Search

Beyaz peynir, a white brined cheese, was manufactured using different blends of camel chymosin (100, 75, 50, 25, and 0%) with calf chymosin and ripened for 90 d. The purpose of this study was to determine the best mixture of coagulant for Beyaz peynir, in terms of proteolysis, texture, and melting characteristics. The cheeses were evaluated in terms of chemical composition, levels of proteolysis, total free amino acids, texture, meltability, residual coagulant activity, microstructure, and sensory properties during 90 d of ripening. Differences in the gross chemical composition were statistically significant for all types of cheeses. Levels of proteolysis were highly dependent on the blends of the coagulants. Higher proteolysis was observed in cheeses that used a higher ratio of calf chymosin. Differences in urea-PAGE and peptide profiles of each cheese were observed as well. Meltability values proportionally increased with the higher increasing levels of calf chymosin in the blend formula. These coagulants had a slight effect on the microstructure of cheeses. The cheese made with camel chymosin had a harder texture than calf chymosin cheese, and hardness values of all cheese samples decreased during ripening. The cheeses with a high ratio of calf chymosin had higher residual enzyme activity than those made with camel chymosin. No significant difference in sensory properties was observed among the cheeses. In conclusion, cheeses made with a high level of calf chymosin had a higher level of proteolysis, residual coagulant activity, and meltability. The cheeses also had a softer texture than cheeses made with a high content of camel chymosin. Camel chymosin may be used as a coagulant alone if low or limited levels of proteolysis are desired in cheese.

Iranian Ultrafiltered White cheese was produced by using different blends of coagulants (100:0, 75:25, 50:50, 25:75 and 0:100; Rhizomucor miehei and camel chymosin, respectively) and ripened for 90 days. The effect of different combinations of these coagulants on chemical composition, proteolysis and residual coagulant activity of the cheeses were studied. The results showed that pH, fat-in-dry matter, salt-in-dry matter and protein contents of the cheeses were significantly influenced by type and concentration of the coagulants. The difference between proteolytic activities of the two coagulants resulted in different levels of proteolysis in the cheeses. A direct relationship was determined between using higher concentrations of R. miehei and increasing the hydrolysis of αs1-casein in the cheeses, during ripening. The residual coagulant activity was influenced by the type and concentration of the coagulant as well. In conclusion, R. miehei provided a higher level of proteolysis and residual coagulant activity compared with camel chymosin.

Adjunct cultures including lactobacilli play important role in cheese proteolysis and bioactivity including Angiotensin Converting Enzyme (ACE)-inhibition and antioxidant activity. Four species of lactobacilli was used in the manufacture of model cheese and these were salted at different levels and the resultant cheeses were ripened at different time and temperature to understand the effect of salting and ripening conditions. The effects of the use of various of lactobacilli (Lactobacillus casei, Lb. helveticus, Lb. delbrueckii ssp. bulgaricus and Lb. plantarum), salt concentrations (1, 2, 3, 4 and 5%), ripening times (1, 30, 60, 90 and 120 days) and temperatures (5, 10, 15 and 20 degrees C) were investigated. The experiments were designed to determine the optimal salt concentration, ripening times and temperatures for optimal proteolysis, the highest levels of ACE-inhibition and antioxidant activity. The highest level of proteolysis and ACE-inhibiting activity in the model cheese was observed when ripening temperatures were between 8 and 16 degrees C, salt concentration at 2.5-3.0% and with a ripening period of about 60 days. The use of each lactobacilli contributed to the proteolysis in different manner; for example, ACE-inhibiting activity and antioxidant activity in the model cheese.

Bouhezza is an Algerian cheese, which is ripened in a goat-skin bag, called Chekoua. The aim of the study was to determine the protein profiles and aromatics, which contribute to sensory properties of Bouhezza cheese. The chemical composition, proteolysis, and volatile profile have been carried out in cheese made from raw goat milk. The results showed that, the dry matter ranged from 23.07 to 51.95%, the fat-in-dry matter ranged from 10.58 to 31.77%, and the protein ranged from 28.27 to 42.09%. Water-soluble and 12% trichloroacetic acid-soluble nitrogen fractions decreased at the beginning of the ripening process and then they increased until the end of ripening. The reverse phase-high-performance liquid chromatography peptide profiles of the cheese showed the modifications occurred during the ripening process. The volatile compounds showed a diversity of odorous components which contribute to give to the cheese its particular organoleptic. There were 109 compounds identified in Bouhezza cheese. C...

The effects of partial substitution of goat's milk for sheep's milk, scalding and dry salting on the proteolysis of Urfa cheese were monitored for 240 days. For this reason, the contents of total nitrogen, water‐soluble nitrogen (WSN), and trichloroacetic acid‐soluble nitrogen (TCA‐SN) were explored as well as the chemical compositions of samples during ripening. Also, RP‐HPLC of pH 4.6‐soluble, 70% ethanol‐insoluble (EtOH‐i), and urea‐PAGE of pH 4.6‐insoluble nitrogen fractions of samples was determined. The results showed that the effects of partial substitution of goat's milk for sheep's milk on WSN and TCA‐SN were significant from sixth to eighth month of the ripening, respectively. Even though the effects of the milk type, dry salting, and scalding on the peptide profile were significant at the beginning of maturation, the peptide profile of cheeses was almost similar at the end of the storage. Furthermore, the proteolysis of Urfa cheese was limited due to dry salting during the ripening. PRACTICAL APPLICATIONS: The maturation of cheese is a complex biochemical process involving the hydrolysis of fat, breakdown of protein, and metabolism of lactose. Though the biochemical reactions during cheese ripening are not precisely understood, proteolysis is one of the most significant biochemical processes during maturation. It is known that the amino acids resulting from proteolysis are the precursors of flavor components in cheese. Milk itself is a significant source of proteolytic enzymes. And also, the curd scalding temperature and salt content of cheese affect the proteolytic activity during cheese ripening. However, limited proteolysis may be occurred during the cheese maturation due to dry salting and scalding process. Therefore, studies should focus on overcoming this problem. This study can help to overcome the limited proteolysis in the production of cheese. Also, results from the study may contribute to the industrial production of Urfa cheese.

