9 results on '"Opaliński, Łukasz"'
Search Results
2. N-glycosylation acts as a switch for FGFR1 trafficking between the plasma membrane and nuclear envelope
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Gregorczyk, Paulina, Porębska, Natalia, Żukowska, Dominika, Chorążewska, Aleksandra, Gędaj, Aleksandra, Malinowska, Agata, Otlewski, Jacek, Zakrzewska, Małgorzata, and Opaliński, Łukasz
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- 2023
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3. Differential regulation of fibroblast growth factor receptor 1 trafficking and function by extracellular galectins
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Kucińska, Marika, Porębska, Natalia, Lampart, Agata, Latko, Marta, Knapik, Agata, Zakrzewska, Małgorzata, Otlewski, Jacek, and Opaliński, Łukasz
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- 2019
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4. FGF1 Fusions with the Fc Fragment of IgG1 for the Assembly of GFPpolygons-Mediated Multivalent Complexes Recognizing FGFRs.
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Poźniak, Marta, Zarzycka, Weronika, Porębska, Natalia, Knapik, Agata, Marczakiewicz-Perera, Paulina, Zakrzewska, Malgorzata, Otlewski, Jacek, and Opaliński, Łukasz
- Subjects
CELL receptors ,CELL membranes ,CELL death ,BINDING sites ,CELL communication ,G proteins - Abstract
FGFRs are cell surface receptors that, when activated by specific FGFs ligands, transmit signals through the plasma membrane, regulating key cellular processes such as differentiation, division, motility, metabolism and death. We have recently shown that the modulation of the spatial distribution of FGFR1 at the cell surface constitutes an additional mechanism for fine-tuning cellular signaling. Depending on the multivalent, engineered ligand used, the clustering of FGFR1 into diverse supramolecular complexes enhances the efficiency and modifies the mechanism of receptor endocytosis, alters FGFR1 lifetime and modifies receptor signaling, ultimately determining cell fate. Here, we present a novel approach to generate multivalent FGFR1 ligands. We functionalized FGF1 for controlled oligomerization by developing N- and C-terminal fusions of FGF1 with the Fc fragment of human IgG1 (FGF1-Fc and Fc-FGF1). As oligomerization scaffolds, we employed GFPpolygons, engineered GFP variants capable of well-ordered multivalent display, fused to protein G to ensure binding of Fc fragment. The presented strategy allows efficient assembly of oligomeric FGFR1 ligands with up to twelve receptor binding sites. We show that multivalent FGFR1 ligands are biologically active and trigger receptor clustering on the cell surface. Importantly, the approach described in this study can be easily adapted to oligomerize alternative growth factors to control the activity of other cell surface receptors. [ABSTRACT FROM AUTHOR]
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- 2021
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5. Glycosylation of FGF/FGFR: An underrated sweet code regulating cellular signaling programs.
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Gędaj, Aleksandra, Gregorczyk, Paulina, Żukowska, Dominika, Chorążewska, Aleksandra, Ciura, Krzysztof, Kalka, Marta, Porębska, Natalia, and Opaliński, Łukasz
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HOMEOSTASIS , *POST-translational modification , *CELL communication , *FIBROBLAST growth factors , *GLYCOSYLATION , *CELL physiology , *GALECTINS - Abstract
Fibroblast growth factors (FGFs) and their receptors (FGFRs) constitute plasma-membrane localized signaling hubs that transmit signals from the extracellular environment to the cell interior, governing pivotal cellular processes like motility, metabolism, differentiation, division and death. FGF/FGFR signaling is critical for human body development and homeostasis; dysregulation of FGF/FGFR units is observed in numerous developmental diseases and in about 10% of human cancers. Glycosylation is a highly abundant posttranslational modification that is critical for physiological and pathological functions of the cell. Glycosylation is also very common within FGF/FGFR signaling hubs. Vast majority of FGFs (15 out of 22 members) are N-glycosylated and few FGFs are O-glycosylated. Glycosylation is even more abundant within FGFRs; all FGFRs are heavily N-glycosylated in numerous positions within their extracellular domains. A growing number of studies points on the multiple roles of glycosylation in fine-tuning FGF/FGFR signaling. Glycosylation modifies secretion of FGFs, determines their stability and affects interaction with FGFRs and co-receptors. Glycosylation of FGFRs determines their intracellular sorting, constitutes autoinhibitory mechanism within FGFRs and adjusts FGF and co-receptor recognition. Sugar chains attached to FGFs and FGFRs constitute also a form of code that is differentially decrypted by extracellular lectins, galectins, which transform FGF/FGFR signaling at multiple levels. This review focuses on the identified functions of glycosylation within FGFs and FGFRs and discusses their relevance for the cell physiology in health and disease. [Display omitted] • FGF/FGFR signaling plays a pivotal role in establishing and maintaining the cell and organism homeostasis. • Vast majority of FGF proteins and all FGFRs are N-glycosylated. • N- and O-glycosylation of FGFs affects their secretion, stability and function. • N-glycosylation of FGFRs facilitates intracellular trafficking of FGFRs to the cell surface and regulates FGFRs interaction with FGFs and co-receptors. • N-glycosylation of FGFs and FGFRs constitutes a layer of information differentially read by galectins and transformed into specific modulatory activities. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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6. Cross-Talk between Fibroblast Growth Factor Receptors and Other Cell Surface Proteins.
