then used to quantify the CerPCho species in HeLa cells as a proof-of-principle study. The present study proposes an accurate method for quantifying and assigning structures to each CerPCho species in crude biologic samples by LC–ESI–MS/MS/MS analysis.

Published
2017

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