1. Chemical structures of the core regions of Campylobacter jejuni serotypes 0:1, 0:4, 0:23, and 0:36 lipopolysaccharides.
- Author
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Aspinall, Gerald O., McDonald, Armando G., Raju, T. Shantha, Pang, Henrianna, Moran, Anthony P., and Penner, John L.
- Subjects
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CAMPYLOBACTER jejuni , *POLYSACCHARIDES , *SIALIC acids , *OLIGOSACCHARIDES , *SPECTRUM analysis , *CHROMATOGRAPHIC analysis - Abstract
Complete structures, including the location of N-acetylneuraminic acid (Neu5Ac) residues, were assigned for the core regions of Campylobacter jejuni serotypes O:1, O:4, and O:23 and O:36 lipopolysaccharides (LPS). In continuation of earlier studies, structure determinations of liberated oligosaccharides and, where necessary, of intact LPS, were by [sup1]H-NMR spectroscopy, Smith degradation, chromium trioxide and enzymic degradations, in conjunction with methylation studies supported by fast-atom-bombardment mass spectrometry and linkage analyses by gas chromatography/mass spectrometry. It was concluded on the basis of the following structures, in which each was linked 1→5 to a terminal 3-deoxy-D-manno-octulosonic acid residue, that the core regions with qualitatively similar sugar compositions showed serotypic differences in one or more of their sequences, linkage types, and anomeric configurations: (formula???).The outer regions of each structure carry Neu5Ac residues linked 2→3 to available β-D-Galp residues and show striking similarities with various glycosphingolipids of the ganglioside family. However, Neu5Ac epitopes are not apparently involved in determining serospecificity. [ABSTRACT FROM AUTHOR]
- Published
- 1993
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