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Latko, Marta, Czyrek, Aleksandra, Porębska, Natalia, Kucińska, Marika, Otlewski, Jacek, Zakrzewska, Małgorzata, and Opaliński, Łukasz
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FIBROBLAST growth factor receptors ,CELL receptors ,MEMBRANE proteins ,CELL adhesion molecules ,POST-translational modification ,FIBROBLAST growth factors - Abstract
Fibroblast growth factors (FGFs) and their receptors (FGFRs) constitute signaling circuits that transmit signals across the plasma membrane, regulating pivotal cellular processes like differentiation, migration, proliferation, and apoptosis. The malfunction of FGFs/FGFRs signaling axis is observed in numerous developmental and metabolic disorders, and in various tumors. The large diversity of FGFs/FGFRs functions is attributed to a great complexity in the regulation of FGFs/FGFRs-dependent signaling cascades. The function of FGFRs is modulated at several levels, including gene expression, alternative splicing, posttranslational modifications, and protein trafficking. One of the emerging ways to adjust FGFRs activity is through formation of complexes with other integral proteins of the cell membrane. These proteins may act as coreceptors, modulating binding of FGFs to FGFRs and defining specificity of elicited cellular response. FGFRs may interact with other cell surface receptors, like G-protein-coupled receptors (GPCRs) or receptor tyrosine kinases (RTKs). The cross-talk between various receptors modulates the strength and specificity of intracellular signaling and cell fate. At the cell surface FGFRs can assemble into large complexes involving various cell adhesion molecules (CAMs). The interplay between FGFRs and CAMs affects cell–cell interaction and motility and is especially important for development of the central nervous system. This review summarizes current stage of knowledge about the regulation of FGFRs by the plasma membrane-embedded partner proteins and highlights the importance of FGFRs-containing membrane complexes in pathological conditions, including cancer. [ABSTRACT FROM AUTHOR]
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- 2019
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7. Targeting Cellular Trafficking of Fibroblast Growth Factor Receptors as a Strategy for Selective Cancer Treatment.
- Author
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Porębska, Natalia, Latko, Marta, Kucińska, Marika, Zakrzewska, Małgorzata, Otlewski, Jacek, and Opaliński, Łukasz
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FIBROBLAST growth factor receptors ,CHIMERIC proteins ,GROWTH factors ,CELL membranes ,FIBROBLAST growth factors - Abstract
Fibroblast growth factor receptors (FGFRs) in response to fibroblast growth factors (FGFs) transmit signals across the cell membrane, regulating important cellular processes, like differentiation, division, motility, and death. The aberrant activity of FGFRs is often observed in various diseases, especially in cancer. The uncontrolled FGFRs' function may result from their overproduction, activating mutations, or generation of FGFRs' fusion proteins. Besides their typical subcellular localization on the cell surface, FGFRs are often found inside the cells, in the nucleus and mitochondria. The intracellular pool of FGFRs utilizes different mechanisms to facilitate cancer cell survival and expansion. In this review, we summarize the current stage of knowledge about the role of FGFRs in oncogenic processes. We focused on the mechanisms of FGFRs' cellular trafficking—internalization, nuclear translocation, and mitochondrial targeting, as well as their role in carcinogenesis. The subcellular sorting of FGFRs constitutes an attractive target for anti-cancer therapies. The blocking of FGFRs' nuclear and mitochondrial translocation can lead to the inhibition of cancer invasion. Moreover, the endocytosis of FGFRs can serve as a tool for the efficient and highly selective delivery of drugs into cancer cells overproducing these receptors. Here, we provide up to date examples how the cellular sorting of FGFRs can be hijacked for selective cancer treatment. [ABSTRACT FROM AUTHOR]
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- 2019
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8. Engineered intrinsically fluorescent galectin-8 variants with altered valency, ligand recognition and biological activity.
