1. Human chromatin remodeler cofactor, RNA interactor, eraser and writer sperm RNAs responding to obesity.
- Author
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Swanson GM, Estill M, Diamond MP, Legro RS, Coutifaris C, Barnhart KT, Huang H, Hansen KR, Trussell JC, Coward RM, Zhang H, Goodrich R, and Krawetz SA
- Subjects
- Adult, Body Mass Index, Chromatin Assembly and Disassembly, DNA Methylation, Gene Regulatory Networks, Humans, Male, Obesity metabolism, RNA Processing, Post-Transcriptional, Obesity genetics, Spermatozoa metabolism, Transcriptome
- Abstract
In the United States almost 33% of adults are considered obese (BMI > 30 kg/m
2 ). Both animal models and to a lesser extent human studies, have associated BMI, a measure of obesity, with alterations in sperm DNA methylation and RNAs. Sperm RNAs from the Assessment of Multiple Gestations from Ovarian Stimulation trial, were isolated and sequenced. A Generalized Linear Model identified 487 BMI associated human sperm RNA elements (short exon-sized sequences). They partitioned into four patterns; a continual increase with BMI, increase once obese (BMI>30 kg/m2 ); a steady decrease with BMI; and decrease once overweight (BMI 25 - 30 kg/m2 ). Gene Ontology revealed a unique relationship between BMI and transcripts associated with chromosome organization, adipogenesis, cellular stress and obesity-related inflammation. Coregulatory networks linked by Chromatin remodeler cofactors, RNA interactors, Erasers and Writers (CREWs) were uncovered to reveal a hierarchical epigenetic response pathway.- Published
- 2020
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