The effect of aromatic herbs on the RP-HPLC peptide profile, angiotensin I-converting enzyme-inhibitory (ACE-i) activity, antioxidant capacity and volatile compounds in artisanal cheeses was investigated. Three representative artisanal cheeses (white brined, beaten and Kashkaval cheeses) were produced using aromatic herbs (Mentha longifolia, Origanum vulgare, Thymus longicaulis, Alium sativum, Petroselinum crispum, Capsicum annuum). Compared with control group, herbal cheeses (except white cheese with garlic and parsley) revealed a significant increase in antioxidant activity. The highest ACE-i activity was noticed in 60 days of ripening with a mean value of 98.72% in goat beaten cheese and 97.78% in Kashkaval cheese enriched with peppers. One hundred and one volatile compounds, mainly acids, esters and alcohols, were identified in significantly higher amounts in cheeses made with herbs and contributed to the development of the overall aroma perception.

Kashkaval is the most popular hard cheese in Macedonia and other countries of Balkan peninsula. The aim of this research is to assess the differences of heat treatments effect (60, 70 and 90 °C for 5 min) in several biochemical and technological characteristics of Kashkaval cheese. Proteolysis was observed to take place at a faster rate in the Kashkaval cheeses made using the lower heat treatment. The residual enzyme activity of cheeses averaged 67.7, 43.7 and 8.4% for the cheeses heated at 60, 70 and 90 °C, respectively. Acids and esters constituted the main chemical class of the cheeses during ripening (mean abundances of these were 57.1% and 26.8% w/w of total volatiles, respectively). The colour ( L ∗ ) and meltability values decreased significantly during ripening. In conclusion, powerful correlations were observed between extents of the heat treatment and levels of residual coagulant activity, breakdown of proteins and formation of volatiles.

Various blends of milk coagulants Rhizomucor miehei protease and camel chymosin were mixed at levels of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively and used in the manufacture of Iranian ultrafiltered (UF) White cheese. Effect of different blends of these coagulants on microstructure and rheological properties of Iranian UF White cheese were studied during 90 days of ripening. The results showed that both microstructure and rheology of the cheeses were influenced by type and concentration of these coagulant blends due to their different proteolytic activities. Use of high concentrations of camel chymosin resulted in a more compact protein network and firmer structure in the cheeses. Increasing levels of camel chymosin in the coagulant blends increased the storage ( G′ ) and loss ( G″ ) moduli and decreased loss tangent (δ) in the cheeses. In conclusion, increasing levels of camel chymosin provided less protein breakdown and more solid-like viscoelastic structure in UF cheeses.

The effects of addition of black cumin on the chemical composition, proteolysis, sensory properties and volatile profile of Erzincan Tulum cheese were analyzed. In general, the addition of black cumin caused an increase in total protein, ash and total nitrogen contents and water-soluble nitrogen concentrations when compared to black cumin-free (control) cheese. According to the results of the sensory analysis at 90 days of ripening, the general acceptability of samples was not found statistically different and cheese samples were scored with good marks (7–8 out of 9). Volatile compounds in the cheeses were identified by solid-phase microextraction combined with gas chromatography-mass spectrometry after 1, 30, 60, 90 and 180 days of ripening. A total of 94 volatiles were identified and these belong to different chemical groups such as alcohols (14), aldehydes (7), sulfur compounds (2), methyl ketones (8), esters (31), terpenes (12), carboxylic acid (10) and miscellaneous compounds (10). Esters, alcohols and terpenes were the most abundant classes of volatile components in Erzincan Tulum cheeses. In conclusion, the addition of black cumin positively contributed to the sensory scores and cheese proteolysis, and also enhanced the volatiles in cheese both qualitatively and quantitatively.

A mixed commercial starter culture containing Lactococcus lactis subps lactis and Lactococcus lactis subsp cremoris (Cheese A, control) was combined with three adjunct cultures including Lactobacillus casei (Cheese B), Lactobacillus plantarum (Cheese C) and Lactobacillus bulgaricus (Cheese D) in the manufacture of white brined cheese using goat's milk. Use of adjunct culture was not significantly affected pH and gross chemical composition except for nitrogenous compounds; however, use of adjunct cultures in cheese manufacture showed a potential increase in ACE-inhibitory and antioxidant activity parallel to the increase in water-soluble nitrogenous compounds until the 60th day of ripening. The highest antioxidant and ACE-inhibitory activity was detected in cheese D (62.55%) and cheese B (51.95%), respectively. All cheese samples showed almost similar peptide profile; however, quantitative differences were observed. In conclusion, use of adjunct cultures in white-brined goat-milk cheese manufacturing contribute to increase in ACE-inhibitory activity and antioxidant activity.

Three different strains of Penicillium roqueforti were used for the manufacture of mold‐ripened Civil cheese and these cheeses were ripened at 4 and 10 °C for 90 days. Sixty‐five volatile compounds were identified in the volatile fractions of the cheeses. The concentration of volatile compounds in cheeses was significantly affected ripening time temperature and by mold strains a lesser extent. 3‐Methyl‐1‐butanol, 2‐nonanol, methyl dodecanoate, butanoic acid, and decanoic acid were the most frequently identified volatiles. Tryptamine, 2‐phenylethylamine, putrescine, cadaverine, tyramine, spermidine, and spermine were also found. The most abundant biogenic amine was cadaverine. The formation of biogenic amines was significantly influenced by strain, ripening time, and temperature. The biogenic amine content was found to be between 2.41 and 228.94 μg/g cheese. Ripening temperature and ripening time significantly influenced the formation of volatile compounds and biogenic amines. PRACTICAL APPLICATIONS: Moldy Civil is a traditional cheese variety produced in the eastern Anatolian region of Turkey and it is protected by geographical indication by Turkish Patent and Trademark Office (TURKPATENT) by 2010. It is manufactured using skimmed milk which is spontaneously acidified with native lactic acid bacteria and also the addition of preacidified whey. Three strains of P. roqueforti was used in Moldy Civil cheese‐making and strains of the mold changed the volatile profiles and the levels of biogenic amines less than ripening time and temperature. Methyl ketones and alcohols were the dominant groups in volatiles, while cadaverine was found to be principal biogenic amine in Moldy Civil cheese. Use of P. roqueforti strains contributed to the formation of the volatile fraction of the cheese; however, the level of biogenic amines changed by P. roqueforti strains a lesser extent.