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Kalka, Marta, Chorążewska, Aleksandra, Gędaj, Aleksandra, Żukowska, Dominika, Ciura, Krzysztof, Biaduń, Martyna, Gregorczyk, Paulina, Ptak, Julia, Porębska, Natalia, and Opaliński, Łukasz
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GREEN fluorescent protein , *CELL migration , *VALENCE (Chemistry) , *CELL communication , *GALECTINS - Abstract
Galectin-8 is a small soluble lectin with two carbohydrate recognition domains (CRDs). N- and C-terminal CRDs of Gal-8 differ in their specificity for glycan ligands. Here, we wanted to find out whether oligomerization of individual CRDs of galectin-8 affects its biological activity. Using green fluorescent protein polygons (GFPp) as an oligomerization scaffold, we generated intrinsically fluorescent CRDs with altered valency. We show that oligomers of C-CRD are characterized by significant cell surface affinity. Furthermore, the multivalency of the resulting variants has an impact on cellular activities such as cell signaling, heparin binding and proliferation. Our data indicates that tunable valence is a useful tool for modifying the biological activity of CRDs of galectins. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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9. Galectins as modulators of receptor tyrosine kinases signaling in health and disease.
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Porębska, Natalia, Poźniak, Marta, Matynia, Aleksandra, Żukowska, Dominika, Zakrzewska, Małgorzata, Otlewski, Jacek, and Opaliński, Łukasz
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GALECTINS , *CARRIER proteins , *KINASES , *TYROSINE , *POST-translational modification , *LECTINS , *GLYCOCALYX - Abstract
[Display omitted] • Receptor tyrosine kinases (RTKs) regulate pivotal cellular processes and are dysregulated in cancer. • RTKs are extensively glycosylated and this modification affects cellular functions of these receptors. • Galectins are carbohydrate binding proteins implicated in developmental processes and cancers. Galectins directly and indirectly affect RTKs, modulating RTKs activation, their cross-talk with other cell surface proteins and their cellular trafficking. • Galectins are responsible for oncogenic activation of RTKs. • RTK-galectin interplay constitutes an attractive target for development of anti-cancer therapies. Receptor tyrosine kinases (RTKs) constitute a large group of cell surface proteins that mediate communication of cells with extracellular environment. RTKs recognize external signals and transfer information to the cell interior, modulating key cellular activities, like metabolism, proliferation, motility, or death. To ensure balanced stream of signals the activity of RTKs is tightly regulated by numerous mechanisms, including receptor expression and degradation, ligand specificity and availability, engagement of co-receptors, cellular trafficking of the receptors or their post-translational modifications. One of the most widespread post-translational modifications of RTKs is glycosylation of their extracellular domains. The sugar chains attached to RTKs form a new layer of information, so called glyco-code that is read by galectins, carbohydrate binding proteins. Galectins are family of fifteen lectins implicated in immune response, inflammation, cell division, motility and death. The versatility of cellular activities attributed to galectins is a result of their high abundance and diversity of their cellular targets. A various sugar specificity of galectins and the differential ability of galectin family members to form oligomers affect the spatial distribution and the function of their cellular targets. Importantly, galectins and RTKs are tightly linked to the development, progression and metastasis of various cancers. A growing number of studies points on the close cooperation between RTKs and galectins in eliciting specific cellular responses. This review focuses on the identified complexes between galectins and RTK members and discusses their relevance for the cell physiology both in healthy tissues and in cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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