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Your search keyword '"Shaoyan Liang"' showing total 2 results
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2 results on '"Shaoyan Liang"'

1. Netrin-1 inducing antiapoptotic effect of acute myeloid leukemia cells in a concentration-dependent manner through the Unc-5 netrin receptor B-focal adhesion kinase axis.

Author

Kainan Zhang, Xizhou An, Yao Zhu, Lan Huang, Xinyuan Yao, Xing Zeng, Shaoyan Liang, and Jie Yu

Subjects
ACUTE myeloid leukemia, MYELOID cells, FOCAL adhesion kinase, HEMATOLOGIC malignancies, RNA
Abstract

Acute myeloid leukemia (AML) is a hematological malignancy that commonly occurs in children. The prognosis of pediatric AML is relatively poor, thus threatening the patient's survival. The aberrant expression of the axon guidance factor, netrin-1, is observed in various types of malignancies, and it participates in the proliferation and apoptosis of tumor cells. Herein, we aimed to explore the role of netrin-1 in AML cells. Netrin-1 is highly expressed in AML patients. Proliferation and anti-apoptosis were observed in AML cells treated with netrin-1. The interaction between netrin-1 and Unc-5 netrin receptor B (UNC5B) was detected through coimmunoprecipitation, and UNC5B ribonucleic acid interference restrained the influence of netrin-1 on the AML cells. The phosphorylation of focal adhesion kinaseprotein kinase B (FAK-Akt) was upregulated in AML cells treated with netrin-1. Both FAK and Akt inhibitors abrogated the effects of netrin-1 on the proliferation and apoptosis of AML cells. In conclusion, netrin-1 could promote the growth and reduce the apoptosis of AML cells in a concentration-dependent manner, and that these effects were mediated by activating the FAK-Akt signaling pathway via the UNC5B. [ABSTRACT FROM AUTHOR]

Published
2023
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2. miRNA Profiling Reveals Dysregulation of RET and RET-Regulating Pathways in Hirschsprung's Disease

Author

Yi Wang, Shaoyan Liang, Zhenhua Guo, Shiqi Wang, Xiaoqing Li, Huan Wu, Shuangshuang Li, and Xianqing Jin

Subjects
0301 basic medicine, Cell signaling, Microarrays, lcsh:Medicine, Signal transduction, Biochemistry, Pediatrics, Transcriptome, Phosphatidylinositol 3-Kinases, Cell Movement, Medicine and Health Sciences, lcsh:Science, Genetics, Regulation of gene expression, Multidisciplinary, Messenger RNA, Signaling cascades, Nucleic acids, Signal Filtering, Bioassays and Physiological Analysis, Proto-Oncogene Proteins c-ret, Child, Preschool, Engineering and Technology, Pediatric Gastroenterology, Mitogen-Activated Protein Kinases, Anatomy, DNA microarray, Research Article, Cell biology, MAPK signaling cascades, Colon, Gastroenterology and Hepatology, Biology, Research and Analysis Methods, 03 medical and health sciences, microRNA, Humans, Hirschsprung Disease, Non-coding RNA, PI3K/AKT/mTOR pathway, Cell Proliferation, Biology and life sciences, lcsh:R, Infant, Newborn, Infant, Gene regulation, Gastrointestinal Tract, MicroRNAs, 030104 developmental biology, Gene Expression Regulation, Signal Processing, Cancer research, Gene chip analysis, RNA, lcsh:Q, Gene expression, Proto-Oncogene Proteins c-akt, Digestive System
Abstract

Hirschsprung's disease (HSCR), the most common congenital malformation of the gut, is regulated by multiple signal transduction pathways. Several components of these pathways are important targets for microRNAs (miRNAs). Multiple miRNAs have been associated with the pathophysiology of HSCR, and serum miRNAs profiles of HSCR patients have been reported, but miRNA expression in HSCR colon tissue is almost completely unexplored. Using microarray technology, we screened colon tissue to detect miRNAs whose expression profiles were altered in HSCR and identify targets of differentially expressed miRNAs. Following filtering of low-intensity signals, data normalization, and volcano plot filtering, we identified 168 differentially expressed miRNAs (104 up-regulated and 64 down-regulated). Fifty of these mRNAs represent major targets of dysegulated miRNAs and may thus important roles in the pathophysiology of HSCR. Pathway analysis revealed that 7 of the miRNA targets encode proteins involved in regulation of cell proliferation and migration via RET and related signaling pathways (MAPK and PI3K/AKT). Our results identify miRNAs that play key roles in the pathophysiology of the complex multi-factorial disease HSCR.

Published
2016
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