PubMedID: 26119429 Iranian White cheese was manufactured from ultrafiltered cows' milk using different concentrations of salt consisting of 1, 2·5, 4% and salt free. Chemical composition, proteolysis, counts for lactic acid bacteria and sensory evaluation were examined during 90 d of ripening. It was found that the use of different salt concentrations significantly influenced all chemical composition, proteolysis, total number of lactic acid bacteria and sensory characteristics of the cheeses. Increasing the salt concentrations caused a proportional decrease in proteolysis determined by both urea-PAGE of caseins and RP-HPLC of peptides. With increased salt concentration, total number of lactic acid bacteria decreased. Cheeses with 1 and 2·5% salt were suitable and acceptable in odour and flavour that may be due to the proportional level of proteolysis products. In conclusion, reducing salt concentration from 4 to 2·5 and 1% had no ineligible effect on the quality and acceptability of the cheese. © 2015 Proprietors of Journal of Dairy Research.

The thermal inactivation behaviour of calf rennet and Rhizomucor miehei protease and the effects of different concentrations of these enzymes on chemical composition, proteolysis, microstructure, hardness and meltability of Malatya cheese were studied. Thermal stability and inactivation behaviour of the two enzymes were different and influenced the level of proteolysis during ripening and the meltability of the cheeses. This influence depended not only on the type of coagulant, but also on its concentration. Salt and protein contents and pH of the cheeses were influenced by the type and concentration of the coagulant enzyme. Only small effects on the microstructure of the cheeses were visualised, also related to the type and concentration of coagulant enzyme. Hardness of the cheeses was influenced by the type and concentration of the coagulant as well. In conclusion, the microbial coagulant provided a higher level of thermal stability, proteolysis and meltability values than calf rennet.

The effects of ripening temperature and adjunct cultures (Lactobacillus helveticus and Lactobacillus casei) on the volatile compounds and sensory and textural properties of white-brined cheese were investigated. Three batches of cheese were produced: cheese A was inoculated with only cheese starter culture (Lactococcus lactis subsp. lactis plus Lactococcus lactis subsp. cremoris), cheese B was inoculated with cheese starter culture plus Lactobacillus helveticus and cheese C was inoculated with cheese starter culture plus Lactobacillus casei. Cheeses were ripened at 6 or 12 °C and analyzed at 30-day intervals up to 120 days of ripening. The use of adjunct culture and ripening temperature significantly influenced the pH and proteolysis of cheeses (P < 0.05). Acids, ketones and alcohols were found at high levels in all three cheeses. Volatiles were significantly influenced by the use of the adjunct cultures, ageing and to some extent ripening temperature (P < 0.05). Textural parameters of the cheeses were significantly affected by the adjunct culture during ripening (P < 0.05). The sensory scores of the cheese samples decreased during the ripening period. An age-related bitterness was detected by the panellists in 90 or 120-day-old cheeses with added adjunct cultures. In conclusion, the use of adjunct culture and ripening at 12 °C enhanced the volatile composition and changed the texture profiles of the cheeses.

Four different types of mould-ripened Civil cheese were manufactured. A defined (nontoxigenic) strain of a Penicillium roqueforti (SC 509) was used as the secondary starter with and without addition of the whey cheese (Lor); in parallel, secondary starter-free counterparts were manufactured. Chemical composition, microbiology and proteolysis were studied during the ripening. The incorporation of whey cheese in the manufacture of mould-ripened Civil cheese altered the gross composition and adversely affected proteolysis in the cheeses. The inoculated P. roqueforti moulds appeared to grow slowly on those cheeses, and little proteolysis was evident in all cheese treatments during the first 90 days of ripening. However, sharp increases in the soluble nitrogen fractions were observed in all cheeses after 90 days. Microbiological analysis showed that the microbial counts in the cheeses were at high levels at the beginning of ripening, while their counts decreased approximately 1–2 log cfu/g towards the end of ripening.

Summary Kashar cheeses were manufactured from pure ovine (OV), bovine (BV) and caprine (CP) milk, and the chemical composition, cheese yield, proteolysis, hardness, meltability and volatile composition were studied during 90 days. Gross chemical composition, cheese yield and level of proteolysis were higher in OV cheeses than those of BV or CP cheeses. Glu, Val, Leu, Phe and Lys were the most abundant free amino acids (FAA) in the samples, and the concentrations of individual FAA were at the highest levels in OV cheeses with following BV and CP cheeses. Urea-PAGE patterns and RP-HPLC peptide profiles of the BV cheeses were completely different from the small ruminants’ milk cheeses (OV or CP). Higher and lower hardness and meltability values were observed in CP cheeses, respectively. OV cheeses resulted in higher levels of the major volatile compounds. In conclusion, the Kashar cheese made using OV milk can be recommended due to high meltability, proteolysis and volatiles.

The objective of this study was to determine the gross composition, proteolysis, and volatile and texture profiles during ripening of industrial (IND) and traditional (TRD) Beaten (Bieno sirenje) cheeses made by using ewe milk. In the course of the analyses, statistical differences were determined in some physicochemical parameters, nitrogen fractions, and total free amino acid levels between TRD and IND types of cheese. Higher levels of proteolysis were observed in IND cheeses than in TRD cheeses during ripening. Levels of residual β- and αs-caseins were 72.2 and 48.7%, respectively, in 180-d-old TRD cheeses. However, the residual levels were 52.8% for β-casein and 18% for αs-casein in IND cheeses. Similar differences were noted for the reversed-phase HPLC peptide profiles of 2 types of cheeses. Also, higher concentrations of peptides were eluted in IND cheeses than in TRD cheeses during ripening. A total of 73 volatile compounds, including alcohols (16), esters (17), acids (14), terpenes (7), ketones (5), aldehydes (4), and miscellaneous (10) were identified. The IND cheeses contained higher levels of carboxylic acids, esters, alcohols, and terpenes than the TRD cheeses; however, the same levels of methyl ketones were determined in the 2 types of cheeses at the end of ripening. These may be due to some differences (e.g., pasteurization and scalding temperature, among other factors) in the manufacture of the 2 types of Beaten cheeses. The textural profile of Beaten cheeses showed that TRD production method resulted in firmer, less fracturable, and stiffer cheeses than the IND production method. In conclusion, the results suggest that the use of industrial production method (pasteurization of cheese milk and curd scalding at 70°C) in the manufacture of Beaten ewe milk cheese enriched the volatile profile of the cheese.

