1. Early resuscitation with exendin-4 alleviates acute lung injury after hemorrhagic shock in rats.
- Author
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Song X, Chen G, Luo X, You G, Yin Y, Wang Y, Zhao L, and Zhou H
- Subjects
- Acute Lung Injury etiology, Acute Lung Injury metabolism, Acute Lung Injury pathology, Animals, Antioxidants pharmacology, Biomarkers metabolism, Combined Modality Therapy, Exenatide, Fluid Therapy, Infusions, Intravenous, Male, Oxidative Stress drug effects, Peptides pharmacology, Random Allocation, Rats, Rats, Wistar, Shock, Hemorrhagic complications, Treatment Outcome, Venoms pharmacology, Acute Lung Injury prevention & control, Antioxidants therapeutic use, Peptides therapeutic use, Resuscitation methods, Shock, Hemorrhagic therapy, Venoms therapeutic use
- Abstract
Background: Oxidative stress induced by hemorrhagic shock (HS) is known to initiate a systemic inflammatory response, which leads to subsequent acute lung injury. This study is aimed to assess the efficacy of exendin-4 (Ex-4) in attenuating lung injury in a rat model of HS and resuscitation (HS/R)., Methods: HS was induced in sodium pentobarbital-anesthetized adult male Wistar rats by withdrawing blood to maintain a mean arterial pressure of 30-35 mm Hg for 50 min. Then, the animals received Ex-4 (5 μg/kg) or vehicle (saline) intravenously and were resuscitated with a volume of normal saline 1.5 times that of the shed blood volume. Mean arterial pressure was measured throughout the experiment, and acid-base status, oxidative stress, inflammation, and lung injury were evaluated at 2 h after resuscitation., Results: Ex-4 infusion reduced the methemoglobin content, the malondialdehyde content, the myeloperoxidase activity, and the expression of tumor necrosis factor-α and interleukin-6 in the lungs. The histologic injury was also markedly decreased in the Ex-4 group compared with the vehicle group., Conclusions: Ex-4 ameliorates the oxidative stress, inflammatory response, and subsequent acute lung injury occurring after HS/R. Although future studies are required to elucidate the underlying mechanism, our results indicate that Ex-4 infusion may be a promising strategy for improving lung injury in the treatment of HS., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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