1. High methylation of the same promoter of lncRNA ZNF582-AS1 / ZNF582 promotes malignant progression of esophageal cancer.
- Author
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Li Z, Yan H, Wang B, Wang H, Chen A, Zhu T, Liu J, Yu G, and Kang M
- Subjects
- Humans, Animals, Cell Line, Tumor, Mice, Disease Progression, Cell Proliferation, Apoptosis genetics, Mice, Nude, Male, Female, Esophageal Neoplasms genetics, Esophageal Neoplasms pathology, DNA Methylation, RNA, Long Noncoding genetics, Promoter Regions, Genetic, Gene Expression Regulation, Neoplastic
- Abstract
Aim: This study aimed to investigate the functions of ZNF582-AS1 and ZNF582 in esophageal cancer (EC). Materials & methods: Bioinformatics analysis, qRT-PCR and western blot were used to analyze the expression levels. Biological functions were evaluated using cell-counting kit 8, colony formation, Transwell assays and flow cytometry. FISH was used to detect subcellular localization, and methylation-specific PCR determined gene methylation levels. Animal experiments validated the impact on tumor progression. Results: ZNF582-AS1 and ZNF582 were highly methylated and downregulated in EC. Overexpression of ZNF582-AS1 up-regulated the expression of ZNF582 , thereby inhibiting EC cell viability and metastasis, promoting apoptosis and inhibiting tumor growth. Conclusion: Low expression of ZNF582-AS1 / ZNF582 mediated by DNA hypermethylation facilitates the malignant progression of EC.
- Published
- 2024
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