The objective of the study was to determine the effects of exopolysaccharide (EPS)-producing or non-EPS-producing starters on proteolysis, physical and microstructural characteristics of full-fat or low-fat Tulum cheeses during ripening. For this purpose, Tulum cheese was manufactured using full- or low-fat milk with EPS-producing and non-EPS-producing starter cultures. Chemical composition, proteolysis, texture profiles and microstructure of the cheeses were studied during 90 days of ripening. Urea-PAGE of water-insoluble and RP-HPLC peptide profiles of water-soluble fractions of the cheeses showed that the use of starters resulted in different degradation patterns in all cheeses during ripening. Although ß-casein exhibited similar degradation patterns in all cheeses, small differences are present in ?s1-casein degradation during ripening. Reducing fat in Tulum cheese changed the RP-HPLC peptide profile of the cheeses. The use of EPS-producing cultures improved the textural characteristics and changed the microstructure and proteolysis of low-fat Tulum cheese. © 2013 Institute of Food Science and Technology.

Free fatty acids profiles of 11 different cheese varieties sold in Turkey were determined to assess the development of lipolysis. Results obtained showed that the concentrations of short chain fatty acids (C4 and C6 free fatty acids) were close in all cheeses (P > 0.05), except for Canak cheese. However, significant differences were noted among the samples for other free fatty acids including C8 to C18:2 (P < 0.05). Palmitic (C16) and oleic (C18:1) acids were the most abundant free fatty acids in all cheese samples. Principal component analysis was applied to simplify interpretation of the data and distinguish the variety of the cheeses on the plot. Canak cheese gave a dramatically different free fatty acid profile from the other cheeses, probably because of the fact that ripening of this variety is achieved in the earthenware pots for about 1 year. Van Otlu (ripened with special herbs) and Civil (ripened by spontaneously molding on its surface) cheeses differed from the others by ANOVA and principal comp...

a b s t r a c t The objective of the study was to determine the gross composition, proteolysis and volatile compounds in Gokceada goat cheese and to provide initial information on its manufac- turing and ripening processes. Gokceada goat cheese is traditionally manufactured from raw goat milk in Gokceada (Imbros) island, Canakkale, Turkey. In the present study, 23 commercial samples were characterized. Urea-polyacrylamide gel electrophoresis of the water-insoluble fractions showed that both s- and -caseins were extensively decom- posed, but -casein was less degraded compared to s-casein. RP-HPLC of peptide profiles in the water-soluble fractions demonstrated qualitative and quantitative differences among the samples. Sixty volatile compounds were identified by SPME/GC-MS technique with alcohols and esters as the principal class of volatile components in the cheeses. In general, relatively large variability in gross composition and concentration of volatile aroma was found, which probably reflects lack of standardization in the production of the cheese.

Milk from two different breeds (Gokceada and Turkish Saanen) and three different starter culture systems (starter-free, mesophilic and thermophilic cultures) were used in the manufacture of Gokceada goat cheeses. Milk from the two breeds differed in dry matter, protein, fat and ash contents. These differences were reflected on the yield and chemical composition of the cheese. The pH values and soluble nitrogen fractions (in water, 12% trichloroacetic acid, and 5% phosphotungstic acid) were significantly influenced by the starter culture systems, but not the types of milk. The degradation of αs-caseins and its degradation products sharply increased after 60 days of ripening, especially in the cheese made using mesophilic starters. Greater changes were observed in RP-HPLC peptide profiles of the cheeses made using mesophilic starter cultures during ripening; however, the breed has minor effect on peptide profile. In conclusions, cheeses made using mesophilic starter culture exhibited different proteolysis patterns during ripening and the milk from Gokceada breed increased the gross composition parameters and cheese yield.

The effect of using various combinations of Rhizomucor miehei protease and camel chymosin (100:0, 75:25, 50:50, 25:75, and 0:100, respectively) on volatile composition and sensory scores in Iranian ultrafiltered white cheese was studied during 90d of ripening. A solid-phase microextraction–gas chromatography-mass spectrometric method was used for determining the volatile compounds of the cheeses. Forty compounds including esters (12), acids (6), ketones (9), alcohols (3), and miscellaneous compounds (10) were identified. The main classes of volatile components in the cheeses are esters, miscellaneous compounds, and ketones. The type and concentration of the coagulants influenced both volatile composition and sensory scores of the cheeses. Principal component analysis separated the cheeses based on the use of 2 coagulants in various combinations and ripening time. The cheeses produced using higher concentrations of R. miehei were separately located on the plot compared with the cheeses produced using higher concentrations of camel chymosin. Sensory evaluation of the cheeses showed that, in general, the cheeses produced using higher concentrations of camel chymosin received higher body and texture and odor and flavor scores than the cheese produced using higher concentrations of R. miehei . In conclusion, 2 combinations of R. miehei and camel chymosin (75:25 and 25:75, respectively) can be successfully used for the production of Iranian ultrafiltered white cheese, considering the results of volatile composition and sensory analysis.

