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Minimization of excess sludge produced by wastewater treatment plants has become a topical theme nowadays. One of the most used approaches to achieve this aim is the anaerobic side-stream reactor (ASSR) process. This is considered affected by the hydraulic retention time (HRT) of the anaerobic reactor, the anaerobic sludge loading rate (ASLR) and the sludge interchange ratio (SIR), although, studies available in the literature did not reflect a clear relationship with the sludge minimization yields. To overcome this, a novel parameter namely anaerobic exposure time (AET) was defined and related to reduction of the observed yield coefficient (Y obs ) in a lab-scale plant implementing the ASSR process. Furthermore, the AET was validated by performing a detailed and thorough review of previous literature. Excess sludge production was successfully reduced (10-60 %) with the increase of the AET (7.9-13 h/d), although maintaining the same HRT in the ASSR and a constant sludge interchange ratio (SIR) (100 %). A strong correlation (Pearson = 0.763) was found between the AET, and the Y obs reduction reported in previous studies, also indicating a linear relationship (R 2 = 0.92) between these parameters. Contrarily, the correlation between the Y obs with the ASLR and the ASSR-HRT resulted moderate (Pearson = 0.186) or weak (Pearson=-0.346), respectively. Overall, while operating at low AET (< 6 h), maintenance and uncoupling metabolism were found the main sludge reduction mechanisms. Increasing the AET (>8 h) favoured the occurrence of extracellular polymeric substances (EPS) hydrolysis and endogenous decay mechanisms, which improved excess sludge reduction. To conclude, the AET could be considered a reliable parameter to be used for design or control purposes for the ASSR-based process., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

In the present research, insights about the mechanisms of excess sludge minimization occurring in an oxic-settling-anaerobic (OSA) were provided. The investigation involved two systems operating in parallel. In particular, a conventional activated sludge (CAS) system as control and a system implementing the OSA process both having a pre-denitrification scheme were considered. Five periods (P1-P5) were studied, during which several operating conditions and configurations were tested. Specifically, the hydraulic retention time (HRT) in the anaerobic reactor of the OSA system (P1 8 h, P2-P3 12 h, P4 8 h, P5 12 h) and the return sludge from the anaerobic to the anoxic (scheme A) (P1-P2) or aerobic (scheme B) mainstream reactors (P3-P5) were investigated. The results highlighted that the excess sludge production in the OSA was lower in all the configurations (12-41%). In more detail, the observed yield (Y obs ) was reduced from 0.50-0.89 gTSS gCOD -1 (control) to 0.22 -0.34 gTSS gCOD -1 in the OSA process. The highest excess sludge reduction (40%) was achieved when the OSA was operated according to scheme B and HRT of 12 h in the anaerobic reactor (P3). Generally, scheme A enabled the establishment of cell lysis and extracellular polymeric substances (EPS) destructuration, leading to a worsening of process performances when high anaerobic HRT (>8 h) was imposed. In contrast, scheme B enabled the establishment of maintenance metabolism in addition to the uncoupling metabolism, while cell lysis and EPS destruction were minimized. This allowed obtaining higher sludge reduction yield without compromising the effluent quality., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The integration of one anaerobic reactor in the mainstream (AMSR) of a pre-denitritication-MBR was evaluated with the aim to achieve simultaneous sludge minimization and phosphorous removal. The excess sludge production was reduced by 64% when the AMSR was operated under 8 h of hydraulic retention time (HRT). The highest nutrients removal performances referred to organic carbon (98%), nitrogen (90%) and phosphorous (97%) were obtained under 8 h of HRT. In contrast, prolonged anaerobic-endogenous conditions were found to be detrimental for all nutrients removal performances. Similarly, the lowest membrane fouling tendency (FR = 0.65∙10 11  m -1  d -1 ) was achieved under 8 h of HRT, whereas it significantly increased under higher HRT. The highest polyphosphate accumulating organisms kinetics were achieved under HRT of 8 h, showing very high exogenous P-release (46.67 mgPO 4 -P gVSS -1 h -1 ) and P-uptake rates (48.6 mgPO 4 -P gVSS -1 h -1 ), as well as a not negligible P-release rate under endogenous conditions at low COD/P ratio (≈1)., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

