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Previous studies have focused on total cadmium (Cd) accumulation in rice or its transformation in soil, but only a few have examined the entire soil-rice-human system. This study investigated the Cd bioaccessibility and bioavailability for humans from grains of early rice cultivars grown in a Cd-polluted field and further combined with multi-traits to discover and evaluate the optimum safe production and phytoremediation potential cultivars. The results revealed that Cd concentration in polished rice was0.20 mg kg

Sustainable strategies are essential for zinc (Zn) biofortification and cadmium (Cd) reduction in staple food crops. Herein, we evaluated the phytotoxicity of Glyzinc under foliar and root application (FARA) in a lab-scale experiment, and then investigated its Zn efficiency and Cd reduction through foliar application on wheat (Triticum aestivum L.) under field conditions. Compared to RA, FA of Glyzinc exhibited no adverse effect on wheat growth and oxidative stresses at all doses. In field conditions, FA of Glyzinc remarkably increased Zn (28.7 %), S (10.4 %), Cu (17.3 %) and crude protein (9.1 %) content in wheat grain at 100 mg/L without damaging wheat yield. Furthermore, FA of Glyzinc significantly reduced the grain phytic acid (PA) (23.7 %) and Cd level (19.5 %), as well as PA to Zn molar ratio (32.3 %). Overall, our results indicate that Glyzinc has great potential as a high-efficiency foliar fertilizer for Zn biofortification and safe crop production in nano-enabled agriculture.

Hyperaccumulating ecotypes of Sedum plants are promising Cd/Zn phytoextractors, with potential for leveraging its rhizospheric or endophytic microbiomes to improve phytoremediation efficiency. However, research of bacteria associated with Sedum at field scale is still lacking. Here, we presented a detailed investigation of the bacterial microbiome of hyperaccumulating Sedum ecotypes (S. alfredii and S. plumbizincicola) and a non-hyperaccumulating S. alfredii ecotype, which grow at different soil environments. Moreover, we evaluated the heavy metal uptake and translocation potential of Sedum plants at different locations. The results showed that both HE ecotypes, contrary to the NHE, were efficient for phytoremediation in mine areas and farmlands. For NHE plants, rhizosphere co-occurrence networks were more complex than the networks of other compartments, while networks of HE plants were more complex in bulk soil and roots. The proportion of positive correlations within co-occurrence networks was higher for the HE plants, suggesting a greater potential for mutualistic interactions. Plant compartment and location predominantly shaped the microbiome assembly, and Proteobacteria, Actinobacteria and Acidobacteria dominated the bacterial communities of Sedum plants. Keystone taxa related to Zn hyperaccumulation are similar to those related to Cd hyperaccumulation, and nine bacterial genera had significantly positive correlation with Cd/Zn hyperaccumulation. Taxa, linked to phytoremediation in both mine and farmland (i.e. Actinospica and Streptomyces from Actinobacteria), could be targets for further investigation of their ability to promote metal phytoremediation of Sedum species.

In this study, Rhizobium rhizogenes-mediated root proliferation system in Sedum alfredii has been established. Twenty strains of R. rhizogenes were screened for root proliferation. A significant difference (P0.01) was observed in plant morphological characters under influence of different bacterial strains. The highest root fresh weight (3.236 g/plant) was observed with strain AS12556. Furthermore, significant difference (P0.05) was observed in the chemical composition of organic acids, Tartaric acid (TA), Succinic acid (SA), Malic acid (MA), Citric acid (CA) and Oxalic acid (OA), pH, Total Nitrogen (TN), Total Organic Carbon (TOC) and soluble sugars in root exudates with different R. rhizogenes mediated roots. Furthermore, a series of hydroponics experiments were conducted with varying concentrations of Cd (25, 50 and 75 µM) and Zn (100, 200 and 500 µM) to assess the phytoextraction efficiency of proliferated roots with Rhizobium. Several plants with proliferated roots showed enhanced growth and improved metal extraction efficiency. Five strains (LBA 9402, K599, AS12556, MSU440 and C58C1) were identified as potential strains for root proliferation in Sedum alfredii. R. rhizogenes strain AS12556 improved Cd/Zn phytoextraction by exogenous production of phytochemicals to promote root proliferation, improved shoot biomass, lowered oxidative damage and enhanced phytoextraction efficiency in S. alfredii. Therefore, it has been selected as a potential microbial partner of S. alfredii to develop extensive rooting system for better growth and enhanced phytoremediation potential. Results suggest that R. rhizogenes mediated root proliferation system can be used for optimizing metal extraction from contaminated soils.

Straw residues, as one of the conservation farming practices, are being strongly encouraged in China, including some cadmium-polluted areas. Nowadays, a portion of this plant residue is promoted to be removed for reducing excess metal(loid) in the soil and to be used for bioenergy production. Nevertheless, the possible influences of contaminated straw or the burial of its derived biochars on Cd accumulation in soil and data based on health risk assessment associated with different status and extent of soil contamination were relatively unknown. Thus it is important to provide a more systematical understanding of contaminated straw burial at specific contamination zones, which may provide useful guidance for straw utilization. In this study, we harvested two genotypes of rice straw from 6 contaminated levels among three soil types to comprehensively study the total Cd contents in straws and its derived biochars and correlate the sets of straw characteristics and Cd contents in three different contamination zones. The total Cd concentration in straws grew at a steady rate relatively with increasing soil Cd contamination levels, compared to those in biochars which performed more fluctuate due to the strong burning. According to correlational analyses, three-way ANOVA showed that the moisture, ash, volatile and fixed carbon were all significantly affected by straw CdTotal contents (p

Sedum alfredii Hance is a cadmium (Cd)/zinc (Zn) hyperaccumulator native to China. However, its relatively low biomass restricted the large-scale application for heavy metal contamination remediation. The chromosome set doubling of S. alfredii in vitro was achieved by 0.1%-0.2% (W/V) colchicine treatment. The plant DNA ploidy was analyzed by flow cytometry and chromosome set doubling plants (CSD) were identified based on the obvious different sharp peak. A tissue culture experiment with different Cd treated levels and a field trial with natural polluted mined soil were conducted to study the effects of chromosome doubling on plant biomass and Cd accumulation in shoots. The results suggested that S. alfredii is a mixoploid. Compared with the wild type plants (WT), CSD exhibited typical "gigas" characteristics in morphology including stem thickness, root hair production, number of leaves and size of stoma guard cell. Fresh weight and dry weight of CSD were increased to 1.62-2.03-fold and 2.26-3.25-fold of WT. And Cd content of CSD showed a 17.49%-42.82% increase and 59% increase under tissue culture and field condition, accordingly. In addition, the TF and in BCF of CSD were 2.37- and 1.59-fold of WT, respectively. These results proved that it is feasible to promote phytoextraction efficiency of S. alfredii in Cd contaminated soils through chromosomal engineering, which provides a novel approach for hyperaccumulator application in phytoremediation.