Due to practical and hygienic uses in storage and marketing, cheese maker preference for the use of vacuum packaging instead of the brining method has increased significantly in recent years. The objective of the paper was to study and compare the chemistry, biochemistry, and microstructure of Mihalic cheeses ripened in vacuum packaging or under brine over 360 days. Proteolysis in Mihalic cheese was quite low (e.g., pH 4.6 soluble nitrogen was lower than 15%, as a percentage of the total nitrogen, and αs1-casein was slower in comparison to other brined cheeses) due to its high salt-in-moisture and low moisture contents. Urea-PAGE patterns of the pH 4.6 insoluble fractions of the cheeses showed different degradation patterns of αs1-casein in cheeses of different ages. However, β-casein was relatively resistant to hydrolysis. Packaging the cheese in polyethylene bags significantly increased the levels of pH 4.6 soluble nitrogen, 12% trichloroacetic acid-soluble nitrogen, and free amino acids compared to the cheeses ripened in brine. The total concentrations of free amino acids (FAA) in cheeses increased after 120 days of ripening, and Glu, Val, Leu, Lys, and Phe were the most abundant FAA in the cheeses. Higher levels of these AAs were found in the cheeses ripened in polyethylene bags. The peptide profiles by reversed-phase high-performance liquid chromatography of 70% (v/v) ethanol-soluble and ethanol-insoluble fractions of the pH 4.6 soluble fraction of the cheeses revealed that slight differences were found in the levels of some peptides between cheeses. Majority of the volatile compounds were present at higher concentrations in vacuum-packaged cheeses than in those ripened in brines. The images obtained by scanning electron microscopy of the cheeses ripened in polyethylene bags were clearly different (the protein matrix was more compact) from those of brine-ripened cheeses. The cheese ripened in vacuum packaging was completely different in terms of chemical composition, proteolysis, volatile composition, and microstructure. It was concluded that the vacuum packaging may be useful to maintain the chemical and biochemical qualities of Mihalic cheese, and it is also an easy practice during ripening.

The effect of pasteurization of cheese milk and cooking temperature of the curd on proteolysis of Malatya cheese was examined during ripening. The cheeses that were characterized by the scalding of curd in hot whey were manufactured from raw or pasteurized milk and the curds were scalded in hot whey at 60, 70, 80 or 90 °C. Differences in the levels of pH 4.6-soluble nitrogen between cheeses were significant (P < 0.05) after 30 days of ripening. Urea-polyacryl-amide gel electrophoresis of the pH 4.6-insoluble fractions showed that both αs1- and β-caseins were not extensively degraded, especially after 30 days of ripening, but β-casein was less degraded than αs1-casein. Analysis of peptide profiles of the pH 4.6-soluble fractions by RP-HPLC showed significant differences in the concentrations of some peptides between the cheeses. Principal component analysis distinguished the cheeses made from raw or pasteurized milk based on their peptide profiles. The results suggest that the pasteurization of cheese milk had a greater effect on peptide profiles of cheese than scalding temperature of the curd.

Summary Twelve samples of raw milk mature Kashar cheese at different stages of ripening were collected from retail outlets. The average pH, moisture, fat-in-dry matter, protein, salt-in-dry matter and titratable acidity contents of the samples were 5.33, 39.39%, 45.20%, 27.33%, 6.62% and 0.65% (as lactic acid), respectively. Indices of proteolysis varied from 10.72% to 23.75% and 7.09% to 12.26% for pH 4.6-soluble and 12% trichloroacetic acid-soluble nitrogen fractions, respectively, and total free amino acid concentrations ranged from 6.36 to 36.03 mg Leu g−1 of cheese. The cheeses were analysed for volatile compounds by Solid Phase Microextraction and Gas Chromatography-Mass Spectrometry (GC-MS). A total of 113 compounds were detected and identified belonging to the following chemical groups: acids (eleven), esters (sixteen), ketones (sixteen), aldehydes (six), alcohols (twenty-seven), sulphur compounds (seven), terpenes (seven) and miscellaneous compounds (twenty-three). The potential effect of each compound on the flavour profile of Kashar cheese is discussed. Acids, esters, ketones and alcohols were found at considerable levels in the samples. Kashar cheeses obtained from different retail outlets displayed some differences in terms of chemical composition, proteolysis and patterns of aroma compounds; and may be attributed to their production technologies and age-related variations.

Summary The performance and relative contribution of four single strains of lactococci (UC317, NCDO763, HP or SK11) as starters for cheese-making and ripening properties of Beyaz cheeses were compared. Gross composition, secondary proteolysis (water-soluble, 12% trichloroacetic acid-soluble, 5% phosphotungstic acid-soluble nitrogen fractions and total free amino acid contents), lipolysis (acid degree values, ADV) and cheese grading were monitored during 90 days of ripening. Chemical composition of whey samples obtained during cheese-making were found to be quite similar; however, whey samples obtained from NCDO763 or HP cheeses had the highest and the lowest pH values, respectively (P 0.05). The highest concentrations of soluble nitrogen and total free amino acid contents were observed in the cheeses made with NCDO763. The cheeses made with different strains of lactococci showed marked differences in cheese proteolysis during ripening.

The objective of the present study was to investigate the influence of container material (plastic or goat-skin bag) on the growth of lactic acid bacteria in Tulum cheese during 9 months of ripening. The lactic acid bacteria in Tulum cheeses were periodically counted on MRS and M17 agars throughout ripening. Results showed that the highest counts of lactic acid bacteria on MRS or M17 were observed at the beginning of ripening and their counts decreased during later stages of ripening. The cheese samples ripened in plastic bags exhibited higher numbers of LAB on MRS and M-17 agars than those ripened in goat-skin bags. A total of 112 strains of lactic acid bacteria were isolated from Tulum cheeses ripened in plastic or goat-skin bags during ripening. The lactic acid bacteria present in the cheese were classified by Microbial Identification System (MIS) based on a comparison of the fatty acid methyl ester profiles. Different species including Enteroccocus, Lactobacillus, Streptococcus, Lactococcus and Pediococcus genera were found in unripened cheese. As ripening proceeded, the species Streptococcus and Lactococcus disappeared and the percentages of the species Enterococcus was unchanged in both containers. There were slight differences between the cheeses ripened in plastic or goat-skin bags in terms of the profiles of lactic acid bacteria isolated. Some species including L. brevis, L. mesenteroides subsp. dextranicum, P. damnosus and E. mundtii were isolated only in the cheeses ripened in plastic bags; however, L. coryniformis and L. malafermentans were isolated only in the cheeses ripened in goat-skin bags at 6 or 9 months of ripening. Also the numbers of E. faecalis isolates were higher in the cheeses ripened in plastic containers than cheeses ripened goat-skin bags at the 6 or 9 months of ripening. The results showed that Lactobacillus and Enterococcus were the predominant species in matured Tulum cheeses in both ripening containers. It seemed possible to produce Tulum cheese with similar characteristics from both the containers used.

