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Quantitative microbial risk assessment (QMRA) can be used to evaluate health risks associated with recreational beach use. This study developed a site-specific risk assessment using a novel approach that combined quantitative PCR-based measurement of microbial source tracking (MST) genetic markers (human, dog, and gull fecal bacteria) with a QMRA analysis of potential pathogen risk. Water samples (n = 24) from two recreational beaches were collected and analyzed for MST markers as part of a broader Beach Exposure And Child Health Study that examined child behavior interactions with the beach environment. We report here the measurements of fecal bacteria MST markers in the environmental DNA extracts of those samples and a QMRA analysis of potential health risks utilizing the results from the MST measurements in the water samples. Human-specific Bacteroides was enumerated by the HF183 Taqman qPCR assay, gull-specific Catellicoccus was enumerated by the Gull2 qPCR assay, and dog-specific Bacteroides was enumerated by the DogBact qPCR assay. Derived reference pathogen doses, calculated from the MST marker concentrations detected in recreational waters, were used to estimate the risk of gastrointestinal illness for both children and adults. Dose-response equations were used to estimate the probability of the risk of infection (Pinf) per a swimming exposure event. Based on the QMRA simulations presented in this study, the GI risk from swimming or playing in water containing a mixture of human and non-human fecal sources appear to be primarily driven by the human fecal source. However, the estimated median GI health risk for both beaches never exceeded the U.S. EPA risk threshold of 32 illnesses per 1,000 recreation events. Our research suggests that utilizing QMRA together with MST can further extend our understanding of potential recreational bather risk by identifying the source contributing the greatest risk in a particular location, therefore informing beach management responses and decision-making. [ABSTRACT FROM AUTHOR]

Microbial contamination of private well systems continues to be a prominent drinking water concern, especially for areas impacted by floodwaters. Hurricane Harvey deposited nearly 60 inches of rain, resulting in extensive flooding throughout Houston, Texas, and neighboring counties. A sampling campaign to test private wells for fecal indicator bacteria was initiated in the weeks following flooding. Escherichia coli concentrations measured in wells were utilized in a quantitative microbial risk assessment to estimate the risk of infection for both drinking water and indirect ingestion exposure scenarios. Derived reference pathogen doses indicated that norovirus (1.60 × 10−4 to 8.32 × 10−5) and Cryptosporidium (2.37–7.80 × 10−6) posed the greatest health risk via drinking, with median health risk estimates exceeding the U.S. Environmental Protection Agency's modified daily risk threshold of 1 × 10−6 for a gastrointestinal infection. Bathing (1.78 × 10−6), showering (4.32 × 10−7), and food/dish washing (1.79 × 10−6) were also identified to be exposure pathways of health concern. A post-flood microbial risk assessment of private wells in the Gulf Coast has not previously been conducted. Estimating these health risks can provide scientifically supported guidance regarding which well water practices are safest, especially when well water quality is unknown. Developing this guidance is critical as coastal communities experience increased vulnerability to flooding. [ABSTRACT FROM AUTHOR]

Gastrointestinal (GI) illness risks associated with exposure to waters impacted by human and nonhuman fecal sources were estimated using quantitative microbial risk assessment (QMRA). Microbial source tracking (MST) results had identified Escherichia coli (E. coli) contributors to the waterbody as human and unidentified (10%), cattle and domestic animals (25%), and wildlife (65%) in a rural watershed. The illness risks associated with ingestion during recreation were calculated by assigning reference pathogens for each contributing source and using pathogen dose–response relationships. The risk of GI illness was calculated for a specific sampling site with a geometric mean of E. coli of 163 colony forming units (cfu) 100 mL−1, and the recreational standard of E. coli, 126 cfu 100 mL−1. While the most frequent sources of fecal indicator bacteria at the sampling site were nonhuman, the risk of illness from norovirus, the reference pathogen representing human waste, contributed the greatest risk to human health. This study serves as a preliminary review regarding the potential for incorporating results from library-dependent MST to inform a QMRA for recreational waters. The simulations indicated that identifying the sources contributing to the bacterial impairment is critical to estimate the human health risk associated with recreation in a waterbody. [ABSTRACT FROM AUTHOR]