Plant growth promoting bacteria (PGPB) have been reported to have the ability to promote plant growth, development and increase heavy metals (HMs) uptake. Therefore, PGPB inoculation as soil remediation agents into plants with larger biomass and potential of phytoextraction is of great importance to increase bioremediation efficiency. In this study, 12 PGPB strains isolated from a cadmium (Cd)/zinc hyperaccumulator Sedum alfredii Hance were inoculated into non-host plant Brassica juncea and their effects on plant growth and Cd uptake were determined. The results showed that inoculation of most PGPB strains promoted plant growth, boosted root development and improved chlorophyll content in the absence of Cd. Inoculation of PGPB strains promoted plant growth up to 111% in shoot and 358% in root when treated with 2 μM Cd. In addition, PGPB inoculation not only ameliorated plant root morphology including the total root length (RL), total surface area (SA), total root volume (RV) and number of root tips (RT), but also facilitated Cd uptake up to 126%. Furthermore, inoculation of PGPB strains promoted plant Cd accumulation up to 261% in shoot and up to 8.93-fold increase in root. Among all the 12 PGPB strains, Burkholdria SaMR10 and Sphingomonas SaMR12 were identified as the promising microbes for improving phytoremediation efficiency of Cd contaminated soils. These results not only provided useful findings for further investigation of interacting mechanisms between different bacterial strains and plants, but also facilitated the development of microbe-assisted phytoremediation application for HM contaminated soil.

Tetracycline (TC) can enter the human body via the soil-vegetable-human food chain; therefore, it is necessary to understand the toxicity of TC to humans through vegetables grown on contaminated soils. The present study combined an enzyme-linked immunosorbent assay method and an HL-7702 cell model and assessed the bioavailability and toxicity of TC from pak choi (Brassica campestris L. ssp. chinensis) grown on TC-contaminated soils. The results showed that the degradation rate of TC in black soil was significantly higher than that in purplish clay, while the results for TC uptake in pak choi were opposite. The bioaccessibility of TC was found to be higher in pak choi grown on purplish clay (5.67–7.59%) than in that grown on black soil (5.22–6.77%). It is suggested that soil properties contribute to the uptake of TC by pak choi. More fertile soil contained lower TC concentrations and thus mediated lower TC toxicity to humans. It may seem comforting that TC concentrations in the edible parts of pak choi are often found to be below safe limits. However, the TC diagnosis method showed that even moderate increases in TC concentrations in pak choi may induce oxidative stress, liver injury, mitochondrial cristae and rough endoplasmic reticulum swelling, and early apoptosis in liver cells HL-7702. The pak choi grown in purplish clay showed higher TC cytotoxicity than that grown in black soil. The TC cytotoxicity of raw pak choi was found to be higher than that of cooked pak choi. These results provide direct evidence of effective ways to prevent TC toxicity in humans.

Heavy metals contamination in agricultural soil has become a worldwide problem, and soil characteristics modulate metal availability in soils. Four field experiments were conducted simultaneously to evaluate concentration and distribution of cadmium (Cd) and lead (Pb) in 39 oilseed rape cultivars at four agricultural locations with different contamination levels of Cd and Pb, as well as the influence of soil characteristics together with soil total and bioavailable Cd and Pb concentration on metal transfer from soil to oilseed rape. Shoot concentrations of Cd and Pb in oilseed rape cultivars ranged from 0.09 to 3.18 and from 0.01 to 10.5 mg kg−1 across four sites. For most cultivars, Cd concentration in root or shoot were higher than pod and lowest in seed, while the highest Pb concentration was observed in root followed by shoot and seed. Stepwise multiple linear regression analysis allows for a better estimation of Cd and Pb concentration in oilseed rape while taking soil properties into consideration. The results demonstrated that Cd and Pb concentration in oilseed rape were correlated with soil organic matter (OM), cation exchange capacity (CEC), available phosphorus (AP), available potassium (AK), sand, soil total and available Cd and Pb concentration, and R2 varied from 0.993 to 0.999 (P

Cadmium contamination in soil, water and food has become a global problem since last century's industrial and agricultural revolution. It is a highly toxic metal with serious consequences on human and animal health. Different natural and anthropogenic sources are responsible for Cd release in the soil which ultimately leads to the food chain. Cd persists in soil for long durations due to its minimal microbial or chemical loss. There are various physical, chemical or biological techniques which are helpful to minimize Cd risk in food chain. Among them, in-situ immobilization with organic, inorganic or clay amendments is a cost-effective and an environment friendly strategy to remediate Cd polluted sites. Lime, biochar, organic wastes, phosphorus fertilizers, sepiolite, zeolite, hydroxyapatite and bentonite are commonly used amendments for amelioration of Cd contaminated soils. These amendments reduce Cd uptake and enhance immobilization by adsorption, complexation, and precipitation processes. This review is aimed to provide a comprehensive note on Cd toxicity in humans and environment, its immobilization by different agents through variety of processes, and comparison of technologies for Cd removal from contaminated sites.

The identification of high yield genotypes that are capable of accumulating multiple heavy metals in the non-edible parts (roots and shoots), but not in the edible parts (seeds) and have desired nutritional value is necessary for accomplishing phytoremediation coupled with agro-production. In this study, 17 fava bean genotypes were screened in two different field conditions to examine their phytoremediation potential in terms of uptake and translocation of Cd and Pb. Ten genotypes, LBAO, JNJX, DCAN, QXCJ, QIKM, LXYC, YDL6, RBCD, QPID and ZHW6 were found as the best accumulators for Cd and Pb with permissible limit of metals in seeds. The concentration of plant nutrients were genotype and soil type dependent and there was a significant correlation between these two factors. Furthermore, the three genotypes DCAN, LBAO and LXYC showed best performance in alluvial soil type while QPID, RBCD and LXYC were the best in red soil type. Genotype LXYC was similar for both soil types and appeared to be the best fit for phytoremediation coupled with agro-production for slightly or moderately Cd and Pb co-contaminated soil. Therefore, fava bean LXYC genotype is suggested as a potential candidate for phytoremediation of Cd/Pb co-contaminated soils coupled with agro-production.

Climate-related flooding poses a potential challenge to phytoremediation of metal polluted areas. In the present study, uptake, translocation and accumulation of heavy metals (Cd, Zn and Cu), and their phytoavailability in six flood-tolerant Salix genotypes were investigated under simulated seasonal flooding conditions (non-flooding conditions were kept as the control). Plants were cultivated in a greenhouse with open windows using the soil polluted with Cd, Zn and Cu for 110 days. All the control (non-flooded) genotypes did not exhibit visible toxic symptoms, whereas the flooded genotypes showed leaf chlorosis and developed both lenticels and adventitious roots. Biomass production and metal accumulation in tissues varied with Salix genotypes. The flooded genotypes dramatically decreased aerial biomass production compared with corresponding non-flooded genotypes. All the control Salix genotypes showed relatively high accumulation for Cd, Zn and Cu in aerial parts due to high EDTA-extractable metals in the rhizosphere, exhibiting phytoextraction features. In contrast, the flooded genotypes drastically decreased uptake, translocation, accumulation, and extraction capacities for Cd, Zn and Cu in aerial parts, differing with genotypes, and tended to phyto-stabilize them in roots, especially Cu. This study indicated that flooding is a leading factor on phytoremediation efficiency for contaminated sites with willows.