Mould-ripened civil is a traditional cheese produced mainly in eastern Turkey. The cheese is produced with a mixture of civil and whey curd cheeses (lor). This mixture is pressed into goat skins or plastic bags and is ripened for more than three months. Naturally occurring moulds grow on the surface and inside of the cheese during ripening. In this research, 140 Penicillium roqueforti strains were isolated from 41 samples of mould-ripened civil cheese collected from Erzurum and around towns in eastern Turkey. All strains were capable of mycotoxin production and were analysed using an HPLC method. It was established that all the strains (albeit at very low levels) produced roquefortine C, penicillic acid, mycophenolic acid and patulin. The amounts of toxins were in the ranges 0.4-47.0, 0.2-43.6, 0.1-23.1 and 0.1-2.3 mg kg(-1), respectively. Patulin levels of the samples were lower than the others. The lowest level and highest total mycotoxin levels were determined as 1.2 and 70.1 mg kg(-1) respectively. The results of this preliminary study may help in the choice of secondary cultures for mould-ripened civil cheese and other mould-ripened cheeses.

PubMedID: 16162519 Turkish White-brined cheese was manufactured using Lactococcus strains (Lactococcus lactis ssp. lactis NCDO763 plus L. lactis ssp. cremoris SK11 and L. lactis ssp. lactis UC317 plus L. lactis ssp. cremoris HP) or without a starter culture, and ripened for 90 d. It was found that the use of starters significantly influenced the physical, chemical, biochemical, and sensory properties of the cheeses. Chemical composition, pH, and sensory properties of cheeses made with starter were not affected by the different starter bacteria. The levels of soluble nitrogen fractions and urea-PAGE of the pH 4.6-insoluble fractions were found to be significantly different at various stages of ripening. Urea-PAGE patterns of the pH 4.6-insoluble fractions of the cheeses showed that considerable degradation of ?s1-casein occurred and that ß-casein was more resistant to hydrolysis. The use of a starter culture significantly influenced the levels of 12% trichloroacetic acid-soluble nitrogen, 5% phosphotungstic acid-soluble nitrogen, free amino acids, total free fatty acids, and the peptide profiles (reverse phase-HPLC) of 70% (vol/vol) ethanol-soluble and insoluble fractions of the pH 4.6-soluble fraction of the cheeses. The levels of peptides in the cheeses increased during the ripening period. Principal component and hierarchical cluster analyses of electrophoretic and chromatographic results indicated that the cheeses were significantly different in terms of their peptide profiles and they were grouped based on the use and type of starter and stage of ripening. Levels of free amino acid in the cheeses differed; Leu, Glu, Phe, Lys, and Val were the most abundant amino acids. Nitrogen fractions, total free amino acids, total free fatty acids, and the levels of peptides resolved by reverse phase-HPLC increased during ripening. No significant differences were found between the sensory properties of cheeses made using a starter, but the cheese made without starter received lower scores than the cheeses made using a starter. It was found that the cheese made with strains NCDO763 plus SK11 had the best quality during ripening. It was concluded that the use of different starter bacteria caused significant differences in the quality of the cheese, and that each starter culture contributed to proteolysis to a different degree. © American Dairy Science Association, 2005. This research was funded in part by Scientific Research Project Units of Cukurova University (Adana, Turkey) .

Turkish White cheese is a brined (or a pickled) cheese variety with a soft or semi-hard texture and a salty, acid taste. Some aspects of this cheese are reviewed: e.g., milk supply, use of starters and enzymes, manufacturing technology, chemical composition and microflora, chemical and biochemical changes during ripening in brine. Several characteristics of Turkish White cheese are compared to other White brined cheese varieties such as Feta and Domiati. The findings of this review suggest that future research on Turkish White cheese should characterise the changes in microflora, biochemistry and texture during ripening. Previous studies tended to focus on the chemical composition of Turkish White cheese, and little attention was directed towards the detailed characterisation of nitrogen fractions, flavour compounds, rheological and microbiological properties and their effects on the quality of the end-product.

The effects of use of adjunct cultures (Lactobacillus helveticusandLb. casei) and ripening temperatures (6 or 12 °C) on proteolysis and angiotensin-I converting enzyme (ACE)-inhibitory activity in white-brined cheeses were investigated during 120 d ripening. Proteolysis was monitored by urea-polyacrylamide gel electrophoresis (urea-PAGE) and reversed phase-HPLC (RP-HPLC) of water-insoluble and -soluble fractions of the cheeses, respectively. Urea-PAGE patterns of the samples revealed that the intensities of the bands representing casein fractions decreased in the experimental cheeses, being more pronounced in the cheeses made with adjunct cultures. Similarly, peptide profiles and the concentrations of individual and total free amino acids were influenced by both the adjunct cultures and ripening temperatures. The ACE-inhibitory activity of the water-soluble extracts of the cheeses were higher in the cheeses made using adjunct cultures (especiallyLb. helveticus) and ripened at 12 °C. The ACE-inhibitory activity did not decrease during ripening. The contribution ofLb. helveticusto the development of proteolysis and ACE-inhibitory peptide activities were higher than that ofLb. casei. To conclude, the use ofLb. helveticusas adjunct culture in white-brined cheese and ripening at 12 °C would be recommended to obtain white-brined cheese with high ACE-I-inhibitory peptides activity and higher levels of preoteolysis.

In this study, four different types of mould-ripened Civil cheese were manufactured. A defined (nontoxigenic) strain of a Penicillium roqueforti (SC 509) was used as secondary starter for the manufacture of mould-ripened Civil cheese with and without addition of the whey cheese Lor; in parallel, secondary starter-free counterparts were manufactured. A total of 83 compounds were identified. Ketones, alcohols and esters were the principal classes of volatile components. Principal component analysis of the headspace volatiles grouped cheeses by age and type. P. roqueforti inoculated cheese was clearly separated from the other cheeses at 180 days of ripening, and these cheeses were characterised with high levels of ketones (e.g., 2-butanone, 2-heptanone). Differences in the panel scores between the cheese samples were not significant during the first stage of ripening (up to 60 days); as ripening proceeded, these differences were become evident and P. roqueforti inoculated cheeses received higher scores than others. Addition of Lor in the manufacture of mould-ripened Civil cheese caused lower points by the sensory panel, and the cheese inoculated with P. roqueforti and Lor-free was the best type of mould-ripened Civil cheese. The results showed that the use of P. roqueforti in the manufacture of mould-ripened Civil cheese has significant impact on the volatile profiles and sensory attributes.

