Search

A novel exopolysaccharide-producing bacterium, designated strain 9a2(T), was isolated from Pacific Ocean sediment. The strain was Gram-stain-negative, motile, strictly aerobic, oxidase- and catalase-positive, and required NaCl for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile consisted mainly of C16 : 1ω7c, C18 : 1ω9c and C16 : 0. The DNA G+C content was 46.6 mol%. 16S rRNA gene sequence analysis suggested that strain 9a2(T) is a member of the genus Alteromonas . Strain 9a2(T) exhibited closest phylogenetic affinity to Alteromonas macleodii NBRC 102226(T) (99.3% 16S rRNA gene sequence similarity), A. marina SW-47(T) (99.3%), A. litorea TF-22(T) (99.0%), A. australica H17(T) (98.7%), A. simiduii BCRC 17572(T) (98.5%), A. stellipolaris LMG 21861(T) (98.3%) and A. hispanica F-32(T) (98.2%). The DNA-DNA reassociation values between strain 9a2(T) and A. macleodii JCM 20772(T), A. marina JCM 11804(T), A. litorea JCM 12188(T), A. australica CIP 109921(T), A. simiduii JCM 13896(T), A. stellipolaris LMG 21861(T) and A. hispanica LMG 22958(T) were below 70%. Strain 9a2(T) contained phosphatidylethanolamine, phosphatidylglycerol and an unidentified polar lipid. Owing to differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA relatedness data, the isolate merits classification as representing a novel species, for which the name Alteromonas gracilis sp. nov. is proposed. The type strain of this species is 9a2(T) ( =JCM 30236(T) =NCIMB 14947(T))., (© 2015 IUMS.)

A novel marine bacterium, designated strain 4k5(T), was isolated from a sediment sample of the Pacific Ocean. The strain was Gram-stain-negative, strictly aerobic, non-motile, oxidase-positive and catalase-positive and required Na(+) for growth. Its major isoprenoid quinone was ubiquinone 8 (Q-8), and its cellular fatty acid profile consisted mainly of C18 : 1v9c (71.4%), C16 : 1v7c (9.1%) and C18 : 0. The DNA G+C content was 45.3 mol%. 16S rRNA gene sequence analysis suggested that strain 4k5(T) is a member of the genus Psychrobacter . Strain 4k5(T) exhibited the closely phylogenetic affinity to Psychrobacter pacificensis IFO 16270(T) (99.4% 16S rRNA gene sequence similarity), P. piscatorii T-3-2(T) (97.7%), P. nivimaris 88/2-7(T) (97.7%), P. celer SW-238(T) (97.7%), P. aestuarii SC35(T) (97.6%) and P. vallis CMS39(T) (97.6%). DNA-DNA hybridization between strain 4k5(T) and P. pacificensis NBRC 103191(T), P. piscatorii JCM 15603(T). P. nivimaris DSM 16093(T), P. celer JCM 12601(T), P. aestuarii JCM 16343(T) and P. vallis DSM 15337(T) was 42.5, 47.0, 38.1, 23.7, 9.0 and 27.4%, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on the 16S rRNA gene sequence and DNA-DNA relatedness data, the isolate merits classification within a novel species, for which the name Psychrobacter oceani sp. nov. is proposed. The type strain is 4k5(T) ( = JCM 30235(T) =NCIMB 14948(T))., (© 2015 IUMS.)

A novel exopolysaccharide-producing bacterium, designated strain SE3(T), was isolated from Pacific Ocean sediment. The strain was Gram-stain-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na(+) for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The DNA G+C content was 46.9 mol%. 16S rRNA gene sequence analysis suggested that strain SE3(T) is a member of the genus Pseudoalteromonas. Strain SE3(T) exhibited close phylogenetic affinity to Pseudoalteromonas arabiensis JCM 17292(T) (99.0 % 16S rRNA gene sequence similarity), Pseudoalteromonas lipolytica LMEB 39(T) (98.39 %) and Pseudoalteromonas donghaensis HJ51(T) (97.65 %). The DNA-DNA reassociation values between strain SE3(T) and P. arabiensis JCM 17292(T), P. lipolytica JCM 15903(T) and P. donghaensis LMG 24469(T) were 31, 26 and 44 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA relatedness data, the new isolate merits classification as a representative of novel species, for which the name Pseudoalteromonas shioyasakiensis is proposed. The type strain is SE3(T) ( = JCM 18891(T) = NCIMB 14852(T)).