Soil trace elements (TEs) contamination has become a worldwide problem in arable lands and poses great risk to human health via food chain. Intercropping of hyperaccumulator and cash crops is now proposed as a promising alternative phytoremediation technique to address the issue. However, the effect of intercropping in different soil types and field-scale benefits evaluation are rarely reported. A greenhouse pot experiment and a field trial were therefore designed to explore the effects of intercropping Sedum alfredii (hyperaccumulative population) and oilseed rape on Cd phytoextraction potential, Cd transport and crop production, as well as establishing a feasible assessment framework on the basis of benefits evaluation in contaminated soils. Compared with oilseed rape monoculture, intercropping with S. alfredii significantly and consistently increased biomass, seed yield and Cd accumulation in oilseed rape in five typical soil types. Accumulations of Cd varied with soil types, ranging from 22.8 to 4000 μg pot−1. Stepwise multiple linear regression analysis (SMLRA) showed Cd concentrations in plants were related to available phosphorus (AP), pH, soil organic matter (OM), available potassium (AK), silt and sand; R2 values varied from 0.834 to 0.994 (P

Cadmium (Cd) pollution in cultivated soils has posed a great risk to human health through the soil-plant-human pathway. Therefore, it is important to derive soil thresholds for the low-Cd accumulating genotype of wheat (Triticum aestivum L.) to promote its application in agricultural production on Cd-contaminated sites. Here, a pot experiment was performed to explore the transfer characteristics of Cd in two contrasting wheat genotypes at three different soils and the effect of soil parameters together with soil safety Cd thresholds derivation. Generally, grain Cd highly accumulating wheat genotype (Zhenmai10, HT) showed higher Cd accumulation in grains than grain Cd weakly accumulating wheat genotype (Aikang58, LT). Stepwise multiple linear regression (SMLR) analysis (log-transformed Freundlich-type) indicated that Cd accumulation in wheat grains was strongly related to soil total Cd concentration and pH for both genotypes (R2 = 0.907*** for HT; R2 = 0.910*** for LT). Combining the simple regression model of soil-plant transfer system with the risk assessment method based on human health, soil total Cd thresholds for three soils were calculated with the values of 0.62, 0.82, and 0.62 mg kg-1 in LT genotype and 0.31, 0.77, and 0.49 mg kg-1 in HT genotype. Therefore, we suggested that when deducing soil thresholds, the ability of wheat genotypes to accumulate Cd and soil properties should be considered because of the large differences in soil thresholds between different genotypes and types of soils. We believe our results will promote the application of low-Cd wheat genotypes to agricultural production, thereby ensuring the safety of their products.

To reduce cadmium (Cd) pollution of food chains, screening and breeding of low-Cd-accumulating genotypes have received increasing attention. However, the mechanisms involving Cd tolerance and accumulation are not fully understood. Here, we investigated the physiological responses and metabolomics profiling on two wheat (Triticum aestivum L.) genotypes, a low-Cd-accumulating genotype in grains (Aikang58, AK58) and a high-Cd-accumulating genotype in grains (Zhenmai10, ZM10), in hydroponic culture treated without/with Cd for 7 days. The results showed that AK58 was a Cd tolerant genotype with higher capacity of antioxidant systems in root. In addition, the concentrations of Cd bound to root cell walls were higher in AK58 than ZM10, of which pectin and hemicellulose played important roles in Cd binding. Moreover, subcellular distribution manifested that Cd sequestrated in the vacuoles was another tolerance mechanism in AK58. Simultaneously, metabolomics profiling showed that, in AK58, phenylalanine metabolism, alanine, aspartate and glutamate metabolism, isoquinoline alkaloid biosynthesis, arginine and proline metabolism, arginine biosynthesis and glyoxylate and dicarboxylate metabolism are highly related to antioxidant defense system, cell wall biosynthesis and metabolisms of phytochelatins together with other organic ligands, playing crucial roles in Cd tolerance and Cd fixation mechanisms in roots. These novel findings should be useful for molecular assisted screening and breeding of low Cd-accumulating genotypes for wheat crop.

Plant growth-promoting bacteria (PGPB) can promote root uptake and shoot accumulation of cadmium (Cd) in hyperaccumulator plants, but the mechanisms by which PGPB accelerate root-to-shoot transport of Cd is still unknown. A better understanding of these mechanisms is necessary to develop the strategies that can promote the practical phytoextraction of Cd-polluted soils. In this study, we found that Pseudomonas fluorescens accelerates a reversed and a long-distance transport of Cd and sucrose in Sedum alfredii, by examining the xylem and phloem sap and by quantifying the concentrations of Cd and sucrose in shoot and root. The transcriptome sequencing has revealed the up-regulated expressions of starch metabolism and sucrose biosynthesis related genes in the shoots of Cd hyperaccumulator plant S. alfredii that was inoculated with PGPB P. fluorescens. In addition, the genes of sugar, cation and anion transporters were also up-regulated by bacterial treatment, showing a complicated co-expression network with sucrose biosynthesis related genes. The expression levels of Cd transporter genes, such as ZIP1, ZIP2, HMA2, HMA3 and CAX2, were elevated after PGPB inoculation. As a result, the PGPB successfully colonized the root, and promoted the sucrose shoot-to-root transport and Cd root-to-shoot transport in S. alfredii. Since non-photosynthetic root-associated bacteria usually obtain sugars from photosynthetic plants, our results highlight the importance of PGPB-induced changes in hyperaccumlator plants for both the host and the PGPB.

Improving biomass enzymatic saccharification is effective for crop straw utilization, whereas phytoremediation is efficient for trace metal elimination from polluted agricultural soil. Here, we found that the green proteins extracted from Amaranthus leaf tissue could act as active biosurfactant to remarkably enhance lignocellulose enzymatic saccharification for high bioethanol production examined in eight grassy and woody plants after mild chemical and green-like pretreatments were performed. Notably, this study estimated that total green proteins supply collected from one-hectare-land Amaranth plants could even lead to additional 6400-12,400 tons of bioethanol, being over 10-fold bioethanol yield higher than those of soybean seed proteins and chemical surfactant. Meanwhile, the Amaranth green proteins were characterized as a dominated biosorbent for multiple trace metals (Cd, Pb, As) adsorption, being 2.9-6 folds higher than those of its lignocellulose. The Amaranth plants were also assessed to accumulate much more trace metals than all other plants as previously examined from large-scale contaminated soils. Furthermore, the Amaranth green proteins not only effectively block lignin to release active cellulases for the mostly enhanced biomass hydrolyzes, but also efficiently involve in multiple chemical bindings with Cd, which should thus address critical issues of high-costly biomass waste utilization and low-efficient trace metal remediation.

Information is needed for comparative assessment and agronomic practices for phytoavoidation in multi-pollutant field. A field study was conducted to explore 97

Phytoremediation is perceived as a promising technique for remediation of heavy metal (HM) contaminated soils, while the harvested HM-enriched hyperaccumulator biomass should be appropriately disposed. Recently, various thermal treatments of hyperaccumulator have drawn increasing attention. After thermal treatment, the hyperaccumulator was converted to bio-oil, bio-gas, biochar, or ash in accordance with the corresponding conditions, and the HMs were separated, immobilized, or trapped. The migration and transformation of HMs during the thermochemical conversion processes are critical for the safe disposal and further utilization of HM hyperaccumulator. This paper provides a systematic review on the migration and transformation of typical HMs (Cd, Ni, Mn, As, and Zn) in hyperaccumulator during various thermochemical conversion processes, and special emphasis is given to the production and application of targeted products (e.g. biochar, hydrochar, bio-oil, and syngas). Besides, future challenges and perspectives in the thermal treatment of hyperaccumulator are presented as well. The distribution and speciation of HMs were influenced by thermal technique type and reaction conditions, thereby affecting the utilization of the derived products. This review suggests that speciation and availability of HMs in hyperaccumulator are tunable by varying treatment techniques and reaction conditions. This information should be useful for the selective conversion of hyperaccumulator into green and valuable products.