Moldy Civil is a mold-ripened variety of cheese produced mainly in eastern Turkey. This cheese is produced with Civil cheese and whey curd cheese (Lor). Civil cheese has had a geographical presence since 2009 and is manufactured with skim milk. In the production of Moldy Civil cheese, Civil cheese or a mixture of Civil and Lor cheese is pressed into goat skins or plastic bags and ripened for 3 months or longer. During the ripening period, natural contaminating molds grow on the surface of and inside the cheese. In this study, 186 mold strains were isolated from 41 samples of Moldy Civil cheese, and 165 of these strains were identified as Penicillium roqueforti. Identification and mycotoxicologic analyses were conducted using morphotypic and molecular methods. PCR amplicons of the ITS1-5.8S-ITS4 region were subjected to sequence analysis. This research is the first using molecular methods on Moldy Civil cheese. Mycotoxicologic analyses were conducted using thin-layer chromatography, and random amplified polymorphic DNA genotypes were determined using the ari1 primer. Of 165 isolates, only 28 produced no penicillic acid, P. roqueforti toxin, or roquefortine.

In this study, the influence of rennet concentration on chemical composition, proteolysis and sensory properties of Halloumi cheese was evaluated during ripening. Halloumi cheese was manufactured from raw cow's milk using four levels of rennet (0.2, 0.25, 0.3 or 0.4 g rennet/kg cheese milk) and was ripened for 60 days at 4C. Proteolysis in the cheese was measured by determining soluble nitrogen fractions both in water and in 12% trichloroacetic acid as well as by patterns of urea–polyacrylamide gel electrophoresis. Cheese grading was performed by an expert panel throughout the ripening period. The chemical composition of the cheese was significantly influenced by the level of rennet, which could be related to the differences in moisture contents of the cheese. The levels of water- or 12% trichloroacetic acid-soluble nitrogen fractions and degradations of αs1- or β-caseins were quite low during the cheese maturation. No relation was found between rennet concentrations and casein degradation in the cheese during ripening. Results indicated that the different concentrations of rennet did not significantly influence the proteolytic profiles and sensory scores of Halloumi cheese; that is, an amount of rennet between 0.2 and 0.4 g rennet/kg cheese milk resulted in a similar quality of Halloumi cheese. PRACTICAL APPLICATIONS Halloumi cheese was manufactured from raw cow's milk using different concentrations of rennet and was ripened for 60 days. The chemical composition of the cheese was influenced by different concentrations of rennet; however, ripening indices (proteolysis) and sensory attributes were identical in all samples. It is concluded that different concentrations of rennet (0.2–0.4 g rennet/kg cheese milk) have no significant influence on the ripening characteristics of Halloumi.

Changes in chemical composition, proteolysis, lipolysis, texture, melting and sensory properties of low-fat Kashar cheese made with three different fat replacers (Simplesse® D-100, Avicel Plus® CM 2159 or β-glucan) were investigated throughout ripening. The low-fat cheeses made with fat replacers were compared with full- and low-fat counterparts as controls. Reduction of fat caused increases in moisture and protein contents and decreases in moisture-in-non fat substance and yield values in low-fat cheeses. The use of fat replacers in the manufacture of low-fat Kashar cheese increased water binding capacity and improved overall quality of the cheeses. Use of fat replacer in low-fat cheese making has enhanced cheese proteolysis. All samples underwent lipolysis during ripening and low-fat cheeses with fat replacers had higher level of total free fatty acid than full- or low-fat control cheeses. Texture attributes and meltability significantly increased with addition of fat replacers. Sensory scores showed that the full-fat cheese was awarded best in all stages of ripening and low-fat variant of Kashar cheeses have inferior quality. However, fat replacers except β-glucan improved the appearance, texture and flavour attributes of low-fat cheeses. When the fat replacers are compared, the low-fat cheese with Avicel Plus® CM 2159 was highly acceptable and had sensory attributes closest to full-fat Kashar cheese.

Tulum cheeses were manufactured from raw ewe's milk and ripened in goat's skin bags (tulums) or plastic containers to understand the effect of ripening container on the chemical composition, biochemistry, microbiology, and volatile composition of Tulum cheeses during 150 d of ripening. Chemical compositions of the cheeses ripened in tulums were significantly different and the moisture contents decreased rapidly in those cheeses because of the porous structure of the tulum. Higher microbial counts were detected in the cheeses ripened in plastic than in cheeses ripened in tulums. Differences in nitrogenous compounds and total free AA of the cheeses were not significant. Total concentrations of free AA in cheeses increased with age and Glu, Ala, Val, Leu, and Phe were the most abundant AA in the cheeses. Urea-PAGE of pH 4.6-insoluble fractions of the cheeses during ripening showed similar degradation patterns in all cheeses. Peptide profiles by reversed-phase HPLC of pH 4.6- and ethanol-soluble or ethanol-insoluble fractions of the cheeses revealed only minor differences in the concentrations of some peptides among the cheeses; however, age-related changes in peptide concentrations were significantly different among the cheeses. Cheeses were analyzed at 90 d of ripening for volatile compounds by solid-phase microextraction gas chromatography-mass spectrometry. One hundred volatile components were identified, including 11 acids, 16 esters, 12 methyl ketones, 7 aldehydes, 22 alcohols, 7 sulfur compounds, 6 terpenes, and 19 miscellaneous compounds. The main components were short-chain fatty acids, 2-butanone, diacetyl, and primary alcohols. Quantitative differences in several volatile compounds were evident among the cheeses. Cheeses ripened in tulums or plastic had similar aroma patterns, but the concentrations of some components were different.

