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A growing body of research supports an association between periapical inflammation and an increased risk of developing systemic diseases. There is currently no scientific evidence to support a causal effect of inflammation on the onset of insulin resistance (IR) in patients with apical periodontitis (AP). The aim of this in vitro study was to evaluate any association between AP and levels of serum inflammatory factors potentially associated with the onset of IR, and to investigate the effect of root canal treatment (RCT) on these systemic inflammation markers and on the response in vitro to insulin. A total of 27 control subjects and 27 patients with AP were enrolled. Patients with AP underwent RCT and were followed-up 6 and 12 months post-treatment. Enzyme-linked immunosorbent assays were used to evaluate serum levels of proinflammatory cytokines interleukin (IL)-1, IL-6, IL-8, and tumor necrosis factor (TNF)-α. The response in vitro to insulin was assessed by measuring glucose consumption in a human pancreatic epithelioid carcinoma cell line treated with sera from healthy and AP subjects. At baseline AP was associated with significant higher levels of IL-1, IL-6, and IL-8 in the serum of untreated (AP) patients vs controls (P <.001). Glucose consumption decreased in pancreatic cells incubated with baseline serum from patients with AP, in a manner proportional to total cytokines amount. Notably, endodontic treatment was associated with reduced levels of cytokines (P <.001) and improved response to insulin in AP group (P <.001). Our findings suggest that AP may promote inflammatory-driven IR in an in vitro model, and that RCT may ameliorate inflammatory mediators in vivo and the cellular response to insulin in vitro. [ABSTRACT FROM AUTHOR]

Background: Endodontic ledge (EL) formation is the most common complication of endodontic treatment. Although various etiological factors have been identified, canal curvature is the most significant variable correlated with EL formation. The aim of this micro-computed tomographic (micro-CT) study was to evaluate EL position in the mesial canals of the lower molars in relation to the degree of canal curvature. Methods: Forty intact mandibular first molars with independent mesial canals with 20°–40° primary mesio-distal curvature, 10°–30° buccal-lingual canal curvature and 4 < r ≤ 8 mm main curvature radius were selected. Working length was measured with a K-File #10 and a high resolution pre-operative micro-CT analysis was performed. Ledges were created at the point of maximum canal curvature using stainless steel K-Files #30–35, alternating irrigation with 5% NaOCl and 10% EDTA. A post-operative high-resolution micro-CT analysis was then completed. Pre- and post-operative images were analyzed. The angle (α) formed between the vector passing through the geometric center of the EL and the center of the original canal lumen and the line joining the centers of the mesio-buccal and mesio-lingual canal orifices was calculated, and a descriptive statistical analysis was achieved. The α angle values were analyzed in relation to canal curvature using Kruskal-Wallis and post hoc Dunn's tests. The level of significance was set at P < 0.05. Results: The α angles appeared inversely proportional to canal curvatures in the buccal-lingual and mesio-distal projections. The mean α angle was 36.4° (standard deviation 10.64; 95% confidence interval 34.1–40.9). Conclusion: Within the limitations of this study, endodontic ledges develop in the opposite direction to the three-dimensional canal curvature and their position is influenced by the degree of curvature. Clinically, the α angle values may be related to the recommended direction to manage endodontic ledges. [ABSTRACT FROM AUTHOR]

The aim of this study was to compare the shaping ability of a modified ProTaper Next technique (PTNm) with that of TruNatomy (TN) in lower molars mesial curved canals using micro-computed tomography (Micro-CT). Sixty mesial canals of first mandibular molars were randomly assigned between two groups (n = 30). After canal scouting with K-File #10, glide path and shaping were carried out with TN or PTNm systems. The PTNm sequence consists of ProGlider, followed by ProTaper Next X1 and apical finishing with NiTiFlex #25 up to working length (WL) to ensure adequate apical cleaning. Samples were scanned using micro-CT and pre- and post-shaping volumes were matched to analyse geometric parameters: the volume of removed dentin; the difference of canal surface; centroid shift, minimum and maximum root canal diameters; cross-sectional areas; the ratio of diameter ratios (RDR) and the ratio of cross-sectional areas (RA). Measurements were assessed 2 mm from the apex and in relation to the middle and coronal root canal thirds. Data were analysed using ANOVA (p < 0.05). No statistically significant differences were found between the groups for any parameter at each level of analysis, except for RA at the coronal level (p = 0.037). The PTNm system showed the tendency to enlarge more in the coronal portion with a lower centroid shift at apical level compared with TN sequence (p > 0.05). Both PTNm and TN sequences demonstrated similar maintenance of original anatomy during the shaping of lower molar mesial curved canals. [ABSTRACT FROM AUTHOR]

