1. A switch from noncanonical to canonical Wnt signaling stops neuroblast migration through a Slt-Robo and RGA-9b/ARHGAP-dependent mechanism
- Author
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Rella, Lorenzo, Fernandes Póvoa, Euclides E, Mars, Jonas, Ebbing, Annabel L P, Schoppink, Luc, Betist, Marco C, Korswagen, Hendrik C, Sub Developmental Biology, Developmental Biology, Sub Developmental Biology, Developmental Biology, and Hubrecht Institute for Developmental Biology and Stem Cell Research
- Subjects
Slt-Robo ,GTPase-Activating Proteins/metabolism ,Nerve Tissue Proteins ,Biology ,Caenorhabditis elegans Proteins/genetics ,Wnt ,Neuroblast ,Neural Stem Cells ,Cell Movement ,Neuroblast migration ,Receptors ,Cell Movement/genetics ,Animals ,Nerve Tissue Proteins/genetics ,Cell migration ,Receptors, Immunologic ,Caenorhabditis elegans Proteins ,Caenorhabditis elegans ,General ,Wnt Signaling Pathway ,chemistry.chemical_classification ,Multidisciplinary ,Receptors, Immunologic/genetics ,Mechanism (biology) ,Immunologic/genetics ,GTPase-Activating Proteins ,Rho GTPases ,Wnt signaling pathway ,Neural Stem Cells/cytology ,Biological Sciences ,Cell biology ,Pathway interactions ,chemistry ,Gene Expression Regulation ,C. elegans ,Signal transduction ,Glycoprotein - Abstract
Members of the Wnt family of secreted glycoproteins regulate cell migration through distinct canonical and noncanonical signaling pathways. Studies of vertebrate development and disease have shown that these pathways can have opposing effects on cell migration, but the mechanism of this functional interplay is not known. In the nematode Caenorhabditis elegans, a switch from noncanonical to canonical Wnt signaling terminates the long-range migration of the QR neuroblast descendants, providing a tractable system to study this mechanism in vivo. Here, we show that noncanonical Wnt signaling acts through PIX-1/RhoGEF, while canonical signaling directly activates the Slt-Robo pathway component EVA-1/EVA1C and the Rho GTPase-activating protein RGA-9b/ARHGAP, which are required for migration inhibition. Our results support a model in which cross-talk between noncanonical and canonical Wnt signaling occurs through antagonistic regulation of the Rho GTPases that drive cell migration.
- Published
- 2021