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Traditional solid phase extraction (SPE) suffers from a lack of specific adsorption. To overcome this problem, a combination of adsorption method and molecular imprinting technology by polydopamine modification was proposed to realize specific recognition of target compounds in SPE, which is of great significance to improve the separation efficiency of SPE. Cellulose hydrogel beads were prepared by dual cross-linking curing method and modified with polydopamine to make them hydrophilic and biocompatible. Subsequently, cellulose hydrogel-based molecularly imprinted beads (MIBs) were synthesized by surface molecular imprinting technology and used as novel column fillers in SPE to achieve efficient adsorption (34.16 mg·g -1 ) with specific selectivity towards camptothecin (CPT) in 120 min. The simulation and NMR analysis revealed that recognition mechanism of MIBs involved hydrogen bond interactions and Van der Waals effect. The MIBs were successful used in separating CPT from Camptotheca acuminata fruits, exhibiting impressive adsorption capacity (1.19 mg·g -1 ) and efficient recovery of CPT (81.54 %). Thus, an environmentally friendly column filler for SPE was developed, offering a promising avenue for utilizing cellulose-based materials in the selective separation of natural products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

In the present study, an efficient ultrasonic/microwave-assisted extraction (UMAE) procedure for the polysaccharides from the fruit of Camptotheca acuminata (CAFP) was investigated and optimized. Under the optimum conditions (ratio of liquid to raw material 30 mL/g, microwave irradiation time of 20 min, microwave irradiation power of 570 W and a fixed ultrasonic power of 50 W obtained by the response surface analysis with Box-Behnken design, satisfactory yields of CAFP (6.81 ± 0.04%) were achieved. The development UMAE technique produced higher yields in a shorter time than conventional hot water extraction (HWE): 20 vs. 120 min. In addition, in vivo CAFP at suitable dose is effective on H22 murine hepatoma strains, and CAFP significantly inhibited the proliferation of human oral carcinoma KB, pancreatic carcinoma BXCP-3 and gastric carcinoma SGC-7901 cells in vitro, indicating CAFP might be suitable for nature antitumor therapeutic agent development., (Copyright © 2018. Published by Elsevier Ltd.)

A reverse micellar microemulsion combined with magnetic solid phase extraction (RMM-MSPE) technology was developed for the extraction and purification of juglone from Juglans mandshurica. Reverse micellar microemulsion (RMM) (Tween 80: n-propanol: n-hexane: 0.05 mol/L KCl aqueous solution = 27:13.5:4.5:55; w/w/w/w) was used to extract juglone based on microwave. The optimum conditions were a pH of 5.6, a liquid-solid ratio of 20:1 (mL/g), temperature of 40 °C, time of 63 s, and power of 400 W. Graphene oxide/nano diamonds/ Fe 3 O 4 (GO/ND/Fe 3 O 4) was prepared as adsorbent and applied to purify juglone by magnetic solid-phase extraction (MSPE). The optimum adsorption conditions were time of 14 h, solid to liquid ratio of 30 mg/mL, pH of 5.5 and shaker speed of 200 r/min. Anhydrous methanol provided the best desorption of juglone and the optimum conditions were a desorption time of 16 h, pH of 8, and the solid-liquid ratio of 40 mg/mL. The purity of juglone was 69.08% and the recovery yield was 78.67%. RMM and GO/ND/Fe 3 O 4 could be reused three times and eight times after the first use, respectively. The proposed RMM-MSPE is a green and efficient alternative method for extraction and purification of juglone. [Display omitted] • MSPE was first applied to the purification of juglone. • Proposal of a green extraction and purification technology: RMM-MSPE. • The RMM solvent and adsorbent, GO/ND/Fe 3 O 4 can be recycled and reused. [ABSTRACT FROM AUTHOR]

[Display omitted] • A novel modified porous cellulose hydrogel based molecularly imprinted bead was proposed. • PCH@MIBs show highly specific ability to separate the targeted alkaloid. • PCH@MIBs are designed as ideal column fillers for solid-phase extraction. • The recognition mechanism was explored using NMR and molecular simulation. 10-hydroxycamptothecine (HCPT), known as a natural alkaloid with significant anti-cancer properties in Camptotheca acuminata , has valuable application prospects in health and medicine and has attracted worldwide attention especially in the field of cancer research. In this study, a porous cellulose hydrogel-based molecularly imprinted bead (PCH@MIBs) with high affinity and selectivity was prepared and served as novel fillers of solid phase extraction (SPE) for selectively separating HCPT. The combination of polydopamine (PDA) modified cellulose hydrogel and highly selective molecular imprinting technology enabled the efficient and targeted adsorption for template molecules. The mixture of nano-calcium carbonate and cellulose solution underwent a reaction with an acidic coagulation bath, which endowed the cellulose hydrogel beads with porous structures. Porous cellulose hydrogel beads were coated with a PDA layer, followed by the preparation of PCH@MIBs using HCPT as template molecules and acrylamide (AM) as functional monomers, resulting in hydrogel-based materials with specific adsorption cavities matched to HCPT. The PCH@MIBs exhibited excellent adsorption capacity (57.87 mg·g−1) and adsorption selectivity (IF = 3.75), as well as good reusability (5 of cycles). The PCH@MIBs were successfully used to separate HCPT from C. acuminata fruits, with an impressive adsorption capacity (0.0974 mg·g−1) and recovery of HCPT (91.27 %). Mechanism analysis demonstrated that the recognition of PCH@MIBs was primarily achieved through non-covalent interactions between AM and HCPT, specifically hydrogen bonds and Van der Waals forces. This work proposes a novel strategy for constructing porous cellulose-based molecularly imprinted hydrogel beads to specific capture targeted compound, which have broad potential for practical application. [ABSTRACT FROM AUTHOR]

• MSPE/BC breaks through limitation that MSPE is only suit for trace enrichment. • A novel separation and purification technology, BE-MSPE/BC was developed. • BE-MSPE/BC is a green system which is easy for large-scale production. Juglone was first preliminary purified by back extraction (BE) which also completed the sustainability of microemulsion as a solvent for extraction of juglone in J. mandshurica Maxim. branches. Then, magnetic solid phase extraction (MSPE) based on bubble column (BC) was proposed to further purify the juglone, GO/ND/Fe 3 O 4 as adsorbent, which broke through the limitation that MSPE is only suitable for trace enrichment. Thus, a BE combined with MSPE based on BC (BE-MSPE/BC) technology to separate and purify juglone from J. mandshurica Maxim. was developed. Under the optimal conditions (optimal adsorption conditions: 0.5 h of adsorption time, 30 mg/mL of solid-liquid ratio, 5.5 of pH, 60 L/min of air flow; optimal desorption conditions: 0.4 h of desorption time, methanol of desorption agent, 100% of desorbent volume fraction, 8 of pH, 40 mg/mL of solid-liquid ratio, 80 L/min of air flow rate), the recovery yield and purity of juglone obtained by BE-MSPE/BC were 80.57% and 72.38%, respectively. BE-MSPE/BC, as a green separation and purification technology that can easily achieve large-scale and sustainable production, is expected to be an alternative method to separate and purify active components in biomass materials and products. [Display omitted] [ABSTRACT FROM AUTHOR]