Fluorosis is a chronic systemic disease induced by excessive intake of fluoride (F−). Fluoride in water and foods has been widely concerned, while limited reports focused on F− in soils and tobacco leaves which could transfer to human body. In the present study, we mainly focused on the distribution of F− in tobacco-planted soils and tobacco leaves in Bijie City, Southwest China. Soil total F− concentration ranged from 443.7 to 5,979 mg kg−1. The level of F− extracted by water (FH2O) and KCl solution (FKCl) ranged from 0.58 to 25.55 mg kg−1 and from 0.67 to 21.35 mg kg−1, respectively; hence, FH2O could be used to indicate the bioavailability of soil F− in the study area. The sequential extraction of F− show that the residual and exchangeable F− was the highest (97.44–99.73% of the total F−) and lowest (less than 0.25%) fractions of collected soil samples, respectively. According to the distribution of total and soluble F− in the soil profiles at the depth of 0–100 cm, soils were polluted mainly at the 0–40 cm layer. The soluble F− content in rhizosphere soils were higher than that in bulk soils, and tobacco leaves accumulated F− ranged from 16.73 to 111.3 mg kg−1 which was affected by soil pH and Ca content. Tobacco leaves F− level was related to the maturity of the leaves, with the F−content of medium leaves being higher than that of top leaves. More attention should be paid to tobacco with high F− content since F− pollution may transfer to human body via tobacco smoking.

Cadmium (Cd) is a toxic trace metal that has polluted 20% of agricultural land in China where its concentration exceeds the standards for Chinese farmland. Plants are capable of accumulating Cd and other trace metals, but this capacity varies with species and cultivars within a species. Rice is a staple food consumed by half of the global population. In order to select safe late rice cultivars that are suitable late rice cultivars that can be cultivated in for growing in slightly contaminated soil, a two-year field experiment was conducted with 27 in the first year and 9 late rice cultivars in the second year. The results showed that plant Cd concentrations varied among the cultivars, with high magnitudes of variation occurred in straw and grains. Five genotypes including LR-12, LR-17, LR-24, LR-25 and LR-26 were identified as low accumulators for the first year while LR-15 and LR-17 were identified as promising cultivars based on Cd concentration in the polished rice grains (&lt, 0.02 mg kg&minus, 1 DW). In addition, these cultivars had favorable traits, including mineral nutrition and grain yield. Therefore, these genotypes should be considered for cultivation in slightly or moderately Cd contaminated soils.

Phytoremediation coupled with agro-production is considered a sustainable strategy for remediation of trace element contaminated fields without interrupting crop production. In this study hyperaccumulator Sedum alfredii was intercropped with a leguminous plant fava bean (Vicia fava) in cadmium (Cd) and lead (Pb) co-contaminated field to evaluate the effects of intercropping on growth performance and accumulations of trace elements in plants with plant growth promoting endophyte (PGPE) consortium application. The results showed, compared with monoculture, intercropping coupled with inoculation application promoted biomass as well as Cd and Pb concentrations in individual parts of both plants, thus increasing the removal efficiencies of trace elements (4.49-folds for Cd and 5.41-folds for Pb). Meanwhile, this superposition biofortification measure maintained normal yield and nutrient content, and limited the concentration of Cd and Pb within the permissible limit (

Background Microbes isolated from hyperaccumulating plants have been reported to be effective in achieving higher phytoextraction efficiency. The plant growth-promoting bacteria (PGPB) SaMR12 from the cadmium (Cd)/zinc hyperaccumulator Sedum alfredii Hance could promote the growth of a non-host plant, oilseed rape, under Cd stress. However, the effect of SaMR12 on Brasscia juncea antioxidative response under Cd exposure was still unclear. Results A hydroponic experiment was conducted to study the effects of Sphingomonas SaMR12 on its non-host plant Brassica juncea (L.) Czern. under four different Cd treatments. The results showed that SaMR12 could colonize and aggregate in the roots and then move to the shoots. SaMR12 inoculation promoted plant growth by up to 71% in aboveground biomass and 81% in root biomass over that of the non-inoculated plants. SaMR12-inoculated plants significantly enhanced root Cd accumulation in the 10 and 20 μM Cd treatments, with 1.72- and 0.86-fold increases, respectively, over that of the non-inoculated plants. SaMR12 inoculation not only decreased shoot hydrogen peroxide (H2O2) content by up to 38% and malondialdehyde (MDA) content by up to 60% but also reduced proline content by 7–30% in shoots and 17–32% in roots compared to the levels in non-inoculated plants. Additionally, SaMR12 inoculation promoted the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) and facilitated the relative gene expression levels of dehydroascorbate reductase (DHAR) and glutathione reductase (GR) involved in the glutathione (GSH)-ascorbic acid (AsA) cycle. Conclusions The results demonstrated that, under Cd stress, SaMR12 inoculation could activate the antioxidative response of B. juncea by decreasing the concentrations of H2O2, MDA and proline, increasing the activities of antioxidative enzymes, and regulating the GSH-AsA cycle. These results provide a theoretical foundation for the potential application of hyperaccumulator endophytic bacteria as remediating agents to improve heavy metal tolerance within non-host plant species, which could further improve phytoextraction efficiency. Graphical abstract

Soil pollution with heavy metals has become a common problem in agricultural ecosystems and poses a threat to food safety and human health. Intercropping is now considered a promising alternative to address this issue. However, our understandings about the influences of intercropping systems on rhizosphere microbiota composition and their association with plant performance are still limited. In this study, rhizobox microcosm experiments were conducted to investigate the influence of cropping regimes (i.e. monoculture and intercropping) on the rhizosphere bacterial microbiota and their linkages with the phytoextraction of cadmium (Cd) by Zhongyouza 19 (Brassica napus L.), Xikou Huazi (Brassica juncea L.) and Sedum alfredii using 16S rRNA gene sequencing. Cadmium accumulation in shoots of B. napus and B. juncea grown under intercropping were enhanced by 370% and 27.8% respectively, as compared to monoculture. Soil compartmentation as a major determinant explained 57.6% of the rhizosphere bacterial microbiota variation, whereas plant species and cropping regime accounted for 26.4% of the variation. The overall abundance of the taxa was Proteobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, Verrucomicrobia, and Actinobacteria. Intercropping significantly enriched amplicon sequence variants (ASVs) abundance belonging to Actinobacteria, Bacilli, Deltaproteobacteria while depleting that of Acidobacteria in rhizosphere. Intercropping with S. alfredii influenced more on microbial composition of B. napus rhizosphere. The change in rhizosphere bacterial communities was related to metal availability, soil properties, and plant parameters. The enriched families of Pedosphaeraceae, Ruminococcaceae, Chitinophagaceae, Gemmatimonadaceae, Nitrosomonadaceae, and Parachlamydiaceae were positively correlated with metal concentration in plants. These results indicate that S. alfredii and oilseed rape intercropping could be a promising approach for enhancing the remediation of Cd contaminated soil. Understanding the complex plant-microbe-metal interactions of intercropping system could facilitate the development of remediation strategy for phytoremediation of contaminated soils and sustainable agricultural production.

