1. An Adaptable Phospholipid Membrane Mimetic System for Solution NMR Studies of Membrane Proteins
- Author
-
Chien, Chih-Ta Henry, Helfinger, Lukas R, Bostock, Mark J, Solt, Andras, Tan, Yi Lei, Nietlispach, Daniel, Nietlispach, Daniel [0000-0003-4364-9291], and Apollo - University of Cambridge Repository
- Subjects
Magnetic Resonance Spectroscopy ,Biomimetics ,Membrane Proteins ,Nanoparticles ,Membranes, Artificial ,Hydrogen-Ion Concentration ,Dimyristoylphosphatidylcholine ,Phospholipids ,Saposins ,Receptors, G-Protein-Coupled - Abstract
Based on the saposin-A (SapA) scaffold protein, we demonstrate the suitability of a size-adaptable phospholipid membrane-mimetic system for solution NMR studies of membrane proteins (MPs) under close-to-native conditions. The Salipro nanoparticle size can be tuned over a wide pH range by adjusting the saposin-to-lipid stoichiometry, enabling maintenance of sufficiently high amounts of phospholipid in the Salipro nanoparticle to mimic a realistic membrane environment while controlling the overall size to enable solution NMR for a range of MPs. Three representative MPs, including one G-protein-coupled receptor, were successfully incorporated into SapA-dimyristoylphosphatidylcholine nanoparticles and studied by solution NMR spectroscopy.
- Published
- 2017