1. Methionine 58 is a key residue in the modulation of BmK scorpion toxin AGP-SYPU2 activity through in silico and in vivo studies.
- Author
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Cui Y, Wang T, Hao Z, Zhang J, and Zhao Y
- Subjects
- Amino Acid Sequence, Analgesics chemistry, Analgesics metabolism, Animals, Methionine metabolism, Scorpion Venoms chemistry, Scorpions chemistry, Scorpions metabolism
- Abstract
Protein dynamic networks play an important role in the regulation of many protein systems. Some residues that are far away from the interface between proteins and their targets have a critical role in modulating the activity of some scorpion toxins. Here, conservation analysis combined with an in vivo experiment has reveals that Met58 is a key residue of BmK scorpion toxin AGP-SYPU2 in the modulation of analgesic activity. Molecular dynamics simulations clearly reveal the conformational changes that allow the loop between the β2 and β3 sheets to be exposed on the toxin surface to interact with its targets. Our results emphasize specific roles for the residue Met58 in the NC domain and our work gives valuable information for further modification of scorpion toxins to obtain new analgesic peptides with enhanced activity. Communicated by Ramaswamy H. Sarma.
- Published
- 2022
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