A novel exopolysaccharide-producing bacterium, designated strain k53(T), was isolated from sediment from the Arabia Sea, Indian Ocean. The strain was Gram-negative, motile, strictly aerobic, oxidase-positive and catalase-positive, and required Na(+) for growth. Its major isoprenoid quinone was ubiquinone-8 (Q-8), and its cellular fatty acid profile mainly consisted of C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. The DNA G+C content was 43 mol%. 16S rRNA gene sequence analysis suggested that strain k53(T) is a member of the genus Pseudoalteromonas. Strain k53(T) exhibited close phylogenetic affinity to Pseudoalteromonas lipolytica LMEB 39(T) (98.0% 16S rRNA gene sequence similarity) and Pseudoalteromonas donghaensis HJ51(T) (97.3 %).The DNA-DNA reassociation values between strain k53(T) and P. lipolytica JCM 15903(T) and P. donghaensis LMG 24469(T) were 17 % and 12 %, respectively. Owing to the significant differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analysis based on the 16S rRNA gene sequence and DNA-DNA relatedness data, the isolate merits classification as a representative of a novel species, for which the name Pseudoalteromonas arabiensis is proposed. The type strain of this species is k53(T) ( = JCM 17292(T) = NCIMB 14688(T)).

Two novel exopolysaccharide-producing bacteria, strains S18K6(T) and S18K5, were isolated from Pacific Ocean sediment. The isolates were Gram-negative, motile, strictly aerobic chemoheterotrophic bacteria. The DNA G+C contents of strains S18K6(T) and S18K5 were 44.8 and 46.3 mol%, respectively. DNA-DNA relatedness between the two strains was 70 %. Major fatty acids were hexadecanoic acid (C(16 : 0)), hexadecenoic acid (C(16 : 1)omega7c) and octadecenoic acid (C(18 : 1)omega7c). 16S rRNA gene sequence, chemotaxonomic and morphological data indicated that these strains clearly belonged to the genus Glaciecola. Based on phenotypic properties and DNA-DNA hybridization data, strains S18K6(T) and S18K5 are considered to represent a novel species of the genus Glaciecola, for which the name Glaciecola chathamensis sp. nov. is proposed. The type strain is S18K6(T) (=JCM 13645(T)=NCIMB 14146(T)).

A central theme of the ongoing GEOTRACES program is to improve the understanding of processes occurring at ocean interfaces with continents, sediments, and ocean crust. In this context, we studied the distributions of Al, Mn, Fe, Co, Ni, Cu, Zn, Cd, and Pb around the Juan de Fuca Ridge (JdFR) in total dissolvable (td), dissolved (d), and labile particulate (lp) fractions, which represent a fraction in unfiltered samples, filtered samples through an AcroPak capsule filter, and the difference between td and d, respectively. Al and Fe were dominated by lp-species, while Ni, Zn, and Cd were dominated by d-species with undetectable amounts of lp-species. Major findings in this study are as follows: (1) The continental margin (CM) provided large sources of Al, Mn, Fe, and Co from the surface to ~2000 m in depth. The supply from CM caused high surface concentrations of dMn and dCo, a subsurface (100-300 m depth) maximum of dCo, and intermediate (500-2000 m depth) maxima of lpAl and lpFe. The supply of dFe from CM was ~10 times that from the high-temperature hydrothermal activity at station BD21, which is located at ~3 km from the Middle Valley venting site and ~ 200 km from Vancouver Island. (2) DPb was maximum at the top layer of North Pacific Intermediate Water, probably owing to isopycnal transport of anthropogenic Pb via advection of subducted surface waters. Although dCo and dPb had different sources in the upper water, they showed a strong linearity below 300 m ( r = 0.95, n = 38), indicating concurrent scavenging. (3) A high-temperature hydrothermal plume occurred at a depth of 2300 m at BD21, accounting for maxima of dAl, dMn, dFe, lpCu, and lpPb and a minimum of dCu. (4) Strong bottom maxima of lpAl, lpMn, lpFe, lpCo, and lpPb occurred above the abyssal plain at the western foot of the JdFR, indicating resuspension of sediments. However, bottom maxima of d-species were apparent only for dAl and dCu. [ABSTRACT FROM AUTHOR]