This study investigated the chance to couple the conventional Oxic Settling Anaerobic (OSA) process with a thermic treatment at moderate temperature (35 °C). The maximum excess sludge reduction rate (80%) was achieved when the plant was operated under 3 h of hydraulic retention time (HRT). Compared with the conventional OSA system, the thermic treatment enabled a further improvement in excess sludge minimization of 35%. The observed yield coefficient decreased from 0.25 gTSS gCOD -1 to 0.10 gTSS gCOD -1 when the temperature in the anaerobic reactor was increased to 35 °C, despite the lower HRT (3 h vs 6 h). Moreover, the thermic treatment enabled the decrease of filamentous bacteria, thereby improving the sludge settling properties. The thermic treatment enhanced the destruction of extracellular polymeric substances and the increase of endogenous decay rate (from 0.64 d -1 to 1.16 d -1 ) that reduced the biomass active fraction (from 22% to 4%)., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Biological nutrient removal performances and kinetics of autochthonous marine biomass in forms of activated sludge and aerobic granular sludge were investigated under different salinity and sludge retention time (SRT). Both the biomasses, cultivated from a fish-canning wastewater, were subjected to stepwise increases in salinity (+2 gNaCl L -1 ), from 30 gNaCl L -1 up to 50 gNaCl L -1 with the aim to evaluate the maximum potential in withstanding salinity by the autochthonous marine biomass. Microbial marine species belonging to the genus of Cryomorphaceae and of Rhodobacteraceae were found dominant in both the systems at the maximum salinity tested (50 gNaCl L -1 ). The organic carbon was removed with a yield of approximately 98%, irrespective of the salinity. Similarly, nitrogen removal occurred via nitritation-denitritation and was not affected by salinity. The ammonium utilization rate and the nitrite utilization rate were approximately of 3.60 mgNH 4 -N gVSS -1 h -1 and 10.0 mgNO 2 -N gVSS -1 h -1 , respectively, indicating a high activity of nitrifying and denitrifying bacteria. The granulation process did not provide significant improvements in the nutrients removal process likely due to the stepwise salinity increase strategy. Biomass activity and performances resulted affected by long SRT (27 days) due to salt accumulation within the activated sludge flocs and granules. In contrast, a lower SRT (14 days) favoured the discharge of the granules and flocs with higher inert content, thereby enhancing the biomass renewing. The obtained results demonstrated that the use of autochthonous-halophilic bacteria represents a valuable solution for the treatment of high-strength carbon and nitrogen saline wastewater in a wide range of salinity. Besides, the stepwise increase in salinity and the operation at low SRT enabled high metabolic activity and to avoid excessive accumulation of salt within the biomass aggregates, limiting their physical destructuration due to the increase in loosely-bound exopolymers., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

The modification of the physical properties of aerobic granular sludge treating fish-canning wastewater is discussed in this paper. The structure and composition of the Extracellular Polymeric Substances (EPSs) were analyzed at different salinity levels and related to granules stability. Results outlined that the total EPSs content increased with salinity, despite the EPSs increment was not proportional to the salt concentration. Moreover, the EPSs structure was significantly modified by salinity, leading to a gradual increase of the not-bound EPSs fraction, which was close to the 50% of the total EPSs content at 75gNaClL -1 . The increasing salt concentration modified also the EPSs composition, causing the gradual reduction of protein content resulting in a decrease of granule hydrophobicity. The results pointed out that the granules stability significantly reduced above 50gNaClL -1 , suggesting the existence of a salinity threshold above which granules stability is compromised., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Results obtained from three aerobic granular sludge reactors treating brewery wastewater are presented. Reactors were operated for 60d days in each of the two periods under different cycle duration: (Period I) short 6h cycle, and (Period II) long 12h cycle. Organic loading rates (OLR) varying from 0.7kgCODm -3 d -1 to 4.1kgCODm -3 d -1 were tested. During Period I, granules successfully developed in all reactors, however, results revealed that the feast and famine periods were not balanced and the granular structure deteriorated and became irregular. During Period II at decreased 12h cycle time, granules were observed to develop again with superior structural stability compared to the short 6h cycle time, suggesting that a longer starvation phase enhanced production of proteinaceous EPS. Overall, the extended famine conditions encouraged granule stability, likely because long starvation period favours bacteria capable of storage of energy compounds., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

