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This study examined the growth response of black tiger shrimp, Penaeus monodon, reared in indoor cement ponds fed at variable daily feeding frequencies. Five hundred healthy shrimp (1.26±0.37 g initial weight) were stocked in 12 cement ponds (5.5x2.5x1.5m). Three ponds in each group were assigned to four different feeding frequency i.e., two, three, four and six times/day designated as FF1, FF2, FF3 and FF4, respectively. Artificial diet (40% fishmeal protein) was used to feed at eight percent of total shrimp body weight per day for a total period of 56 days. The shrimp length and weight were measured fortnightly and the feeding rate was adjusted according to the new weight. At the end of the trial, all shrimp remained healthy and active with the survival rate of 70-85%. The specific growth rate (SGR) were the highest in groups FF3 (4.21±0.53) and FF4 (4.23±1.04), which were significantly higher than those in FF1 (3.82±0.49) and FF2 (4.02±1.03) groups. Whole body proximate composition (protein, ash, moisture and lipid) remained similar in all the treatment groups. It could be concluded that the black tiger shrimp, P. monodon, would be grown in indoor cement ponds effectively by feeding four times day-1 to achieve significantly better growth. [ABSTRACT FROM AUTHOR]

To explore the effect of concentrated dephenolic cottonseed protein (CDCP) partly instead of fish meal on the non-specific immune of Penaeus monodon, we designed a single factor test to study the four types of non-specific immunity of P. monodon hepatopancreas after acute salinity stress. A total number of 5,400 P. monodon were selected with an initial body weight of 1.76±0.15g and randomly divide into 6 groups (3 replicates per group, 900 tails per replicate). Shrimp were feed with two protein level diets (Diet A and Diet B). After 8 weeks of feeding, P. monodon were randomly selected with an average weight of 14.71±1.06g in each feed group, and subjected to acute salinity stress of 10ppt and 20ppt after temporary rearing, and the heat shock protein 70 (HSP70), Na+-K+-ATPase, cortisol and the content of alkaline phosphatase (ALP) were measured. The results of the study showed that when the CDCP partially replaced fish meal of P. monodon diet, the hepatopancreas HSP70, and the contents of Na+-K+-ATPase and cortisol were significantly higher than those of the control group. In the acute salinity change, there was no significant difference in the survival rate of P. monodon at 48h under the two salinity stresses, and the four immune indexes showed different trends. The expression level of Cortisol was more prominent than that of HSP70, which could better reflect the stress degree of P. monodon. Most of the indicators showed a trend of rising and then falling and then rising compared with the original level, and the changes of the four indicators of hepatopancreas in the Diet B group were significantly higher than those in the Diet A group at most time point of the two salinities. [ABSTRACT FROM AUTHOR]