Search

In our earlier study, a bacterial isolate,Bacillus cereus(strain CM100B) was reported to detoxify selenite oxyions with concomitant generation of amorphous selenium (Se0) nanospheres. A whole genome transcriptional analysis was performed to study the genome wide stress response of this bacterium to elevated concentrations of selenite and arsenite oxyions. Data analysis revealed that various genes related to metabolic pathways, transcription, DNA repair and replication and oxidative stress were activated symptomatic of the various stress related response. It seems that in order to cope with the oxidative stress that results from the exposure to oxidants such as selenite and arsenite, bacteria have evolved multiple, and often redundant, defense systems to eliminate the reactive oxyion species (ROS) from the cell. [ABSTRACT FROM AUTHOR]

Background: Microorganisms that are exposed to pollutants in the environment, such as metals/metalloids, have a remarkable ability to fight the metal stress by various mechanisms. These metal-microbe interactions have already found an important role in biotechnological applications. It is only recently that microorganisms have been explored as potential biofactories for synthesis of metal/metalloid nanoparticles. Biosynthesis of selenium (Se0) nanospheres in aerobic conditions by a bacterial strain isolated from the coalmine soil is reported in the present study. Results: The strain CM100B, identified as Bacillus cereus by morphological, biochemical and 16S rRNA gene sequencing [GenBank:GU551935.1] was studied for its ability to generate selenium nanoparticles (SNs) by transformation of toxic selenite (SeO32-) anions into red elemental selenium (Se0) under aerobic conditions. Also, the ability of the strain to tolerate high levels of toxic selenite ions was studied by challenging the microbe with different concentrations of sodium selenite (0.5 mM-10 mM). ESEM, AFM and SEM studies revealed the spherical Se0 nanospheres adhering to bacterial biomass as well as present as free particles. The TEM microscopy showed the accumulation of spherical nanostructures as intracellular and extracellular deposits. The deposits were identified as element selenium by EDX analysis. This is also indicated by the red coloration of the culture broth that starts within 2-3 h of exposure to selenite oxyions. Selenium nanoparticles (SNs) were further characterized by UV-Visible spectroscopy, TEM and zeta potential measurement. The size of nanospheres was in the range of 150-200 nm with high negative charge of -46.86 mV. Conclusions: This bacterial isolate has the potential to be used as a bionanofactory for the synthesis of stable, nearly monodisperse Se0 nanoparticles as well as for detoxification of the toxic selenite anions in the environment. A hypothetical mechanism for the biogenesis of selenium nanoparticles (SNs) involving membrane associated reductase enzyme(s) that reduces selenite (SeO32-) to Se0 through electron shuttle enzymatic metal reduction process has been proposed. [ABSTRACT FROM AUTHOR]