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In this study, five novel triterpenes were isolated from hydrolyzate of total saponins from Gynostemma pentaphyllum and identified as gypensapogenin H (1), gypensapogenin I (2), gypensapogenin L (3), gypensapogenin J (4) and gypensapogenin K (5), three of which (1-3) possess unprecedented ring A. All the isolated compounds were evaluated for cytotoxic activities in five cell lines and all the tested compounds showed significant anti-cancer activities against a series of human cancer cell lines, while having much weaker effect on the growth of normal cell. Among them, compound 1 showed strong inhibition toward MCF-7 human breast cancer cells (IC50 values 6.85 μM). Further mechanistic study demonstrated that compound 1 significantly induced MCF-7 cell apoptosis. Our results indicated that compound 1 may be a promising lead agent for further study., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

Protein tyrosine phosphatase 1B (PTP1B) is an important factor in non-insulin-dependent diabetes mellitus (type-2 diabetes), and a promising target for treatment of diabetes and obesity. Therefore, the aim of this study is to investigate the inhibitory activities of constituents (three new together with twelve known triterpenes compounds) isolated from the hydrolyzate of total saponins from Gynostemma pentaphyllum. Their structures were accomplished mainly base on the spectroscopic methods, and then were further confirmed by X-ray crystal diffraction. All the compounds were evaluated for inhibitory activity against PTP1B. Current data suggested that the compounds 1, 3, 12, 13 and 14 were considered to be potential as antidiabetic agents, in which they could significantly inhibit the PTP1B enzyme activity in a dose-dependent manner., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

A new dammarane-type triterpene saponin was isolated from the aerial parts of Gynostemma pentaphyllum (Thunb.) Makino. Its structural elucidation was accomplished mainly on the basis of the interpretation of spectroscopic data, such as IR, HR-TOF-MS and NMR. Its cytotoxic activity was evaluated against one human cancer cell line HL-60 using MTT assay.

Four new dammarane-type triterpene saponins, 1-4, were isolated from the aerial parts of Gynostemma pentaphyllum (Thunb.) Makino. Their structural elucidations were accomplished mainly on the basis of spectroscopic methods, such as IR, HR-TOF-MS, and NMR. Compounds 1-4 showed moderate cytotoxic activities against cancer cell lines HL-60, Colon205, and Du145 in vitro.

Two novel noroleanane saponins, tubeimoside A ( 1) and tubeimoside B ( 2), and a new dammarane triterpene saponin, tubeimoside C ( 3), together with two known compounds, 4 and 5, were isolated from the bulbs of Bolbostemma paniculatum ( Maxim.) Franquet. Compound 4 was found in this genus for the first time. Based on spectroscopic methods, including IR, NMR (DEPT, COSY, HMQC, HMBC, and TOCSY), and MS experiments, and chemical reactions, the structures of the new compounds were elucidated as 3 β-[ β- D-glucopyranosyl-(1→2)- β- D-glucopyranosyloxy]-2 β,23-dihydroxy-28-norolean-12-en-16-one ( 1), 3 β-[ β- D-glucopyranosyl-(1→2)- β- D-glucopyranosyloxy]-2 β,23-dihydroxy-28-norolean-12-en-22-one ( 2), (3 β,7 β)-7,18,20-trihydroxydammar-24-en-3-yl 2- O- α- L-arabinopyranosyl- β- D-glucopyranoside ( 3). [ABSTRACT FROM AUTHOR]

isolation and structure determination of two novel noroleanane saponins, tubeimoside A (I) and regioisomeric tubeisomide B, and a new dammarane triterpene saponin, tubeimoside C (II), together with two known compounds [ABSTRACT FROM AUTHOR]