1. NDAT suppresses pro-inflammatory gene expression to enhance resveratrol-induced anti-proliferation in oral cancer cells.
- Author
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Ho Y, Wu CY, Chin YT, Li ZL, Pan YS, Huang TY, Su PY, Lee SY, Crawford DR, Su KW, Chiu HC, Shih YJ, Changou CA, Yang YSH, Whang-Peng J, Chen YR, Lin HY, Mousa SA, Davis PJ, and Wang K
- Subjects
- B7-H1 Antigen genetics, B7-H1 Antigen immunology, Cell Line, Tumor, Cell Proliferation drug effects, Cyclin D1 genetics, Cyclin D1 metabolism, Cyclooxygenase 2 genetics, Cyclooxygenase 2 immunology, Drug Synergism, Gene Expression, Humans, Mouth Neoplasms genetics, Mouth Neoplasms immunology, STAT3 Transcription Factor genetics, STAT3 Transcription Factor immunology, Thyroxine pharmacology, Mouth Neoplasms physiopathology, Polyglactin 910 pharmacology, Resveratrol pharmacology, Thyroxine analogs & derivatives
- Abstract
Nano-diamino-tetrac (NDAT), a tetraiodothyroxine deaminated nano-particulated analog, has shown to inhibit expression of pro-inflammatory genes. NDAT inhibits expression of programmed death-ligand 1 (PD-L1). On the other hand, in addition to inhibiting inflammatory effect, the stilbene, resveratrol induces expression of cyclooxygenase-2 (COX-2) and its accumulation. Sequentially, inducible COX-2 complexes with p53 and induces p53-dependent anti-proliferation. In current study, we investigated mechanisms involved in combined treatment of NDAT and resveratrol on anti-proliferation in human oral cancer cells. Both resveratrol and NDAT inhibited expression of pro-inflammatory IL-1β and TNF-α. They also inhibited expression of CCND1 and PD-L1. Both resveratrol and NDAT induced BAD expression but only resveratrol induced COX-2 expression in both OEC-M1 and SCC-25 cells. Combined treatment attenuated gene expression significantly compared with resveratrol treatment in both cancer cell lines. Resveratrol reduced nuclear PD-L1 accumulation which was enhanced by a STAT3 inhibitor, S31-201 or NDAT suggesting that NDAT may inactivate STAT3 to inhibit PD-L1 accumulation. In the presence of T
4 , NDAT further enhanced resveratrol-induced anti-proliferation in both cancer cell lines. These findings provide a novel understanding of the inhibition of NDAT in thyroxine-induced pro-inflammatory effect on resveratrol-induced anticancer properties., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
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