1. Comprehensive semen examination in patients with pancreatic-sufficient and pancreatic-insufficient cystic fibrosis.
- Author
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Sedova AO, Shtaut MI, Bragina EE, Sorokina TM, Shmarina GV, Andreeva MV, Kurilo LF, Krasovskiy SA, Polyakov AV, and Chernykh VB
- Subjects
- Humans, Male, Adult, Spermatozoa pathology, Oligospermia pathology, Oligospermia genetics, Azoospermia pathology, Azoospermia genetics, DNA Fragmentation, Infertility, Male pathology, Infertility, Male genetics, Infertility, Male diagnosis, Infertility, Male etiology, Young Adult, Cystic Fibrosis pathology, Cystic Fibrosis genetics, Cystic Fibrosis complications, Semen Analysis
- Abstract
We examined a cohort of 93 cystic fibrosis (CF) male patients who were pancreatic-sufficient (PS-CF; n=40) or pancreatic-insufficient (PI-CF; n = 53). Complex semen examination was performed, including standard semen analysis, quantitative karyological analysis (QKA) of immature germ cells (IGCs), transmission electronic microscopy (TEM), biochemical analysis, and sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL) assay. Azoospermia was diagnosed in 83 (89.2%) patients. The other 10 (10.8%) patients were found to be nonazoospermic and showed various spermatological diagnoses (asthenozoospermia, n = 2; asthenoteratozoospermia, n = 3; oligoasthenozoospermia, n = 1; oligoasthenoteratozoospermia, n = 3; and normozoospermia, n = 1) with no specific morphological abnormalities. Oligospermia was detected in 89.2% azoospermic and 30.0% nonazoospermic patients. Low seminal pH (<7.0) was found in 74 (89.2%) of 83 azoospermic patients. Moderate leukocytospermia (2.0 × 10 6 -2.2 × 10 6 ml -1 ) was revealed in 2.4% azoospermic and 40.0% nonazoospermic semen samples. The signs of partial meiotic arrest at prophase I were found in 4 of 6 nonazoospermic patients examined by QKA of IGCs. The content of fructose and citrate was low in oligospermic and normal in nonoligospermic semen samples. An increased percentage (>30%) of spermatozoa with noncondensed ("immature") chromatin was revealed in 2 of 6 nonazoospermic semen samples analyzed by TEM., (Copyright © 2023 Copyright: © The Author(s)(2023).)
- Published
- 2023
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