The willow (

In the present study, the viability of using manure (M), lime (L), and sepiolite (S) alone and in combinations (M/L, M/S, and M/L/S) was evaluated for the remediation of a red paddy soil artificially contaminated with three levels of cadmium (Cd- 0.6, 1, and 2 mg kg-1 soil). Experiments were performed in columns (to evaluate Cd leaching) and pots by growing rice plants (to study Cd accumulation in plants). Before their application, the tested amendments were thoroughly characterized using SEM, EDS and FT-IR spectroscopy. The leaching experiment indicates that the application of L or M/L significantly improved the pH of soil leachate collected at different time intervals. However, the use of M/L/S was found better in decreasing the Cd contents in collected leachate. The use of M/L efficiently decreased the DTPA metal extraction (0.19, 0.41, and 0.55 mg kg-1) as compared to the CK (0.35, 0.63, and 1.13 mg kg-1, respectively). The Cd speciation results depicted a 33% decrease in exchangeable Cd with M/L/S treatment when compared with control (55%). Moreover, the M/L/S treatment was more efficient in lowering the Cd phytoavailability and subsequent accumulation in rice grains (0.05, 0.09, and 0.08 mg kg-1). These findings demonstrate that the use of composite amendments is categorically effective as an in-situ remediation tool to decrease Cd leaching and availability in diverse contaminations.

The harmless treatment of heavy metal-enriched hyperaccumulator biomass is the main barrier to the industrialization of phytoremediation. Hydrothermal conversion of Sedum alfredii using different solvents (i.e ·H2O and HCl) at 210–300 ℃ was performed to investigate the behaviors of Cd and Zn, and the characteristics and potential application of the derived hydrochars were determined. Low temperature and HCl addition favored the removal of Cd/Zn from the solid phase. The highest removal efficiencies of Cd (95.0%) and Zn (89.3%) were achieved at 210 ℃ with the presence of HCl. The yield, pH, ash content, element concentration, functional groups, and crystalline minerals of the derived hydrochar were influenced by the reaction temperature and addition of HCl. The leaching risk of Cd and Zn was significantly reduced by hydrothermal conversion. The addition of HCl facilitated the immobilization of Zn, while it enhanced the mobility of Cd. Moreover, the hydrochar derived at 210 ℃ showed increased sorption capacity towards Cu, and the addition of HCl greatly improved the energy density of hydrochar. These results suggest that HCl-mediated hydrothermal conversion could be a promising technique to achieve the separation of Cd and Zn from hyperaccumulator biomass as well as the production of value-added hydrochar.

Plant growth regulators (PGRs) assisted phytoextraction was investigated as a viable phytoremediation technology to increase the phytoextraction efficiency in contaminated soils. This study aimed to evaluate the cadimum (Cd)/lead (Pb)/zinc (Zn) phytoextraction efficiency by a hyperaccumulator Sedum alfredii Hance (S. alfredii) treated with 9 PGRs, including indole-3-acetic acid (IAA), gibberellin (GA3), cytokinin (CKs), abscisic acid (ABA), ethylene (ETH), brassinosteroid (BR), salicylic acid (SA), strigolactones (SL) and jasmonic acid (JA), in slightly or heavily contaminated (SC and HC, respectively) soil. Results demonstrated that PGRs were able to improve S. alfredii biomass, the most significant increases were observed in GA3 and SL for HC soil, while for SC soil, IAA and BR exhibited positive effects. The levels of Cd, Pb and Zn in the shoots of S. alfredii treated with ABA and SL were noticeably greater than in the CK treatment in HC soil, while the uptake of metals were increased by IAA and CKs in SC soil. Combined with the results of biomass and metal contents in shoots, we found that ABA showed the highest Cd removal efficiency and the maximum Pb and Zn removal efficiency was observed with GA3, which was 62.99%, 269.23%, and 41.18%, respectively higher than the control in HC soil. Meanwhile, compared to control, the maximum removal efficiency of Cd by IAA treatment (52.80%), Pb by JA treatment (165.1%), and Zn by BR treatment (44.97%) in the SC soil. Overall, our results suggested that these PGRs, especially, ABA, SL, IAA, BR and GA3 had great potential in improving phytoremediation efficiency of S. alfredii grown in contaminated soils.

Heavy metal contamination of agricultural soils has posed a risk to environment and human health. The present study was conducted to assess the effectiveness of soil amendments for reducing cadmium (Cd) and lead (Pb) uptake by rice (Oryza sativa L) in a contaminated field. The soil amendments used include lime, DaSan Yuan (DASY), DiKang No.1 (DEK1), biochar, Fe-biochar, Yirang, phosphorus fertilizer, (Green Stabilizing Agent) GSA-1, GSA-2, GSA-3, and GSA-4, applied at 1% rate in a field experiment. The results exposed that GSA-4 treatment showed best effects on reducing Cd and Pb phytoavailability in soil and uptake by early rice. Linear increase in pH (i.e. 5.69 to 6.75) was recorded in GSA-4 amended soil from sowing to the 3rd month of growth season. GSA-4 decreased DTPA extractable contents of cadmium (Cd) from 0.324 to 0.136 mg kg−1 soil and lead (Pb) from 53.21 to 24.68 mg kg−1 soil at 90 days of amendment. Treatment with GSA-4 improved rice growth (56%) and grains yield (42%). The enhancement effects on grain yield may be result from the positive effects of GSA-4 application on increasing photosynthesis (116%) and transpiration rate (152%) as compared to the control. Significant reduction in Cd and Pb uptake in shoot (42% and 44%) and in grains (77 and 88%), was observed, respectively in GSA-4 treatment as compared with the control. Moreover, negative correlation was recorded between DTPA extractable Cd/Pb and soil pH that directly depended on applied amendments. In short, use of combined amendment (GSA-4) was more effective for immobilizing heavy metals in contaminated paddy field, and secures rice safe production, as compared other tested amendment products.

Willows (Salix spp.) have been regarded as one of interesting plants for phytofiltration of water contaminated with trace metals. In this paper, the clonal differences in tolerance and phytofiltration capacity of mixed metals (Mn, Zn, and Cu) were evaluated in greenhouse pilot-scale wetlands with a floating-support culture system. The results showed that broad clonal variations of biomass accumulation in response to mixed metals ranging from growth inhibition to stimulation. Clones differed in tolerance to multi-metals by tolerance index (TI) based on shoot and root biomass. We also found that wide variations in uptake and accumulation of three metals, which was related to species/clones and metal species. The willows showed high Mn and Zn translocation capacity from roots to leaves suitable for phytoextraction. In contrast, all clones had poor Cu translocation capacity, and Cu mainly retained in roots suitable for rhizofiltration. Among all Salix clones, clones SM30 and J903 had large phytofiltration potential for three metals with their high tolerance.

Two typical red soils were sequentially cultivated with celery (Apium graveolens L.) and Chinese cabbage (Brassica chinensis L.) in a greenhouse to determine the effect of lead (Pb) on plant availability of phosphorus (P) and potassium (K) in the soils. The concentrations of available P as estimated by the 0.05 mol L−1 HCl-0.025 mol L−1 (1/2 H2SO4) extraction and available K estimated by the NH4OAc extraction method in the crop-free soils were not affected by Pb treatment. Plant P concentrations in the above-ground part of celery and Chinese cabbage exposed to Pb were either lower or showed no significant difference to the control.

