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Your search keyword '"Tiruchinapalli, Dhanrajan M."' showing total 2 results
Start Over Author "Tiruchinapalli, Dhanrajan M." Topic rna-binding proteins
2 results on '"Tiruchinapalli, Dhanrajan M."'

1. Activity-dependent expression of ELAV/Hu RBPs and neuronal mRNAs in seizure and cocaine brain.

Author

Tiruchinapalli DM, Caron MG, and Keene JD

Subjects
Analysis of Variance, Animals, Antigens, Surface metabolism, Brain metabolism, Brain pathology, Dendrites metabolism, Disease Models, Animal, Dopamine Plasma Membrane Transport Proteins deficiency, ELAV Proteins metabolism, ELAV-Like Protein 1, Electrophoretic Mobility Shift Assay methods, Fragile X Mental Retardation Protein metabolism, Gene Expression Regulation drug effects, Immunoprecipitation methods, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Neurons cytology, Pilocarpine, RNA-Binding Proteins metabolism, Rats, Rats, Sprague-Dawley, Signal Transduction drug effects, Status Epilepticus chemically induced, Antigens, Surface genetics, Cocaine pharmacology, Dopamine Uptake Inhibitors pharmacology, ELAV Proteins genetics, Neurons metabolism, RNA, Messenger metabolism, RNA-Binding Proteins genetics, Status Epilepticus pathology
Abstract

Growing evidence indicates that both seizure (glutamate) and cocaine (dopamine) treatment modulate synaptic plasticity within the mesolimbic region of the CNS. Activation of glutamatergic neurons depends on the localized translation of neuronal mRNA products involved in modulating synaptic plasticity. In this study, we demonstrate the dendritic localization of HuR and HuD RNA-binding proteins (RBPs) and their association with neuronal mRNAs following these two paradigms of seizure and cocaine treatment. Both the ubiquitously expressed HuR and neuronal HuD RBPs were detected in different regions as well as within dendrites of the brain and in dissociated neurons. Quantitative analysis revealed an increase in HuR, HuD and p-glycogen synthase kinase 3beta (GSK3beta) protein levels as well as neuronal mRNAs encoding Homer, CaMKIIalpha, vascular early response gene, GAP-43, neuritin, and neuroligin protein products following either seizure or cocaine treatment. Inhibition of the Akt/GSK3beta signaling pathway by acute or chronic LiCl treatment revealed changes in HuR, HuD, pGSK3beta, p-Akt, and beta-catenin protein levels. In addition, a genetically engineered hyperdopaminergic mouse model (dopamine transporter knockout) revealed decreased expression of HuR protein levels, but no significant change was observed in HuD or fragile-X mental retardation protein RBPs. Finally, our data suggest that HuR and HuD RBPs potentially interact directly with neuronal mRNAs important for differentiation and synaptic plasticity.

Published
2008
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2. Activity-dependent trafficking and dynamic localization of zipcode binding protein 1 and beta-actin mRNA in dendrites and spines of hippocampal neurons.

Author

Tiruchinapalli DM, Oleynikov Y, Kelic S, Shenoy SM, Hartley A, Stanton PK, Singer RH, and Bassell GJ

Subjects
Actins biosynthesis, Actins genetics, Animals, Astrocytes cytology, Cells, Cultured, Coculture Techniques, Cytoplasmic Granules metabolism, Cytoplasmic Granules ultrastructure, Dendrites metabolism, Excitatory Amino Acid Antagonists pharmacology, Green Fluorescent Proteins, Hippocampus cytology, Hippocampus embryology, Internet, Luminescent Proteins genetics, Microscopy, Fluorescence methods, Neurons cytology, Potassium Chloride pharmacology, Protein Transport physiology, RNA, Messenger metabolism, RNA-Binding Proteins genetics, Rats, Receptors, N-Methyl-D-Aspartate antagonists & inhibitors, Receptors, N-Methyl-D-Aspartate metabolism, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Video Recording, Actins metabolism, Cell Surface Extensions metabolism, Hippocampus metabolism, Neurons metabolism, RNA-Binding Proteins metabolism
Abstract

RNA binding proteins may be important mediators of the activity-dependent transport of mRNAs to dendritic spines of activated synapses. We used fluorescence microscopy and digital imaging techniques applied to both fixed and live cultured hippocampal neurons to visualize the localization of the mRNA binding protein, zipcode binding protein 1 (ZBP1), and its dynamic movements in response to KCl-induced depolarization at high spatial and temporal resolution. With the use of immunofluorescence, image deconvolution, and three-dimensional reconstruction, ZBP1 was localized in the form of granules that were distributed in dendrites, spines, and subsynaptic sites. KCl depolarization increased the dendritic localization of ZBP1 that was not attributed to an increase in ZBP1 expression. Live cell imaging of single cells before and after perfusion of KCl revealed the rapid and directed efflux of ZBP1 granules from the cell body into dendrites in a proximo-distal gradient. High-speed imaging of enhanced green fluorescence protein-ZBP1 granules revealed rapid anterograde and retrograde movements in dendrites as well as dynamic movements in dendritic spines. A population of ZBP1 granules colocalized with beta-actin mRNA, and their spatial association in dendrites was increased by KCl depolarization. The NMDA receptor antagonist AP-5 impaired the dendritic localization of ZBP1 and beta-actin mRNA and inhibited the KCl-induced transport of ZBP1. The activity-dependent trafficking of ZBP1 and its dynamic movements within dendritic spines provide new evidence to implicate RNA binding proteins as regulators of mRNA transport to activated synapses in response to synaptic activity.

Published
2003

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