1. An unexpected gene cluster for downstream degradation of alkylphenols in Sphingomonas sp. strain TTNP3.
- Author
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Kolvenbach BA, Dobrowinski H, Fousek J, Vlcek C, Schäffer A, Gabriel FL, Kohler HP, and Corvini PF
- Subjects
- Bacterial Proteins genetics, Bacterial Proteins metabolism, Biodegradation, Environmental, Biotechnology, Dioxygenases genetics, Dioxygenases metabolism, Fatty Acids, Unsaturated metabolism, Genes, Bacterial, Molecular Sequence Data, Oxidoreductases genetics, Oxidoreductases metabolism, Oxidoreductases Acting on CH-CH Group Donors genetics, Oxidoreductases Acting on CH-CH Group Donors metabolism, Phenols metabolism, Sequence Analysis, DNA, Sphingomonas genetics, Sphingomonas growth & development, Hydroquinones metabolism, Multigene Family genetics, Sphingomonas enzymology
- Abstract
In silico analysis of nucleotide sequences flanking the recently found hydroquinone dioxygenase in Sphingomonas sp. strain TTNP3 revealed a gene cluster that encodes a hydroquinone catabolic pathway. In addition to the two open-reading frames encoding the recently characterized hydroquinone dioxygenase, the cluster consisted of six open-reading frames. We were able to express the three open-reading frames, hqdC, hqdD, and hqdE, and demonstrated that the three gene products, HqdC, HqdD, and HqdE had 4-hydroxymuconic semialdehyde dehydrogenase, maleylacetate reductase, and intradiol dioxygenase activity, respectively. Surprisingly, the gene cluster showed similarities to functionally related clusters found in members of the β- and γ-proteobacteria rather than to those found in other members of the genus Sphingomonas sensu latu.
- Published
- 2012
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