1. Detection of SARS-CoV-2 spike protein D614G mutation using μTGGE.
- Author
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Juma KM, Morimoto K, Sharma V, Sharma K, Biyani R, Biyani M, Takita T, and Yasukawa K
- Subjects
- Denaturing Gradient Gel Electrophoresis, Mutation, SARS-CoV-2 genetics, Spike Glycoprotein, Coronavirus genetics
- Abstract
Background: The accurate and expeditious detection of SARS-CoV-2 mutations is critical for monitoring viral evolution, assessing its impact on transmission, virulence, and vaccine efficacy, and formulating public health interventions. In this study, a detection system utilizing micro temperature gradient gel electrophoresis (μTGGE) was developed for the identification of the D614 and G614 variants of the SARS-CoV-2 spike protein., Methods: The in vitro synthesized D614 and G614 gene fragments of the SARS-CoV-2 spike protein were amplified via polymerase chain reaction and subjected to μTGGE analysis., Results: The migration patterns exhibited by the D614 and G614 variants on the polyacrylamide gel were distinctly dissimilar and readily discernible by μTGGE. In particular, the mid-melting pattern of D614 was shorter than that of G614., Conclusions: Our results demonstrate the capability of μTGGE for the rapid, precise, and cost-effective detection of SARS-CoV-2 spike protein D614 and G614 variants without the need for sequencing. Therefore, this approach holds considerable potential for use in point-of-care mutation assays for SARS-CoV-2 and other pathogens., (© 2024. The Author(s), under exclusive licence to Springer Nature B.V.) more...
- Published
- 2024
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