This work aims to investigate the stability of aerobic granular sludge in the long term, focusing on the clogging of the granular sludge porosity exerted by the extracellular polymeric substances (EPSs). The effects of different cycle lengths (short and long-term cycle) on the granular sludge stability were investigated. Results obtained outlined that during the short duration cycle, the formation and breakage of the aerobic granules were continuously observed. During this period, the excess of EPS production contributed to the clogging of the granules porosity, causing their breakage in the long run. During the long-duration cycle, the extended famine period entailed a greater EPSs consumption by bacteria, thus limiting the clogging of the porosity, and allowed obtaining stable aerobic granules. Reported results demonstrated that an excess in EPSs content could be detrimental to the stability of aerobic granular sludge in the long-term., (Copyright © 2016 Elsevier Ltd. All rights reserved.)

The recovery of biopolymers from sewage sludge could be a crucial step in implementing circular economy principles in wastewater treatment plants (WWTP). In this frame, the present study was aimed at evaluating the simultaneous production of polyhydroxyalkanoates (PHA) and extracellular polymeric substances (EPS) obtainable from the treatment of agro-industrial wastewater. Two biological enrichment systems, aerobic granular sludge (AGS) and a conventional activated sludge operating as a sequencing batch reactor (SBR), were monitored for 204 and 186 days, respectively. The maximum biopolymers accumulation capacity was close to 0.60 mgPHA-EPS gVSS−1 in the AGS when operating at 3 kgCODm−3d−1, whereas in the SBR, it was about half (0.35 mgPHA-EPS gVSS−1). Biopolymers extracted from the AGS were mainly constituted by EPS (>70%), whose percentage increased up to 95% with the OLR applied in the enrichment reactor. In contrast, SBR enabled obtaining a higher PHA production (50% of the biopolymers). Results suggested that organic carbon was mainly channeled toward metabolic pathways for extracellular storing in AGS, likely due to metabolic stressors (e.g., hydraulic selection pressure, shear forces) applied for promoting aerobic granulation. [ABSTRACT FROM AUTHOR]

Updating of the current Urban Waste Water Treatment Directive (91/271/EEC) will demand stricter regulations for nutrients removal. In this frame, wastewater treatment plants (WWTPs) of small-to-medium potential will face new challenges for achieving process intensification. Integrating intermittent aeration (IA) and integrated fixed-film activated sludge (IFAS) technologies could be a promising solution to meet such requirements. This study analyzed how IA cycles affected nutrients removal in IFAS reactors with different biofilm carriers (e.g., plastic and sponge media). The plants responses to different carbon/nitrogen/phosphorous (C/N/P) ratios were evaluated while operating under low sludge retention time (SRT) to simulate overloaded conditions. A short IA cycle (1 h) with an aeration/not aeration ratio of 2:1 enabled high organic carbon and nitrification performances when operating at high C/N/P (11.8/1/1), whereas low denitrification and phosphorous removal yields were obtained because of the short not-aerated phase. Decreasing C/N ratio (8.8/1/1) without changing the IA cycle resulted in nitrification worsening because of the reduced metabolic kinetics of biofilm. Under such load conditions, a higher IA cycle (2 h) was necessary to improve process performance. A longer not-aerated phase was also positive for denitrification and phosphorous removal because of the establishment of anoxic and anaerobic environments within the bulk and inner biofilm layers. Besides, results suggested that sponge carriers offered advantages over plastic ones, enabling a higher biofilm retention capacity, better nutrient removal, as well as robustness and resilience to operating condition changes. This would result in simpler management systems for implementing the IA process, thus reducing process complexity and costs. • Integration of IA and IFAS technologies was studied for overloaded WWTPs. • Short 1-h IA cycles (2:1 aeration ratio) favoured organic removal and nitrification. • Longer IA cycles (2 h) improved overall process performances under lower C/N. • Sponge carriers improved biofilm retention, nutrient removal, and process stability. • Sensor-controlled aeration is recommended to cope with daily flow variability. [ABSTRACT FROM AUTHOR]

