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Your search keyword '"Peng, Dandan"' showing total 4 results
Start Over Author "Peng, Dandan" Topic spermidine
4 results on '"Peng, Dandan"'

1. Spermidine or spermine pretreatment regulates organic metabolites and ions homeostasis in favor of white clover seed germination against salt toxicity.

Author

Cheng B, Hassan MJ, Peng D, Huang T, Peng Y, and Li Z

Subjects
Spermine pharmacology, Spermine metabolism, Seeds metabolism, Seedlings metabolism, Homeostasis, Water metabolism, Medicago, Spermidine pharmacology, Spermidine metabolism, Germination
Abstract

White clover is widely cultivated as a leguminous forage or ground cover plant worldwide. However, soil salinization decreases its yield and quality. Aims of the present experiment were to elucidate the impact of seed pretreatment with spermidine (Spd) or spermine (Spm) on amylolysis, Na + /K + accumulation, and metabolic homeostasis during germination. Seed was soaked in distilled water (control), Spd or Spm solution and then germinated under optimal or salt stress conditions for 7 days. Results showed that germination vigor, germination percentage, or seed vigour index of seeds pretreatment with Spd increased by 7%, 11%, or 70% when compared with water-pretreated seeds under salt stress, respectively. Germination percentage or seed vigour index of seeds pretreatment with Spm increased by 17% or 78% than water-pretreated seeds under saline condition, respectively. In response to salt stress, accelerated amylolysis via activation of β-amylase activity was induced by Spd or Spm pretreatment. Spd or Spm pretreatment also significantly enhanced accumulation of diverse amino acids, organic acids, sugars, and other metabolites (putrescine, myo-inositol, sorbitol, daidzein etc.) associated with enhanced osmotic adjustment, antioxidant capacity, and energy supply during germination under salt stress. In addition, Spd or Spm pretreatment not only significantly reduced salt-induced K + loss and overaccumulation of Na + , but also improved the ratio of K + to Na + , contributing to Na + and K + balance in seedlings. In response to salt stress, seeds pretreatment with Spd or Spm up-regulated transcription level of NHX2 related to enhancement in compartmentation of Na + from cytoplasm to vacuole, thus reducing Na + toxicity in cytoplasm. Spm priming also uniquely up-regulated transcription levels of SKOR, HKT1, and HAL2 associated with K + and Na  +  homeostasis and decline in cytotoxicity under salt stress., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Masson SAS. All rights reserved.)

Published
2024
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2. NO is involved in spermidine-induced drought tolerance in white clover via activation of antioxidant enzymes and genes.

Author

Peng D, Wang X, Li Z, Zhang Y, Peng Y, Li Y, He X, Zhang X, Ma X, Huang L, and Yan Y

Subjects
Ascorbate Peroxidases metabolism, Catalase metabolism, Cyclohexylamines pharmacology, Electrolytes metabolism, Enzyme Inhibitors pharmacology, Malondialdehyde metabolism, Nitrate Reductase metabolism, Nitric Oxide Synthase metabolism, Peroxidases metabolism, Plant Leaves metabolism, Superoxide Dismutase metabolism, Trifolium enzymology, Antioxidants metabolism, Droughts, Nitric Oxide metabolism, Spermidine metabolism, Stress, Physiological, Trifolium metabolism
Abstract

Nitric oxide (NO), a key signaling molecule, can be induced by polyamines (PAs), which play an important role in improving drought tolerance in plants. This study was to further investigate the role of NO in spermidine (Spd)-induced drought tolerance associated with antioxidant defense in leaves of white clover (Trifolium repens) under drought stress induced by -0.3 MPa polyethylene glycol (PEG-6000) solution. A hydroponic growth method was used for cultivating plants in a controlled growth chamber for 30-33 days until the second leaves were fully expanded. Two relative independent experiments were carried out in our study. One is that exogenous application of Spd or an NO donor (sodium nitroprusside (SNP)) significantly improved drought tolerance in whole plants, as demonstrated by better phenotypic appearance, increased relative water content (RWC), and decreased electrolyte leakage (EL) and malondialdehyde (MDA) content in leaves as compared to untreated plants. For another detached leaf experiment, PEG induced an increase in the generation of NO in cells and significantly improved activities of nitrate reductase (NR) and nitric oxide synthase (NOS). These responses could be blocked by pre-treatment with a Spd biosynthetic inhibitor, dicyclohexyl amine (DCHA), and then reversed by application of exogenous Spd. Meanwhile, PEG induced up-regulation of activities and gene transcript levels of corresponding antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) to varying degrees, while these effects were partially blocked by pre-treatment with DCHA, the scavenger of NO, the inhibitors of NR or NOS. In addition, Spd-induced antioxidant enzyme activities and gene expression also could be effectively inhibited by an NO scavenger as well as inhibitors of NR and NOS. These findings suggest that both Spd and NO can enhance drought tolerance. Spd was involved in drought stress-activated NR and NOS pathways associated with NO release, which mediated antioxidant defense and thus contributed to drought tolerance in white clover.

