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The article presents the study results of soybean in the Ryazan district of the Ryazan region in 2013-2015. The paper considers the effect of biologically active preparations based on nanoparticles of iron, cobalt and copper on such physiological, biochemical and productive indexes of soybean as field germination, plant and root height, plant and root weight, leaf area, elements of yield structure, productivity and chemical composition. The study showed that the bio-preparation based on nanoparticles of copper increased the plant height on 9.7% in the branching phase and the weight of vegetative part of the plants on 26.4%. The application of the bio-preparation based on nanoparticles of cobalt gave the best result. The root weight of the plants during a vegetation period increased on 17.7%, productivity of soybean grain increased on 20% and content of protein in grain increased on 4.9% compared with the control variety. The field trials of efficiency of preparations based on nanoparticles of iron, cobalt and copper in the cultivation technology of the soybean in the Central Non-Black earth part of RF showed that nanoparticles of cobalt possess the best efficiency that promotes the further study and introduction of growth regulator into production.