The present study investigated the combined production of reclaimed water for reuse purposes and polyhydroxyalkanoates (PHA) from an agro-food industrial wastewater. A pilot plant implementing a two-stage process for PHA production was studied. It consisted of a mainstream sequencing batch membrane bioreactor (SBMBR) in which selection of PHA-accumulating organisms and wastewater treatment were carried out in, and a side-stream fed-batch reactor (FBR) where the excess sludge from the SBMBR was used for PHA accumulation. The performance of the SBMBR was compared with that of a conventional sequencing batch reactor (SBR) treating the same wastewater under different food to microorganisms' ratios (F/M) ranging between 0.125 and 0.650 kgCOD kgTSS−3 d−1. The SBMBR enabled to obtain very high-quality effluent in compliance with the relevant national (Italy) and European regulations (Italian DM 185/03 and EU, 2020/741) in the field of wastewater reclamation, whereas the performances in the SBR collapsed at F/M higher than 0.50 kgCOD kgTSS−1d−1. A maximum intracellular storage of 45% (w/w) and a production yield of 0.63 gPHA L−1h−1 were achieved when the SBMBR system was operated with a F/M ratio close to 0.50 kgCOD kgTSS−1d−1. This resulted approximately 35% higher than those observed in the SBR, since the ultrafiltration membrane avoided the washout of dispersed and filamentous bacteria capable of storing PHA. Furthermore, while maximizing PHA productivity in conventional SBR systems led to process dysfunctions, in the SBMBR system it helped mitigate these issues by reducing membrane fouling behaviour. The results of this study supported the possibility to achieve combined recovery of reclaimed water and high-value added bioproducts using membrane technology, leading the way for agro-food industrial wastewater valorization in the frame of a circular economy model. [Display omitted] • A mainstream SBMBR was used for the combined recovery of treated water and PHA. • SBMBR effluent complied with reuse limits set by the current European regulations. • PHA performance (45% w/w and 0.63 gPHA L−1h−1) were higher in the SBMBR than the SBR. • Competitive reactions to PHA accumulation (EPS synthesis) were limited in the SBMBR. • PHA accumulation significantly reduced the membrane fouling tendency. [ABSTRACT FROM AUTHOR]

The paper focused on the evolution and the comparison of the extracellular polymeric substances (EPSs) content during the granulation process in two Granular Sequencing Batch Airlift Reactors (GSBAR) (3,5 L) fed with synthetic (R1) and industrial wastewater (R2). The results showed that in both the reactors the EPSs, in particular proteins (PN), were mainly produced during the feast phase because of the high substrate availability, especially under conditions of metabolic stress. Then, the EPSs content reduced during the famine period, because of biodegradation by bacteria. More in detail, during the granulation process, a greater polysaccharides (PS) consumption occurred in both reactors, whereas the PN content reduced significantly only in R2. As a result, the PN/PS ratio increased significantly during the granules formation, confirming the key role of proteins on the granulation process. In R2, the granules produced a greater amount of PN on average, and this resulted in a more rapid granulation. Nevertheless, due to the several consumption of PN during the famine period, the granules in R2 resulted weaker and less dense compared with the granules in R1. For this reason, the granules in R2 were more susceptible to breakage. Overall, although the industrial wastewater favored a more rapid granulation, the excessive consumption of the EPSs, led to the structural weakening of the granules. [ABSTRACT FROM AUTHOR]