Published
2016
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3. Polyamine regulates tolerance to water stress in leaves of white clover associated with antioxidant defense and dehydrin genes via involvement in calcium messenger system and hydrogen peroxide signaling

Author

Xiaojuan Wang, Xinquan Zhang, Xiao Ma, Linkai Huang, Yanhong Yan, Yan Zhang, Peng Dandan, Yan Peng, Zhou Li, and Xiaoshuang He

Subjects
antioxidant, dehydrin, Calmodulin, Physiology, Spermine, Plant Science, oxidative damage, lcsh:Physiology, chemistry.chemical_compound, polyamine, Physiology (medical), Original Research, NADPH oxidase, biology, lcsh:QP1-981, Activator (genetics), Correction, regulation, Spermidine, Cytosol, chemistry, Biochemistry, biology.protein, Putrescine, gene expression, Polyamine, white clover (Trifolium repens)
Abstract

Endogenous polyamine (PA) may play a critical role in tolerance to water stress in plants acting as a signaling molecule activator. Water stress caused increases in endogenous PA content in leaves, including putrescine (Put), spermidine (Spd), and spermine (Spm). Exogenous application of Spd could induce the instantaneous H2O2 burst and accumulation of cytosolic free Ca(2+), and activate NADPH oxidase and CDPK gene expression in cells. To a great extent, PA biosynthetic inhibitor reduced the water stress-induced H2O2 accumulation, free cytosolic Ca(2+) release, antioxidant enzyme activities and genes expression leading to aggravate water stress-induced oxidative damage, while these suppressing effects were alleviated by the addition of exogenous Spd, indicating PA was involved in water stress-induced H2O2 and cytosolic free Ca(2+) production as well as stress tolerance. Dehydrin genes (Y 2 SK, Y 2 K, and SK 2) were showed to be highly responsive to exogenous Spd. PA-induced antioxidant defense and dehydrin genes expression could be blocked by the scavenger of H2O2 and the inhibitors of H2O2 generation or Ca(2+) channels blockers, a calmodulin antagonist, as well as the inhibitor of CDPK. These findings suggested that PA regulated tolerance to water stress in white clover associated with antioxidant defenses and dehydrins via involvement in the calcium messenger system and H2O2 signaling pathways. PA-induced H2O2 production required Ca(2+) release, while PA-induced Ca(2+) release was also essential for H2O2 production, suggesting an interaction between PA-induced H2O2 and Ca(2+) signaling.

Published
2016

4. NO is involved in spermidine-induced drought tolerance in white clover via activation of antioxidant enzymes and genes

Author

Yaping Li, Yan Peng, Linkai Huang, Yan Zhang, Xiaoshuang He, Yanhong Yan, Zhou Li, Xiaojuan Wang, Peng Dandan, Xinquan Zhang, and Xiao Ma

Subjects
0106 biological sciences, 0301 basic medicine, Antioxidant, Spermidine, medicine.medical_treatment, Drought tolerance, Plant Science, Nitrate reductase, Nitric Oxide, 01 natural sciences, Nitrate Reductase, Antioxidants, Superoxide dismutase, 03 medical and health sciences, chemistry.chemical_compound, Electrolytes, Ascorbate Peroxidases, Stress, Physiological, Malondialdehyde, medicine, Enzyme Inhibitors, Cyclohexylamines, biology, Superoxide Dismutase, fungi, food and beverages, Cell Biology, General Medicine, APX, Catalase, Droughts, Nitric oxide synthase, Plant Leaves, 030104 developmental biology, chemistry, Biochemistry, Peroxidases, biology.protein, Trifolium, Nitric Oxide Synthase, 010606 plant biology & botany
Abstract

Nitric oxide (NO), a key signaling molecule, can be induced by polyamines (PAs), which play an important role in improving drought tolerance in plants. This study was to further investigate the role of NO in spermidine (Spd)-induced drought tolerance associated with antioxidant defense in leaves of white clover (Trifolium repens) under drought stress induced by −0.3 MPa polyethylene glycol (PEG-6000) solution. A hydroponic growth method was used for cultivating plants in a controlled growth chamber for 30–33 days until the second leaves were fully expanded. Two relative independent experiments were carried out in our study. One is that exogenous application of Spd or an NO donor (sodium nitroprusside (SNP)) significantly improved drought tolerance in whole plants, as demonstrated by better phenotypic appearance, increased relative water content (RWC), and decreased electrolyte leakage (EL) and malondialdehyde (MDA) content in leaves as compared to untreated plants. For another detached leaf experiment, PEG induced an increase in the generation of NO in cells and significantly improved activities of nitrate reductase (NR) and nitric oxide synthase (NOS). These responses could be blocked by pre-treatment with a Spd biosynthetic inhibitor, dicyclohexyl amine (DCHA), and then reversed by application of exogenous Spd. Meanwhile, PEG induced up-regulation of activities and gene transcript levels of corresponding antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) to varying degrees, while these effects were partially blocked by pre-treatment with DCHA, the scavenger of NO, the inhibitors of NR or NOS. In addition, Spd-induced antioxidant enzyme activities and gene expression also could be effectively inhibited by an NO scavenger as well as inhibitors of NR and NOS. These findings suggest that both Spd and NO can enhance drought tolerance. Spd was involved in drought stress-activated NR and NOS pathways associated with NO release, which mediated antioxidant defense and thus contributed to drought tolerance in white clover.

Published
2015

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