Cadmium accumulation in rice grains is highly dependent on its bioavailability that affected by various physicochemical properties and microbiological processes of soil. The rhizospheric bacterial communities of rice grown in contaminated soils by means of rice cultivars highly or weakly accumulating Cd in grains (HA and LA, respectively) were investigated. HA roots absorbed 7.26- and 2.25-fold more Cd than did LA roots at low (0.44 mg kg−1) and high (6.66 mg kg−1) soil Cd levels, respectively. Regardless of Cd levels, Cd bioavailability in the rhizosphere of HA was significantly higher than that of LA. Planting of rice and elevated Cd levels both significantly decreased bacterial α-diversity and altered bacterial community structure, with noticeable differences between the rice cultivars. Taxa specifically enriched in the HA rhizosphere (phyla Bacteroidetes, Firmicutes, and Deltaproteobacteria) can directly or indirectly participate in metal activation, whereas the LA rhizosphere was highly colonized by plant growth–promoting taxa (phyla Alphaproteobacteria and Gammaproteobacteria). The results indicate a potential association of Cd uptake and accumulation with rhizosphere bacteria in rice grown on a contaminated soil, thus providing baseline data and a new perspective on the maintenance of rice security.

Heavy metal pollution in peri-urban areas in China is serious and complex. We thus developed an integrated evaluation method to assess heavy metal pollution and potential health risk to residents in a typical peri-urban area with diverse anthropogenic emission sources and cropping systems. Ecological risk was evaluated using Nemerow's synthetical pollution index (Pn) and Potential ecological risk index (RI). Then polluted areas and responsible emission sources were identified by GIS mapping. Health risk caused by food intake and soil exposure was calculated by accounting for the influence of anthropogenic emissions and cropping systems. Agricultural soils in the study area were polluted by cadmium (Cd), mercury (Hg), lead (Pb), and arsenic (As). High concentrations mainly occurred near the mining area and along the roadsides. The accumulation of heavy metals in crops followed the order of tea leaves > rice grain > vegetables. The hazard index of potential human health risk caused by chronic soil exposure and food intake was 15.3, indicating obvious adverse health effects. 87.5% of health risk was attributed to food consumption, and significantly varied among different cropping systems with the decreasing order of rice (10.44) >vegetable (2.86) > tea (0.05). The integrated method of ecological and health risk index, which takes consideration of both anthropogenic emission and cropping system can provide a practical tool for evaluating of agricultural soil in the peri-urban area regrading different risk factors.

Phytoremediation is a green technology for heavy metal removal from contaminated soil, and its remediation efficiency and economic feasibility in field trial should be evaluated before large-scale application. However, there is still lacking relevant analysis, especially for phytoremediation with different cropping patterns. In the present study, we performed phytoremediation on slightly Cd-contaminated farmland soil via three cropping systems, i.e. Sedum alfredii monoculture, oilseed rape monoculture, and S. alfredii-oilseed rape intercropping. Dry weights of S. alfredii and oilseed rape were both enhanced under intercropping pattern, while the highest total Cd extraction amount (148 g ha−1) were observed under S. alfredii monoculture. Furtherly, a cost-benefit analysis via Monta Carlo simulation in a ten-year lifetime was conducted. The benefits of S. alfredii monoculture and intercropping schemes would offset the total costs in 6 and 8 years, respectively. S. alfredii monoculture achieved a higher net present value of 1.88 × 104 US$ as compared with intercropping (9.53 × 103 US$). These results indicate that S. alfredii monoculture scheme could be a promising phytoremediation strategy for slightly Cd-contaminated soil owing to better remediation efficiency and economic feasibility. Moreover, the enhancement in mechanization level and the reduction of seedling cost could further improve its economic viability.

Pyrolysis is a promising technique to achieve the sustainable utilization of heavy metal hyperaccumulator derived from phytoremediation of contaminated soils. To investigate the feasibility of synergistic treatment of hyperaccumulator and plastic waste (i.e. polyvinyl chloride, PVC), co-pyrolysis of Sedum alfredii and different mass percentages of PVC (5–25 wt%) was conducted at 300–900 °C in the present study. High pyrolysis temperature and low PVC addition amount (5 wt%) effectively promoted the volatilization of Cd and Zn from S. alfredii, while high PVC addition amount (15 wt% and 25 wt%) caused a significant suppression effect at insufficient pyrolysis temperatures. After PVC addition, the yields of biochar increased by 5.18–37.19% as compared with the theoretical values. However, the concentrations of Cd and Zn leached from biochar significantly elevated with increasing PVC addition amount, indicating that the addition of PVC improved the mobility of Cd and Zn in biochar. Moreover, S. alfredii derived biochars showed considerable sorption capacity for Cd (87.6–198.3 mg/g). These results imply that the addition of PVC has double-edged effects on heavy metal separation and biochar production during pyrolysis of Cd/Zn hyperaccumulator, and low PVC addition amount and sufficient pyrolysis temperature are beneficial for the further utilization of biochar.

Phytoremediation coupled with crop rotation (PCC) is a feasible strategy for remediation of contaminated soil without interrupting crop production. The objective of this study was to develop a PCC technology system for greenhouse fields co-contaminated with Cd and nitrate using hyperaccumulator Sedum alfredii. In this system, endophytic bacterium M002 inoculation, CO2 fertilization, and fermentation residue were continuously applied to improve the growth of S. alfredii, and low-accumulator Ipomoea aquatica and low-accumulator Brassica chinensis were rotated under reasonable water management. These comprehensive management practices were shown to increase S. alfredii biomass and Cd uptake and reduce Cd and nitrate concentration in I. aquatica and B. chinensis. This crop rotating system could remove 56.5% total Cd, 62.3% DTPA extractable Cd, and 65.4% nitrate, respectively, from the co-contaminated soil in 2 years of phytoremediation, and is an effective way of remediating moderately co-contaminated soil by Cd and nitrate.

Biochar has become eco-friendly amendment used for phytoavoidation with low cadmium (Cd) accumulating cultivars of crops to ensure food safety in Cd contaminated soils. In this study, biochar with different waste feedstock material were evaluated for their effectiveness on essential trace metals mobility, Cd bioavailability and its accumulation in two contrasting Cd accumulating cultivars of pak choi (Brassica rapa ssp. chinensis L.) grown in Cd contaminated Mollisol soil. A greenhouse experiment was conducted with plants grown in Cd contaminated soil that had been amended with biochar derived from barley straw, tomato green waste, chicken manure, duck manure and swine manure at application rate of 0%, 2.5% and 5.0% (w/w). The results showed that soil pH was significantly increased by all treatments. Biochar increased plant dry biomass, micronutrients bioavailability with significant differences in the Cd sorption capacity, with the effectiveness higher with increasing biochar application rate. However, tomato green waste (TGW) and chicken manure (CM) derived biochar were more effective than the other biochar in reducing Cd mobilization in soil by 35-54% and 26-43% and reduced its accumulation in shoots of pak choi cultivars by 34-76% and 33-72% in low Cd accumulator cultivar and 64-85% and 55-80% in high Cd accumulator cultivar than the control. Overall, results indicate that TGW and CM biochar can efficiently immobilize Cd, thereby reducing bioavailability in Cd contaminated Mollisol soil to ensure food safety.

