1. A new method for biological synthesis of agriculturally relevant nanohydroxyapatite with elucidated effects on soil bacteria.
- Author
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Priyam A, Das RK, Schultz A, and Singh PP
- Subjects
- Bacteria growth & development, Calcium analysis, Gluconates analysis, Hydrodynamics, Microbial Sensitivity Tests, Nanoparticles ultrastructure, Particle Size, Phosphorus analysis, Spectroscopy, Fourier Transform Infrared, Static Electricity, X-Ray Diffraction, Agriculture methods, Bacteria drug effects, Durapatite pharmacology, Nanoparticles chemistry, Soil, Soil Microbiology
- Abstract
The study describes a novel and environment friendly route of biosynthesis of nanohydroxyapatite (nHAP). Bacillus licheniformis mediated synthesis of nHAP has been carried out with different phosphate concentrations (2%, 5%, 10% and 20% w/v) of potassium dihydrogen orthophosphate monobasic (K
2 HPO4 ). The synthesis is supported by a two-step mechanism - (i) solubilization of P by organic acids extracellularly secreted by the bacterial strain and (ii) gelation of P and Ca. The nHAP particles were characterized using electron microscopy and XRD analysis. Powdered crystalline particles with a size range of 30 ± 5 nm were obtained with shape and size dependent on phosphate concentrations. The particles showed no adverse effect on plant growth-promoting bacteria. Evaluation of nHAP prepared by this route with 2% P source provides scope for a wide range of applications, especially as a nano-fertilizer.- Published
- 2019
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