1. Bioactive spirostanol saponins from the rhizome of Tupistra chinensis.
- Author
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Xiang L, Wang Y, Yi X, Zheng G, and He X
- Subjects
- Animals, Antineoplastic Agents isolation & purification, Cell Proliferation drug effects, Humans, K562 Cells, Lipopolysaccharides pharmacology, Macrophages drug effects, Macrophages metabolism, Mice, Nitric Oxide biosynthesis, RAW 264.7 Cells, Saponins isolation & purification, Antineoplastic Agents chemistry, Antineoplastic Agents pharmacology, Asparagaceae chemistry, Rhizome chemistry, Saponins chemistry, Saponins pharmacology, Spirostans chemistry
- Abstract
Phytochemical investigations of the rhizome of Tupistra chinensis led to the isolation of six new spirostanol saponins, one new spirostanol, along with eight known spirostanols. Their chemical structures were elucidated on the basis of spectroscopic and chemical methods, including IR, NMR, MS, and GC analyses. The antiproliferative effects against five human cancer cell lines were assayed for all the isolated compounds. Compounds 8, 12 and 15 showed potent cytotoxic activities against K562 cells. The isolated compounds were evaluated for their inhibitory activities on nitric oxide (NO) production induced by lipopolysaccharide in a macrophage cell line RAW 264.7. Compounds 2 and 12 showed significant inhibition on NO production with IC50 values of 16.1±1.8 and 13.5±1.2 μM, respectively., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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