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In this study, an embryogenic callus induction and proliferation system for Hippeastrum vittatum was established, with the tender bulbs as expiants. And then the sensitivity of the expiants and calli to kanamycin and hygromycin was evaluated. The results suggested that the embryogenic calli were induced from tender bulbs and cultured in Murashige and Skoog (MS) medium supplemented with 0.5 mg/L N-phenyl-N'-l, 2,3-thiadiazol-5-ylurea (TDZ), 1.0 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), and 30 g/L sucrose (pH5.8) in the dark at 25 ± 1°C. Further study of the influence of kanamycin and hygromycin on callus induction and multiplication showed that, the lethal doses of kanamycin and hygromycin to bulb expiants were 100 and 30 mg/L, respectively. All expiants of H. vittatum died on the medium supplemented with 100 mg/L kanamycin or 30 mg/L hygromycin at the induction stage, and callus proliferation was completely inhibited by 100 mg/L kanamycin or 25 mg/L hygromycin, and all the calli died at last. These results will provide important reference for further studies of transgenic H. vittatum. [ABSTRACT FROM AUTHOR]

In this study, embryogenic cell aggregates obtained from the established embryogenic cell suspension culture system of Anthurium andraeanum 'Alabama'' were used as experimental materials to investigate the effects of kanamycin and hygromycin on survival rate of cell aggregates, somatic embryogenesis and plantlet regeneration of A. andraeanum. According to the results, at the embryonic cell propagation stage, lethal doses of kanamycin and hygromycin to embryogenic cell aggregates of A. andraeanum were 200 and 60 mg/L, respectively; at the differentiation stage, either 150 mg/L kanamycin or 40 mg/L hygromycin could inhibit somatic embryogenesis; either 100 mg/L kanamycin or 20 mg/L hygromycin could inhibit plantlet regeneration. These results provided important reference for further studies of transgenic A. andraeanum. [ABSTRACT FROM AUTHOR]