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The aim of this study was to determine the total phenolic content (TPC) of royal jelly harvested in the municipality of Konjic, by a spectrophotometric method using the Folin-Ciocalteu reagent. A gallic acid (GA) solution was used as a standard. All measurements were performed in triplicate at the wavelength of 764 nm The TPC was expressed in mg of GA equivalent (GAE) per 100 g royal jelly sample. The mean value of TPC in 12 samples of royal jelly was 411.72±8.83 mg GAE/100 g. The mean TPC in the 11 polyfloral samples of royal jelly (407.87±9.46 mg GAE/100 g) was slightly higher than mean TPC (369.53±11.65 mg GAE/100 g) in the monofloral sample (only one sample, measured in triplicate). The selected and applied spectrophotometric method proved to be an efficient and simple method for the quantitative determination of TPC in samples of royal jelly. [ABSTRACT FROM AUTHOR]

The aim of this study was to quantify the total protein content (TPrC) in samples of royal jelly harvested in the municipality of Konjic, using an UV spectrophotometric method. Bovine serum albumine was used as a protein standard to conctruct the calibration curve. The value of TPrC in polyfloral samples of royal jelly was 21.21±1.18%, while monofloral samples of royal jelly (Acacia flowers) showed the TPrC of 20.23±0.24%. Compared to literature data, this method produced higher values of TPrC in the complex matrices such as royal jelly. The employed spectrophotometric method proved to be very simple and convenient for the quantification of total proteins. [ABSTRACT FROM AUTHOR]