Du Beyaz peynir (fromage turc saumure) a ete conserve durant neuf semaines dans des saumures de quatre concentrations en sel differentes (12, 14, 16 ou 18 g NaCl.100 g -1 ) a 7 ± 1 °C, dans le but d'etudier l'effet de la concentration en sel durant la maturation sur la composition, la proteolyse, la texture et les caracteristiques sensorielles du fromage. Des differences significatives ont ete constatees pour les valeurs de pH, de la matiere seche, de la teneur en proteine totale, la durete, la fraction d'azote soluble dans l'acide trichloroacetique a 12 % (w/v), et l'hydrolyse de la caseine β, tandis qu'aucune difference n'a ete observee pour la quantite d'azote soluble dans l'eau et l'hydrolyse de la caseine α s1 . Les fromages matures-dans la saumure a 12 % de sel ont obtenu les meilleurs scores pour la flaveur et l'acceptabilite globale. Les resultats obtenus dans cette etude montrent que la proteolyse et l'acceptabilite globale des fromages Beyaz peynir sont moins elevees pour les fromages matures dans des saumures de concentrations en sel superieures a 14 %.

Bu çalışmada, bazı Lactococcus spp. cinsi starter bakteriler ile Lactobacillus spp. cinsi yardımcı kültür bakteri kombinasyonları (A peyniri: Lactococcus lactis ssp. lactis ve Lactococcus lactis ssp. cremoris karışımı klasik kültür, B peyniri: klasik kültür + Lactobacillus helveticus ve C peyniri: klasik kültür + Lactobacillus casei) kullanılarak Beyaz peynir üretilmiş ve iki farklı sıcaklıkta (6 °C ve 12 °C) 120 gün süre ile olgunlaştırılmıştır. Yardımcı kültür kullanımı peynirin fiziksel, kimyasal, mikrobiyolojik, tekstürel, duyusal özellikleri ile aroma oluşumu üzerine ve Angiotensin dönüştürücü enzim-inhibisyon aktivitesi üzerine önemli düzeyde etkili olduğu saptanmıştır. Yardımcı kültür ilave edilen peynirlerin pH ve titrasyon asitliği değerlerinin birbirinden farklı değerler aldığı belirlenmiştir. Yardımcı kültür kullanımı, duyusal ve tekstürel özellikler üzerinde farklılık yaratırken, farklı sıcaklıkta olgunlaştırılan peynirler arasında bu parametreler açısından bir farklılık bulunamamıştır. Yardımcı kültür kullanımının suda çözünen ve % 12 TCA’da çözünen azot değerlerinde farklılık gösterirken, farklı depolama sıcaklıklarının etkisi sınırlı olmuştur. Peynirlerin serbest amino asit düzeyleri arasında farklılık bulunmuş ve en yüksek konsantrasyonda Leu, Glu, Arg ve Lys amino asitlerinin olduğu belirlenmiştir. Peynirlerin suda çözünmeyen fraksiyonlarının üre-jel elektroforezinde, ?s1-kazeinin önemli düzeyde parçalandığı, ?-kazeinin hidrolize karşı daha dirençli olduğu belirlenmiştir. 120 günlük olgunlaşma süresince RP-HPLC peptit profilleri üzerine yardımcı kültür kullanımının ve farklı olgunlaşma sıcaklıklarının önemli düzeyde etkilerinin olduğu bulunmuştur. Peynirler arasında aroma profili açısından da farklılıklar belirlenmiş olup, farklı sıcaklıkta olgunlaştırmanın aroma üzerine etkisinin de önemli olduğu saptanmıştır. Olgunlaşma süresi ilerledikçe her iki depolama sıcaklığında peynirlerinin ACE-inhibisyon aktivitesinde artış olduğu saptanmıştır. Olgunlaşma süresince peynirlerin azot fraksiyonları, toplam FAA, RP-HPLC ile belirlenen peptit konsantrasyonu, aroma profilinde artışlar olmuş, tekstürel özellikleri olgunlaşma süresi, sıcaklık ve yardımcı kültür farklılığı parametrelerinin sertlik değerleri üzerine etkili olduğu, diğer parametreler üzerine ise olgunlaşma sıcaklığının etkisinin önemli olmadığı bulunmuştur. Yardımcı kültür kullanımının ve farklı depolama sıcaklıklarının, Beyaz peynir kalitesinde önemli ölçüde etkili olduğu sonucuna varılmıştır. In this study, Turkish white-brined cheese was manufactured using Lactococcus spp. as starter culture and Lactobacillus spp. strains as adjunt culture (A cheese: Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris traditional cultures, B cheese: traditional cultures + Lactobacillus helveticus and C cheese: traditional cultures + Lactobacillus casei) and experimental cheese were different temperature (6 °C ve 12 °C) ripened for 120 days. It was found that the use of adjunt culture have significantly influenced the physical, chemical, microbiological, textural and sensory properties, ACE-inhibition activity and flavor formation. pH and titratable acidity values were significantly different in adjunt culture added cheeses. Use of adjunt culture resulted in differences in sensory and textural properties of the cheeses; however, no significant differences were found between cheeses in terms of ripening temperature. In addiation, water and 12 % TCA- soluble fractions were found to be significantly different but, the effect of ripening temperature was limited on the parameters. There were significant differences among the concentrations of free amino acids levels in cheeses and Leu, Phe, Glu, Arg and Lys were the most abundant amino acids. Urea-gel electrophoresis of waterinsoluble fractions of the cheeses showed that ?s1-casein significantly degraded during ripening; however, ?-casein was more resistant to to hydrolysis. RP-HPLC peptide profiles were significantly influenced by adjunct culture and ripening temperature. Differences between cheeses have been determined in terms of aroma profile, and the effect of ripening temperatures on aroma profile of the cheese samples were statistically significant. As ripening period progressed, ACE-inhibitory activities of the cheeses increased in both storage temperature. Nitrogen fractions, total FAA, RP-HPLC peptide concentrations increased during ripening, and ripening period, temperature and adjunct culture has significantly influenced the textural properties (especially hardness values); however, other paramters were not influenced by ripening temperature. İt was concluded that use of different adjunt culture and ripening temperature significantly affected the quality of white-brined cheese.