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Your search keyword '"Arbieva Z"' showing total 3 results
Start Over Author "Arbieva Z" Topic rna, viral
3 results on '"Arbieva Z"'

1. [Formation of protein cross-links and breaks in the polynucleotide chain of tobacco mosaic virus RNA in situ after exposure to high-intensity pico-second laser UV-irradiation].

Author

Arbieva ZKh, Kalmykov PV, Dobrov EN, Esenaliev RO, and Morev PG

Subjects
Cross-Linking Reagents, Lasers, Macromolecular Substances, Polynucleotides analysis, RNA, Viral analysis, Tobacco Mosaic Virus analysis, Viral Proteins analysis, Polynucleotides radiation effects, RNA, Viral radiation effects, Tobacco Mosaic Virus radiation effects, Ultraviolet Rays, Viral Proteins radiation effects
Published
1987

2. UV laser induced RNA-protein crosslinks and RNA chain breaks in tobacco mosaic virus RNA in situ.

Author

Dobrov EN, Arbieva ZKh, Timofeeva EK, Esenaliev RO, Oraevsky AA, and Nikogosyan DN

Subjects
Lasers, Cross-Linking Reagents, Proteins radiation effects, RNA, Viral radiation effects, Tobacco Mosaic Virus radiation effects, Ultraviolet Rays
Abstract

The efficiency of RNA-protein crosslink and RNA chain break formation under nanosecond or picosecond UV-laser pulse irradiation of tobacco mosaic virus was determined. It was found that on high-intensity UV-laser irradiation the quantum yields of both reactions increase considerably as compared to the usual (low-intensity) UV-irradiation. The RNA-protein crosslink quantum yield was found to be 1.8 x 10(-5) and 1.2 x 10(-4) and that of RNA chain breaks 1.7 x 10(-4) and 8.9 x 10(-4) for nanosecond and picosecond irradiation, respectively.

Published
1989
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3. [Identification of a peptide of the membrane protein of tobacco mosaic virus, cross-linked with intraviral RNA under the effect of UV-radiation].

Author

Grebenshchikov NI, Arbieva ZKh, and Dobrov EN

Subjects
Capsid radiation effects, Chromatography, High Pressure Liquid, Hydrolysis, RNA, Viral radiation effects, Tobacco Mosaic Virus radiation effects, Ultraviolet Rays, Capsid metabolism, Peptides analysis, RNA, Viral metabolism, Tobacco Mosaic Virus metabolism
Abstract

As reported previously, UV-irradiation induces crosslinking between tobacco mosaic virus (TMV) coat protein molecules and intraviral RNA nucleotides. We have irradiated [3H]-uridine labeled TMV and isolated TMV coat protein subunits with the attached nucleotide label. These TMV protein subunits were hydrolyzed with trypsin. The tryptic peptides were separated by high-performance liquid chromatography and [3H]-labeled peptides were identified. The UV-irradiation of TMV was found to result in crosslinking to intraviral RNA of the T8 tryptic peptide (residues 93-112) of TMV coat protein.

Published
1989

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