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The carcasses of pigs belonging to dilTcrcnt age groups were brought from three farms in Thrissur district to the Department of Veterinary Pathology, College of Veterinary and Animal Sciences, Mannuthy for post mortem examination. Classical swine fever (CSF) was suspected based on history, clinical symptoms narrated by the owners and lesions noted on post mortem examination. In all the cases, owners reported heavy morality among the piglets. The post mortem lesions such as haemorrhages in lymph nodes, diffuse white focal areas in kidneys, haemorrhagic gastritis, diphtheritic membrane formation in intestine, degenerated and necrotic areas on liver and pulmonary emphysema with marbled appearance of lungs of varying degrees were noticed. In the fourth case, owner reported a heavy mortality and production loss among pigs in his farm and brought a live piglet of about two-month-old to the Department of Veterinary Microbiology for disease investigation. Erythematous patches was observed on the skin of affected piglet which is indicative of CSF. The piglet showed enlarged lymph nodes, pneumonic changes in lungs, hepatosis and cardiac hypertrophy on post-mortem inspection. All four cases were diagnosed as CSF by a polymerase chain reaction (PCR) targeting the E2gene of CSF virus which revealed an amplicon size of 308 bp. [ABSTRACT FROM AUTHOR]

Three ailing broilers of age 3 weeks, 5 weeks and 9 weeks respectively were presented to the Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy from three different private farms in Thrissur district for disease investigation. Owners reported a high mortality rate among the flocks and the birds were showing symptoms such as anorexia, depression, ruffled feathers, white watery diarrhoea and death. On post mortem examination, enlargement of bursa suggestive of infectious bursal disease (IBD) was noticed. The further confirmation of the cases were done with a reverse transcriptase polymerase chain reaction (RT-PCR) targeting the VP2 gene of Infectious bursal disease virus (IBDV), which revealed an amplicon size of 480 bp, specific for VP2 gene.