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1. Novel polyclonal antibody-based rapid gold sandwich immunochromatographic strip for detecting the major royal jelly protein 1 (MRJP1) in honey.

Author

Yifan Zhang, Yong Chen, Yiting Cai, Zongyan Cui, Jinjie Zhang, Xiaohou Wang, and Lirong Shen

Subjects
Medicine, Science
Abstract

Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal. The cut-off value (sensitivity) of GSIS in detecting MRJP1 is 2.0 μg/mL in solution. Validation analysis with RJ, milk vetch honey, acacia honey and honey adulteration containing rice syrup and corn syrup with different ratios demonstrated that the GSIS could show consistent Test line (T line) when the test samples contain more than 30% pure honey or MRJP1 0.4 mg/g. The validation results by isotope ratio mass spectrometry on the same pure and all adulteration milk vetch honey samples showed the same information of GSIS test. The qualitative assay GSIS provided a valuable new way for honey authenticity and laid the foundation for the future application of GSIS with monoclonal antibodies in honey authentication.

Published
2019
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2. Novel polyclonal antibody-based rapid gold sandwich immunochromatographic strip for detecting the major royal jelly protein 1 (MRJP1) in honey

Author

Zongyan Cui, Xiaohou Wang, Jinjie Zhang, Li-rong Shen, Yong Chen, Yiting Cai, and Yifan Zhang

Subjects
Physiology, Future application, Metal Nanoparticles, 01 natural sciences, Biochemistry, Animal Products, Immune Physiology, Royal jelly, Medicine and Health Sciences, Food science, Enzyme-Linked Immunoassays, Flowering Plants, Immunoassay, Multidisciplinary, Milk Vetch, Immune System Proteins, biology, Chemistry, digestive, oral, and skin physiology, Acacia, food and beverages, Eukaryota, Agriculture, 04 agricultural and veterinary sciences, Honey, Bees, Hydrogen-Ion Concentration, Plants, 040401 food science, Body Fluids, Corn syrup, Laboratory Equipment, Test line, Milk, Experimental Organism Systems, behavior and behavior mechanisms, Medicine, Insect Proteins, Engineering and Technology, Anatomy, Research Article, endocrine system, food.ingredient, Science, Immunology, Equipment, Research and Analysis Methods, Antibodies, Beverages, 0404 agricultural biotechnology, food, Model Organisms, Plant and Algal Models, Animals, Grasses, Immunoassays, Centrifuges, Glycoproteins, Nutrition, 010401 analytical chemistry, fungi, Organisms, Biology and Life Sciences, Proteins, Honey samples, 0104 chemical sciences, Diet, Maize, Polyclonal antibodies, Food, biology.protein, Immunologic Techniques, Animal Studies, Gold, Rice
Abstract

Honey adulteration is becoming increasingly alarming incidents in food safety. Monitoring and detecting adulteration face greater challenges. Honey contains the major royal jelly proteins (MRJP) secreted by bee workers. To detect honey adulteration fast and accurately, a rapid gold sandwich immunochromatographic strip (GSIS) was developed based on two specific polyclonal antibodies (PoAbs) against the MRJP1, the most abundant protein of all MRJPs. We determined the best of pH value (pH 8.6) and PoAb SP-1 amount (5 μg/mL) in conjunction with colloidal. The cut-off value (sensitivity) of GSIS in detecting MRJP1 is 2.0 μg/mL in solution. Validation analysis with RJ, milk vetch honey, acacia honey and honey adulteration containing rice syrup and corn syrup with different ratios demonstrated that the GSIS could show consistent Test line (T line) when the test samples contain more than 30% pure honey or MRJP1 0.4 mg/g. The validation results by isotope ratio mass spectrometry on the same pure and all adulteration milk vetch honey samples showed the same information of GSIS test. The qualitative assay GSIS provided a valuable new way for honey authenticity and laid the foundation for the future application of GSIS with monoclonal antibodies in honey authentication.

Published
2018

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