Contamination of arable land with trace metals is a global environmental issue which has serious consequences on human health and food security. Present study evaluates the adsorption of cadmium (Cd) and lead (Pb) by using different quantities of composite of sepiolite, organic manure and lime (SOL) at field and laboratory scale (batch experiments). Characterization of SOL by SEM, EDS and FTIR spectroscopy revealed the presence of elemental and functional groups (hydroxyl, C⋯H and –COOH groups) on its surface. The field experiment was performed in a paddy field of gleysol having moderate contamination of Cd and Pb (0.64 mg kg−1 and 53.44 mg kg−1). Here, different rates of SOL (0.25, 0.5, 1, 1.5 and 2% w/w) were applied by growing low and high Cd accumulator rice cultivars. Application of SOL at 2% w/w showed considerable efficiency to increase soil pH (up to 19%) and to reduce available Cd (42–66%) and Pb (22–55%) as compared to the control. Moreover, its application reduced metal contents in roots, shoots and grains of rice by 31%, 36% and 72% (for Cd) and 41%, 81% and 84% (for Pb), respectively in low accumulator cultivar. Further, the batch sorption experiment was performed to evaluate the adsorption capacity of SOL in a wide range of contamination. Obtained sorption data was better fitted to the Langmuir equation. Our results highlight the strong efficiency of composite treatment for an enhanced in-situ metal immobilization under field and lab conditions. Further, applied treatments greatly reduced the metal contents in rice grains. In a nut shell, application of SOL in a contaminated gleysol should be considered for soil remediation and safe food production.

The present study evaluated the efficiency of pre-selected composite amendments (CA-1: biochar-lime-sepiolite-zeolite and CA-2: manure-lime-sepiolite) for immobilization and sorption of Cd and Pb in field and batch sorption experiments. The field experiment was performed in a co-contaminated clay purple soil (stagnic anthrosols). Along with a control experiment (T1), CA-1 and CA-2 were tested at different rates including 750, 1500, 3000 and 6000 kg ha−1 by growing wheat as the test crop. The obtained results revealed that the highest dose of both composites (T5: 6000 kg ha−1 and T9: 6000 kg ha−1) increased the soil pH to 6.85 and 6.81, respectively as compared to the control (5.63). DTPA-extractable Cd and Pb contents decreased with composite treatments (T7 and T4) at harvest stage samples. Metal fractionation depicted that application of amendments decreased the exchangeable fraction at harvesting stage. Application of CA-2 and CA-1 (3000 kg ha−1) significantly increased the plant biomass (by 28% and 24%, respectively) and grain yield (by 26% and 22%, respectively) of wheat. Furthermore, batch sorption experiment results revealed that Langmuir adsorption model better fitted the sorption results with R2 values ranging between 0.99 and 0.91 for Cd and Pb, respectively. CA-1 and CA-2 exhibited the maximum adsorption capacity for Cd with no significant difference among treatments but Pb adsorption capacity was highest in CA-1 followed by CA-2 and control. The results of our experiments revealed that the application of organics combined with inorganic materials enhanced Cd and Pb immobilization and sorption, consequently reducing metals availability in laboratory and field conditions. Moreover, for field trials, application of the composite amendments at 3000 kg ha−1 emerged as the suitable treatment for tested wheat-grown area.

Over the last decade, human population has been facing great challenges in ensuring appropriate supply of food free from cadmium (Cd) contamination. Selection of genetically low-Cd wheat (Triticum aestivum L.) genotypes, with a large biomass and high accumulation of Cd in straw but low-Cd concentration in grains, is an inventive approach of phytoremediation while keeping agricultural production in moderately contaminated soils. In this study, variations in Cd uptake and translocation among the 30 wheat genotypes in two different sites were investigated in field experiments. Significant differences in grain Cd concentration were observed between the two sites, with averaged values of 0.048 and 0.053 mg kg−1 DW, respectively. Based on straw Cd accumulation, grain Cd concentration, and TFrs, Bainong207 and Aikang58 for site A and Huaimai23 and Yannong21 for site B are promising candidates of low-Cd genotypes, which have considerable potential in achieving phytoremediation while keeping agricultural production on moderately or slightly Cd-polluted soil. The results indicate that it is possible to select the optimal low-Cd genotypes of wheat for different soil types by taking consideration of the effect of soil-wheat genotype interaction on grain Cd concentration.

Heavy metal removal by phytoremediation bears a great potential to decontaminate soils and Brassica juncea L. (Indian mustard) seems to be a possible candidate species for this purpose. A field experiment was conducted to compare the efficiency of eighty Indian mustard cultivars for phytoextraction of cadmium (Cd) and lead (Pb) from bimetal contaminated soil. Our results indicated that total Cd and Pb concentrations in the shoots and roots were in the range of 2.43 ± 0.00 to 0.31 ± 0.02 mg/kg and 2.94 ± 0.05 to 0.44 ± 0.03 mg/kg and 5.33 ± 0.76 to 0.47 ± 0.20 mg/kg and 3.78 ± 0.06 to 0.16 ± 0.08 mg/kg. Significant differences based on the translocation factors indicated that root-to-shoot transfer is higher for Pb (3.87 ± 0.12 to 0.48 ± 0.03) than Cd (3.38 ± 0.05 to 0.22 ± 0.01). Furthermore, significant correlations between dry weights, Cd and Pb concentrations and uptake in both shoots and roots were observed, but translocation factor showed a negative correlation with roots, but not in shoots. Among 80 genotypes of Indian mustard IM-25, IM-13 and IM-65 for Cd and IM-79, IM-24 and IM-32 for Pb seems to perform well for phytoextraction. The results of the field experiment suggest that certain Brassica juncea L. cultivars are suitable for removal of Cd and Pb in low to moderately contaminated soils.

Impact of different biochars supplemented (10% w/w) to promote vermicomposting of sewage sludge (SS) and kitchen waste (KW) mixture (SS + KW, 70:30) was studied on the growth, reproduction and survival of earthworms, and ultimately the quality of vermicompost. Four types of biochar used as secondary material for preincubation (16 days) and vermicomposting (30 days) were: pine tree biochar (PTB), poplar plant biochar (PPB), wetland plant biochar (WPB) and yard waste biochar (YWB). Preincubation and vermicomposting of biomass mixture were undertaken in 60 L and 2 L capacity round-shaped bioreactors, respectively. Samples of biomass undergoing degradation were drawn after every 2 days during preincubation and with 5 days interval during vermicomposting to analyze them for plant nutrients and heavy metals contents. Amendment of vermicompost substrate (SS + KW) with biochars; PTB, PPB, WPB and YWB increased the reproduction rate of earthworms (Eisenia fetida) by 44.6, 53.9, 29.3 and 38.8%, respectively as compared to control (no biochar, NB). There has been significant reduction in total content of Cd (0.2–5.1%), Cr (7.3–10.8%), Cu (3.1–7.4%), Mn (3.2–8.4%), Pb (9.0–45.9%) and Zn (1.1–5.7%) by the application of different biochars as compared to NB after vermicomposting. The SEM/EDS images also reflected reduced concentration of these heavy metals in the final vermicompost as compared to initial mixtures. Progressively, biochar amendments increased the concentration of all macronutrients, viz., TN (15.8–31.0%), TP (8.6–9.9%), TK (2.8–17.3%), Ca (4.1–9.9%) and Mg (0.8–12.2%); while, reduced the pH (1.9–2.3%), content of Na (6.6–22.3%), TOC (6.6–15.4%), OM (5.0–8.2%) and C:N ratio (2.6–18.9%). Earthworm body accumulation factor (BAF) of heavy metals was: Cd > Zn > Pb > Cu > Mn > Cr at the termination stage of experiment. In conclusion, amending the SS + KW mixture with 10% (w/w) PPB for vermicomposting rendered higher count of cocoons, growth rate and reproduction rate of earthworms, which ultimately produce nutrients-rich vermicompost lower in heavy metals.

