1. Gene expression during development and overexpression after Cercospora kikuchii and salicylic acid challenging indicate defensive roles of the soybean toxin.
- Author
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Arantes MR, Dias LP, Costa JH, Saraiva KDC, Morais JKS, Sousa DOB, Soares AA, Vasconcelos IM, and Oliveira JTA
- Subjects
- Cotyledon genetics, Cotyledon metabolism, Gene Expression, Gene Expression Regulation, Plant drug effects, Gene Expression Regulation, Plant genetics, Glycoproteins genetics, Glycoproteins metabolism, Glycoproteins pharmacology, Plant Leaves drug effects, Plant Leaves genetics, Plant Leaves metabolism, Plant Leaves microbiology, Promoter Regions, Genetic, Seeds genetics, Seeds metabolism, Soybean Proteins genetics, Soybean Proteins metabolism, Soybean Proteins pharmacology, Glycine max genetics, Glycine max growth & development, Glycine max microbiology, Up-Regulation, Ascomycota drug effects, Ascomycota growth & development, Disease Resistance genetics, Glycoproteins physiology, Plant Diseases microbiology, Salicylic Acid pharmacology, Soybean Proteins physiology, Glycine max metabolism
- Abstract
Key Message: SBTX has defensive role against C. kikuchii, and therefore, its constituent genes SBTX17 and SBTX27 are promising candidates to engineer pathogen resistant plants. Soybean (Glycine max [L.] Merr.) is economically the most important legume crop in the world. Its productivity is strongly affected by fungal diseases, which reduce soybean production and seed quality and cause losses of billions of dollars worldwide. SBTX is a protein that apparently takes part in the defensive chemical arsenal of soybean against pathogens. This current study provides data that reinforce this hypothesis. Indeed, SBTX inhibited in vitro the mycelial growth of Cercospora kikuchii, it is constitutively located in the epidermal region of the soybean seed cotyledons, and it is exuded from mature imbibed seeds. Moreover, RT-qPCR analysis of the SBTX associated genes, SBTX17 and SBTX27, which encode for the 17 and 27 kDa polypeptide chains, showed that both genes are expressed in all studied plant tissues during the soybean development, with the highest levels found in the mature seeds and unifoliate leaves. In addition, to assess a local response of the soybean secondary leaves from 35-day-old plants, they were inoculated with C. kikuchii and treated with salicylic acid. It was verified using RT-qPCR that SBTX17 and SBTX27 genes overexpressed in leaves compared to controls. These findings strongly suggest that SBTX has defensive roles against C. kikuchii. Therefore, SBTX17 and SBTX27 genes are promising candidates to engineer pathogen resistant plants.
- Published
- 2020
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