Featured Application: Root canal shaping techniques influence patients' postoperative quality of life after a primary root canal treatment. The introduction of more flexible reciprocating instruments with different alloy and geometry could lead to a general improvement of the postoperative symptoms. Patient-centered outcomes are crucial to evaluate the quality of the root canal treatment. Postoperative pain is a frequent complication of root canal treatment. It could worse patients' quality of life (QoL) and it may be associated to several factors, including the shaping technique. The aim of the study was to compare the impact of WaveOne Gold (WOG) and WaveOne Classic (WOC) reciprocating instrumentation on postoperative QoL after single-visit primary root canal treatment. Healthy subjects with pulp necrosis on multirooted teeth were observed. Canal shaping was performed with WaveOne Gold Primary (n = 25) or WaveOne Classic Primary (n = 29) and canal filling was completed with a carrier-based technique. Mean and maximum scores for postoperative pain were assessed through a Visual Analogue Scale (VAS) and QoL indicators were evaluated with a self-assessment questionnaire based on a Likert scale. Postoperative pain curves were similar in both groups (mean pain p = 0.43; maximum pain p = 0.27) and quality of life indicators showed no significant differences (p > 0.05). There was a more favourable trend of QoL values in the WOG group, reaching statistical significance on day six posttreatment (p = 0.021). Within the limitations of the study, reciprocating instrumentation may have an impact on patients' QoL, but the innovative geometrical and alloy properties of the WaveOne Gold seemed to induce a faster resolution of the postoperative symptoms. [ABSTRACT FROM AUTHOR]

Featured Application: The influence of brushing motion on root canal anatomy preservation after shaping with NiTi single file reciprocating instruments was evaluated. The brushing movement may accentuate canal transportation especially in the coronal third. Brushing motion has been proposed for endodontic single-file reciprocating systems to eliminate coronal interferences, but it may hesitate in a less conservative root canal shaping. The aim of the study was to compare the maintenance of the original root canal anatomy using the WaveOne Gold (WOG) technique with or without a brushing motion. Sixty extracted human mandibular first molars were selected. Manual canal scouting and mechanical glide path were performed. Samples were randomized into two groups (n = 30): WOG Primary was used to shape the mesio-lingual canals without (NB group) or with (B group) an intentional brushing motion. Specimens were scanned with micro-computed tomography (micro-CT) before and after instrumentation in order to match the volumes. Increases in canal volume and surface area were measured. Centroid shift and thickness of removed dentine from the inner curvature were assessed at the furcation, 1.5 mm and 3 mm apically from the furcation, and in relation to the point of maximum curvature. Data were analyzed by one-way ANOVA and post-hoc Student–Newmann–Keuls tests (p < 0.05). No brushing technique resulted in being significantly associated with a better maintenance of the canal anatomy, especially in the coronal third (p < 0.05). When using the WOG system, a no-brushing technique resulted in less invasive shaping, reducing the risk of stripping. [ABSTRACT FROM AUTHOR]

Featured Application: Finite element analysis is proposed as a reliable tool for the evaluation of the shaping ability of NiTi rotary instruments during an early design phase. This method could significantly reduce instrument development time and costs without the use of prototypes. The aim of this study was to evaluate the contact pressure distribution of two different nickel-titanium (NiTi) endodontic rotary instruments against the root canal walls and to virtually predict their centering ability during shaping with finite element analysis (FEA). Resin blocks simulating root canals were used. One was shaped with ProGlider and ProTaper Next (PTN) X1-X2 and one with ScoutRace and BioRace (BR) 1, 2 and 3. Both resin blocks were virtually replicated with computer-aided design (CAD) software. The endodontic instruments ProTaper Next (PTN) X2 and BioRace BR3 were also replicated with CAD. The NiTi instruments and the shaped blocks geometries were discretized and exported for FEA. The instrument rotation in the root canals was simulated. The finite element simulation was performed by applying an insertion and extraction force of 2.5 N with a constant rotational speed (300 rpm). To highlight possible differences between pressure distributions against the root canal portions outside and inside the canal curvature, the parameter Var was originally defined. Var values were systematically lower for PTN X2, revealing a better centering ability. FEA proved effective for the virtual prediction of the centering ability of NiTi instruments during an early design phase without the use of prototypes. [ABSTRACT FROM AUTHOR]