This study analyzed the performances of a hybrid membrane aerated biofilm reactor (MABR) pilot plant in terms of nutrients removal of the attached growth and suspended biomass in comparison with a conventional activated sludge (CAS) system at different sludge retention time (SRT) (20−3) days. Overall, the MABR showed better performances than the CAS in terms of TSS (86% vs 79%), COD (89% vs 85%) and total nitrogen (80% vs 65%). The minimum SRT for achieving complete nitrification in the MABR was close to 3 days, corresponding to a SRT in the aerobic compartment of 1.9 days, whereas in the CAS it was equal to 8 days (aerobic SRT of 4.8 days). Nitrification rate in biofilm was on average equal to 0.40 gNH 4 -N h−1 (2.40 gNH 4 -N m−2d−1). Its contribution to the overall nitrification in the MABR plant was 25–30% on average, although it increased when the SRT was decreased. Particle size distribution and microscopic analyses showed particles of biofilm detached from the membrane of the MABR. The seeding effect allowed sustaining nitrification of the suspend biomass at very low SRT. The nitrification rate observed in the suspended biomass in the MABR slightly decreased from 3.42 mgNH 4 -N gVSS−1 h−1 to 2.87 mgNH 4 -N gVSS−1 h−1 when the SRT was decreased from 20 days and 3 days, whereas in the CAS it collapsed from 2.33 mgNH 4 -N gVSS−1 h−1 to 0.47 mgNH 4 -N gVSS−1 h−1, because of nitrifying washout. Moreover, the biofilm detachment involved a positive effect in settling properties of the suspended biomass. [Display omitted] • A hybrid MABR pilot plant fed with real wastewater was monitored for 304 days. • High nitrification efficiency was obtained in MABR with aerobic SRT of 1.8 days. • Seeding effect enabled high nitrification in MABR below the washout SRT of CAS. • Biofilm performed stable nitrification rate of 2.40 gNH 4 -N m−2d−1 regardless of SRT. • Biofilm detachment improved settling properties of the suspended biomass. [ABSTRACT FROM AUTHOR]

The present study has focused on the mainstream integration of polyhydroxyalkanoate (PHA) production with industrial wastewater treatment by exploiting three different technologies all operating in sequencing batch reactors (SBR): conventional activated sludge (AS-SBR), membrane bioreactor (AS-MBR) and aerobic granular sludge (AGS). A full aerobic feast/famine strategy was adopted to obtain enrichment of biomass with PHA-storing bacteria. All the systems were operated at different organic loading (OLR) rate equal to 1-2-3 kgCOD/m3∙d in three respective experimental periods. The AS-MBR showed the better and stable carbon removal performance, whereas the effluent quality of the AS-SBR and AGS deteriorated at high OLR. Biomass enrichment with PHA-storing bacteria was successfully obtained in all the systems. The AS-MBR improved the PHA productivity with increasing OLR (max 35% w/w), whereas the AS-SBR reduced the PHA content (max 20% w/w) above an OLR threshold of 2 kgCOD/m3∙d. In contrast, in the AGS the increase of OLR resulted in a significant decrease in PHA productivity (max 14% w/w) and a concomitant increase of extracellular polymers (EPS) production (max 75% w/w). Results demonstrated that organic carbon was mainly driven towards the intracellular storage pathway in the AS-SBR (max yield 51%) and MBR (max yield 61%), whereas additional stressors in AGS (e.g., hydraulic selection pressure, shear forces) induced bacteria to channel the COD into extracellular storage compounds (max yield 50%) necessary to maintain the granule's structure. The results of the present study indicated that full-aerobic feast/famine strategy was more suitable for flocculent sludge-based technologies, although biofilm-like systems could open new scenarios for other biopolymers recovery (e.g., EPS). Moreover, the AS-MBR resulted the most suitable technology for the integration of PHA production in a mainstream industrial wastewater treatment plant, considering the greater process stability and the potential reclamation of the treated wastewater. [Display omitted] • A mainstream process for PHA production was integrated with wastewater treatment. • A comparison between AS, AGS and MBR as the enrichment/treatment stage was assessed. • MBR enabled the highest PHA production potential and effluent water eligible for reuse. • Biomass enrichment under full aerobic in AGS promoted extracellular storage pathways. • Integration of PHA production in the mainstream of a WWTP resulted feasible using MBR. [ABSTRACT FROM AUTHOR]