Forty oilseed sunflower cultivars were screened in two soil types for phytoremediation of Cd coupled with maximum biomass yield and oil production. Several cultivars exhibited a significant difference in biomass and yield with enhanced uptake in shoots and low accumulation in roots from two Cd-contaminated soil types, an Oxisol and an Iceptisol. The Transfer Factor of Cd was >1 in several cultivars in both soil types, where as a significant difference in phytoextraction of Cd was observed in the Oxisol (acidic soil), greater than in the Inceptisol (alkaline soil). The results revealed that of the 40 cultivars, S9178, Huanong 667in the Oxisol and cvs. DW 667, HN 667, Huanong 667 and 668F1 in the Inceptisol showed a high biomass, better yield and enhanced accumulation of Cd in the shoots but a lesser accumulation in oil. The screened cultivar S 9178 produced the greatest amount of oil (55.6%) with 77% oleic acid, which makes it suitable for human consumption. Cultivar Huanong 667 was found to be the highest accumulating cultivar in both soil types. It is therefore suggested that some sunflower cultivars do exhibit phytoremediation potential together with agro-production potential.

Willows (Salix spp.) are characterized by having large biomass, high tolerance to flooding, and strong metal accumulation ability, exhibiting great promise in the phytoremediation of iron (Fe) from contaminated sites. In this study, the variation of Fe tolerance and accumulation in 24 willow clones was investigated with two levels of Fe(II)-EDTA, 0.025 mM (control) and 2.0 mM (treatment) by hydroponic system for 21 days in a greenhouse. Visual symptoms of Fe toxicity were observed in the leaves and roots of Fe sensitive clones. Clonal comparisons showed a great variation in Fe tolerance, and the high levels of Fe reduced biomass productions of most clones. Tolerance indexes (TIs) varied about five-fold based on shoot dry biomass and about six-fold based on root dry biomass among clones. Clones also exhibited a wide variation in Fe concentrations (mg g

Heavy metal pollution in soils has become an important concern for human health. Therefore, it is vital to develop suitable remediation strategies for contaminated soils. Oilseed rape tolerates high concentrations of heavy metals and is a promising candidate for the phytoextraction of cadmium (Cd) and lead (Pb) from metal-contaminated soils. A field experiment was conducted to evaluate 28 oilseed rape cultivars including Brassica napus L. and Brassica juncea L. for their ability to accumulate Cd and Pb. These cultivars were grown in a field co-contaminated with Cd (0.78 mg kg-1) and Pb (330 mg kg-1). The results showed that concentrations in shoots ranged from 1.22 to 3.01 mg kg-1 for Cd and from 10.8 to 29.5 mg kg-1 for Pb. Cadmium and Pb accumulations in shoots could reach 83.4 and 799 μg plant-1, respectively. The majority of translocation factors (TFs) for Cd (> 1.0) were higher than for Pb (≤ 1.0). However, concentrations of Cd and Pb in seeds were much lower, in the range of 0.04 to 0.21 mg kg-1 and 0.04 to 0.51 mg kg-1, respectively. The seed yields of oilseed rape varied from 1238 to 2904 kg ha-1, with a mean value of 2289 kg ha-1. Among the cultivars, three (OS-9, OS-12, and OS-15) were selected as Cd and Pb potential accumulators, with Cd accumulation in shoots being 2.74-3.70 times higher and Pb accumulation in shoots being 3.37-5.23 times higher as compared with the lowest accumulating cultivar. These selected cultivars (B. napus) have application potential for phytoextraction of Cd and Pb from polluted soils without stopping agricultural activities and accompanying food safety issues.

Phytoremediation coupled with agro-production is a sustainable strategy for remediation of toxic metal contaminated farmlands without interrupting crop production. In this study, high accumulating oilseed rape was rotated with low accumulating rice to evaluate the effects of crop rotation on growth performance and uptake of cadmium (Cd) in plants. In this system, oilseed rape was inoculated with plant growth promoting endophyte (PGPE) consortium, and rice was applied with soil composite amendment and foliar inhibitor. The results showed, compared with rice monoculture, crop rotation coupled with superposition measure has potential to enhance yield, biomass and nutritional quality of both crops, as well as to increase Cd uptake in non-edible tissues of oilseed rape and to reduce Cd concentration in individual parts of rice, thus accelerating phytoextraction and ensuring food safety. These comprehensive management practices removed 7.03 and 7.91% total Cd from two experiment fields, respectively, in three years phytoremediation. These results demonstrated a feasible technical mode for phytoremediation coupled with argo-production in slightly Cd contaminated field, and also provided useful information for further investigation of interaction mechanisms between the rotated crops and biofortification measures.

The present study investigated the effects of foliar application of zinc (Zn) and selenium (Se) on bioavailability of Zn and Se and toxicity of cadmium (Cd) and lead (Pb) to different water spinach ecotypes (LA and HA) grown in slightly (XZ) or moderately (LJY) contaminated fields via in vitro digestion combined with Caco-2/HL-7702 cell model. The obtained results revealed that foliar application of Zn and Se promoted yield, increased total, bioaccessible and bioavailable fractions of Zn and Se in plants, indicating that foliar application is a feasible way of biofortification. Although there was no significant effect on liver cell proliferation (MTT), membrane stability (LDH) and hepatocyte enzyme (ALT and AST) activities, the obvious ecotype and soil dependent fluctuations of lipid peroxidation (MDA) and antioxidant enzyme (SOD, POD and CAT) activities in serum highly suggest that the low accumulator and clean field should be used in agricultural production rather than the high accumulator and contaminated farmland. Moreover, foliar application of Zn and Se improved nutritional quality of all water spinach genotypes in both fields, including increased Fe, vitamin C, cellulose and chlorophyll, maintained concentrations of potassium (K), manganese (Mn), copper (Cu), protein, and nitrate. These results demonstrate that this agricultural management practice may prove to be an effective approach for minimizing health risk and alleviating "hidden hunger" in the developing countries.

Plant growth-promoting bacteria (PGPB) that inhabit hyperaccumulating plants assist cadmium (Cd) absorption, but the underlying mechanism has not been comprehensively studied. For this reason, we combined the fluorescence imaging, and transcriptomic and metabolomic methods in a Cd hyperaccumulator, Sedum alfredii, inoculated or not with PGPB Pseudomonas fluorescens. The results showed that the newly emerged lateral roots, that were heavily colonized by P. fluorescens, are the main entry for Cd influx in S. alfredii. Inoculation with P. fluorescens promoted a lateral root formation of its host plant, leading to a higher Cd phytoremediation efficiency. Furthermore, the plant transcriptome revealed that 146 plant hormone related genes were significantly up-regulated by the bacterial inoculation, with 119 of them showing a complex interaction, which suggests that a hormonal crosstalk participated root development. The targeted metabolomics analysis showed that P. fluorescens inoculation significantly increased indole acetic acid concentration and significantly decreased concentrations of abscisic acid, brassinolide, trans-zeatin, ethylene and jasmonic acid in S. alfredii roots, thereby inducing lateral root emergence. Altogether, our results highlight the importance of PGPB-induced lateral root formation for the increased Cd uptake in a hyperaccumulating plant.