1. A chimeric subunit of yeast transcription factor IIIC forms a subcomplex with tau95.
- Author
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Manaud N, Arrebola R, Buffin-Meyer B, Lefebvre O, Voss H, Riva M, Conesa C, and Sentenac A
- Subjects
- Amino Acid Sequence, DNA Polymerase III genetics, DNA, Fungal isolation & purification, DNA, Fungal metabolism, Gene Rearrangement, Molecular Sequence Data, Molecular Weight, Open Reading Frames, Protein Conformation, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins isolation & purification, Saccharomyces cerevisiae metabolism, Sequence Deletion, Transcription, Genetic, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae Proteins, Transcription Factors genetics, Transcription Factors, TFIII
- Abstract
The multisubunit yeast transcription factor IIIC (TFIIIC) is a multifunctional protein required for promoter recognition, transcription factor IIIB recruitment, and chromatin antirepression. We report the isolation and characterization of TFC7, an essential gene encoding the 55-kDa polypeptide, tau55, present in affinity-purified TFIIIC. tau55 is a chimeric protein generated by an ancient chromosomal rearrangement. Its C-terminal half is essential for cell viability and sufficient to ensure TFIIIC function in DNA binding and transcription assays. The N-terminal half is nonessential and highly similar to a putative yeast protein encoded on another chromosome and to a cyanobacterial protein of unknown function. Partial deletions of the N-terminal domain impaired tau55 function at a high temperature or in media containing glycerol or ethanol, suggesting a link between PolIII transcription and metabolic pathways. Interestingly, tau55 was found, together with TFIIIC subunit tau95, in a protein complex which was distinct from TFIIIC and which may play a role in the regulation of PolIII transcription, possibly in relation to cell metabolism.
- Published
- 1998
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