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The quest to improve the performance of organic light-emitting diodes (OLEDs) has led to the exploration of new materials with properties like interfacial dipole, excitons generation, and bandgap alignment. Here, we exploit these strategies by investigating the interaction of the antibiotic ampicillin with a widely used optoelectronic material, to fabricate state-of-the-art OLEDs. The charge distribution on the ampicillin molecule facilitates the generation of an interfacial dipole with a large magnitude. The optimum fusion of the two materials provides an enhanced bandgap alignment, charge balance and J/H-aggregated excitons. Values of current efficiency (120 cdA−1), external quantum efficiency (~35%) and power efficiency (70 lmW−1) are demonstrated. The cross-evaluation of performance with penicillin devices indicates the significance of ampicillin's specific molecular structure in improving performance. The detailed investigations demonstrate that ampicillin has superior optoelectronic properties with high potential to contribute extensively in OLEDs and photovoltaics. The need to move away from silicon-based semiconductors for the electronic industry has yielded significant developments in the use of organic materials for devices. The authors present a method to enhance organic light-emitting diodes (OLEDs) performance by adding a common antibiotic, ampicillin into a common organic semiconductor and achieving a maximum external quantum efficiency of 35%. [ABSTRACT FROM AUTHOR]