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The Moderate Resolution Imaging Spectroradiometer (MODIS) sensor is currently being operated on both Terra and Aqua spacecrafts. MODIS uses 36 bands arranged in four focal plane assemblies (FPAs)-visible, near infrared, short- and middle-wavelength infrared, and long-wavelength infrared. Misregistrations between spectral bands and FPAs and changes of spatial characterization on-orbit could impact the quality of science data products generated with multiple bands located on different FPAs. In this paper, an approach is presented to compute the MODIS band-to-band registration (BBR) using ground measurements. A special ground scene with unique features is selected to calculate the spatial registration along-scan and along-track. The monthly and yearly spatial deviations are calculated for the bands of both Terra and Aqua MODIS except for some ocean bands, cloud bands, and the Aqua MODIS band 6. The comparison with results derived from the spectroradiometric calibration assembly, a device operated on-orbit to track the BBR shift between any two of the spectral bands, generally shows good agreement. The measured differences between these two approaches are typically less than 100 m in the scan direction and 200 m in the track direction. This approach can provide more frequent characterization of the MODIS BBR and is extremely useful for other sensors that do not have an onboard spatial characterization device. [ABSTRACT FROM AUTHOR]