Introduction Contracted endodontic cavities (CECs) have developed from the concept of minimally invasive dentistry and provide an alternative to traditional endodontic cavities (TECs). They have been designed in an effort to preserve the mechanical stability of teeth. The contracted cavity design preserves more of the dentin but may influence the geometric shaping parameters. The aim of this micro–computed tomographic study was to evaluate the influence of contracted endodontic cavities on the preservation of the original root canal anatomy after shaping with nickel-titanium rotary instruments. Methods Thirty extracted human mandibular molars with fully formed apices and independent mesial canals were randomly assigned to group 1 (TEC) and group 2 (CEC). Each group was shaped using ProGlider (Dentsply Maillefer, Ballaigues, Switzerland) and WaveOne Gold (Dentsply Maillefer). Irrigation was performed with 10% EDTA and 5% sodium hypochlorite. Samples were scanned before and after canal shaping to match canal volumes (SkyScan; Bruker microCT, Kontich, Belgium [100 kV, 100 μA, and 15-μm resolution]), and images were analyzed to evaluate canal volumes, surface areas, and centroid shift on cross sections at −1 mm and −3 mm from the apex. Results TECs showed a greater preservation of the original root canal anatomy with less apical transportation than CECs, possibly because of the absence of coronal interferences and, therefore, fewer pecking motions required to complete instrumentation. Conclusions Within the limitations of this study, TECs may lead to a better preservation of the original canal anatomy during shaping compared with CECs, particularly at the apical level. [ABSTRACT FROM AUTHOR]

The aim of this in-vitro study was to evaluate the bond strength of fiber posts cemented in a root canal filled using various root-canal obturation techniques. A total of 33 monoradicular samples, treated endodontically, were randomly assigned to three groups according to the root-canal obturation technique: group 1, continuous-wave technique; group 2, plastic-obturator-core technique; and group 3, cross-linked gutta-percha obturator-core technique. Fiber posts were luted in each sample and each was sectioned perpendicular to the post axis. The push-out test was performed using a universal machine and the maximum failure load was recorded in MPa mm−2. Several samples were randomly chosen for scanning electron microscopy evaluation. The mean debris and dentinal tubule-opening scores were calculated separately in the coronal and apical portions. Bond strength was significantly higher in group 1 than in groups 2 and 3. Debris scores were significantly higher in the apical portion of groups 2 and 3 than in group 1. Within the limitations of this study it can be affirmed that thermoplasticized alpha gutta-percha seemed to worsen the cleaning of post-space walls and hence reduced fiber-post bond strength. [ABSTRACT FROM AUTHOR]

The sealing ability of gutta-percha/sealer root canal filing was compared to a new thermoplastic synthetic polymer-based obturation material (Resilon™), using a microleakage model and a new sequence detection assay One Cut Event AmplificatioN (OCEAN™). Eighty-eight extracted human teeth, shaped with K-Files and the ProTaper Technique, were randomly assigned to four groups (n = 22) and obturated in the apical 5 mm. Group R were obturated with the Resilon/Epiphany technique; group GP were obturated with gutta-percha and Zinc oxide eugenoe sealer; group RCH and GPCH received calcium hydroxide intracanal medication before being obturated. Sterilized specimens were inoculated with Enterococcus faecalis and incubated in sterile medium for 47 days. DNA extracted from the specimens was amplified by PCR and then identified by the OCEAN technique. Samples obturated with Resilon root canal filling material showed a greater number of microleakage events than the other groups (p = 0.036). Calcium hydroxide medication did not have a relevant impact on the quality of the apical seal (p = 0.044). [ABSTRACT FROM AUTHOR]

The aim of this study was to evaluate the ability of a PIPS (photon-induced photoacoustic streaming) Er:YAG laser to reduce the root canal system bacterial count in vivo in comparison to the traditional irrigation technique. The post-operative patients' quality of life (QoL) after endodontic therapy was evaluated through a questionnaire. Fifty-four patients affected by pulp necrosis with or without apical periodontitis biofilm disease were selected for endodontic treatment and randomly assigned to Group A (n = 27) with traditional irrigation and Group B (n = 27), with PIPS irrigation applied according to the protocol. Shaping was performed with ProGlider and ProTaper Next, and irrigation was performed with 5% NaOCl and 10% EDTA. Intracanal samples for culture tests were collected before and after irrigation. The microbiological analysis was evaluated by the Kolmogorov–Smirnov normality and Mann–Whitney tests (p < 0.05). A self-assessment questionnaire was used to evaluate the QoL during the 7 days after treatment; differences were analysed with Student's t-test. Irrigation with the PIPS device was significantly effective in reducing bacterial counts, which were higher for facultative than obligate anaerobic strains, particularly for Gram-negative bacteria, without statistical significance (p > 0.05). There were no significant differences among the QoL indicators, except for the maximum pain (p = 0.02), eating difficulty (p = 0.03) and difficulty performing daily functions (p = 0.02) in the first few days post-treatment. PIPS may represent an aid to root canal disinfection not affecting the patients' QoL, particularly for the first day after treatment. [ABSTRACT FROM AUTHOR]