1. Detection of immunoglobulin light-chain restriction in cutaneous B-cell lymphomas by ultrasensitive bright-field mRNA in situ hybridization.
- Author
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Minca EC, Wang H, Wang Z, Lanigan C, Billings SD, Luo Y, Tubbs RR, and Ma XJ
- Subjects
- B-Lymphocytes pathology, Flow Cytometry, Humans, Immunohistochemistry methods, Lymphoma, B-Cell immunology, Lymphoma, B-Cell metabolism, Lymphoma, B-Cell pathology, RNA, Messenger metabolism, Skin Neoplasms immunology, Skin Neoplasms pathology, Immunoglobulin Light Chains genetics, Immunoglobulin kappa-Chains genetics, Immunoglobulin lambda-Chains genetics, In Situ Hybridization methods, Lymphoma, B-Cell diagnosis, RNA, Messenger analysis, Skin Neoplasms diagnosis
- Abstract
Background: Detection of immunoglobulin light-chain restriction is important in the diagnosis of B-cell non-Hodgkin lymphoma (B-NHL). Flow-cytometry, commonly used to evaluate light-chain restriction, is impractical to be used in cutaneous specimens. Immunohistochemical and conventional chromogenic in situ hybridization (CISH) methods on formalin-fixed-paraffin-embedded (FFPE) tissue lack sufficient sensitivity to detect low-level light-chain expression in B-NHL without plasmacytic differentiation. Ultrasensitive bright-field mRNA-ISH (BRISH) for in situ light-chain detection in cutaneous B-NHL has been assessed., Design: Kappa/lambda mRNA was detected using two-color BRISH (RNAscope 2xPlex, Advanced Cell Diagnostics) on 27 FFPE skin biopsies and excisions from patients with available B-cell PCR clonality studies: 16 clonal B-cell lesions (6 follicle center lymphoma, 5 marginal zone lymphoma, 3 large B-cell lymphoma, and 2 other) and 11 non-clonal B-cell proliferations., Results: BRISH was successful in 15/16 clonal B-cell lesions and 11/11 non-clonal proliferations. Light-chain restriction was detected in 15/15 clonal lesions and in 1/11 non-clonal proliferations (96.1% overall concordance with clonality PCR). In 4/5 marginal zone lymphomas, light-chain restriction was detected as strong monotypic mRNA expression in a B-cell subset, consistent with plasmacytic differentiation., Conclusion: Ultrasensitive BRISH can successfully detect light-chain restriction in B-NHL from FFPE skin specimens and may be a useful adjunct ancillary tool in cases not resolved by CISH or immunohistochemical methods., (© 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2015
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