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Occurrence of root rot disease in sesame has become a major constraint for cultivation of sesame in Junagadh district of Gujarat State (India). Considering the fact, the following investigation was carried out for this pathological problem. The efficacy of various botanicals were evaluated against Macrophomina phaseolina (Tassi.) Goid causing root rot of sesame. The phytoextracts of nine plant species were evaluated in vitro by poisoned food technique against M. phaseolina. The extract of garlic cloves (Allium sativum L.) was proved excellent with maximum inhibiting (77.65%) mycelial growth and scanty sclerotial formation followed by onion bulb extract (Allium cepa L.) (63.98%). while least growth inhibition (32.34%) was recorded in ginger rhizome extract. [ABSTRACT FROM AUTHOR]