Salaun B, De Smedt J, Vernhes C, Moureau A, Öner D, Bastian AR, Janssens M, Balla-Jhagjhoorsingh S, Aerssens J, Lambert C, Coenen S, Butler CC, Drysdale SB, Wildenbeest JG, Pollard AJ, Openshaw PJM, and Bont L
Introduction: The immune mechanisms supporting partial protection from reinfection and disease by the respiratory syncytial virus (RSV) have not been fully characterized. In older adults, symptoms are typically mild but can be serious in patients with comorbidities when the infection extends to the lower respiratory tract., Methods: This study formed part of the RESCEU older-adults prospective-cohort study in Northern Europe (2017-2019; NCT03621930) in which a thousand participants were followed over an RSV season. Peripheral-blood samples (taken pre-season, post-season, during illness and convalescence) were analyzed from participants who (i) had a symptomatic acute respiratory tract infection by RSV (RSV-ARTI; N=35) or (ii) asymptomatic RSV infection (RSV-Asymptomatic; N=16). These analyses included evaluations of antibody (Fc-mediated-) functional features and cell-mediated immunity, in which univariate and machine-learning (ML) models were used to explore differences between groups., Results: Pre-RSV-season peripheral-blood biomarkers were predictive of symptomatic RSV infection. T-cell data were more predictive than functional antibody data (area under receiver operating characteristic curve [AUROC] for the models were 99% and 76%, respectively). The pre-RSV season T-cell phenotypes which were selected by the ML modelling and which were more frequent in RSV-Asymptomatic group than in the RSV-ARTI group, coincided with prominent phenotypes identified during convalescence from RSV-ARTI (e.g., IFN-γ+, TNF-α+ and CD40L+ for CD4+, and IFN-γ+ and 4-1BB+ for CD8+)., Conclusion: The evaluation and statistical modelling of numerous immunological parameters over the RSV season suggests a primary role of cellular immunity in preventing symptomatic RSV infections in older adults., Competing Interests: Authors CV and AM were employed by the company Sanofi during the development of the study and own stock options in the company. Authors SB-J, AB, JA and DÖ were employed by the company Janssen Pharmaceutica NV. Authors SB-J, AB and JA own stocks from Johnson & Johnson. Authors MJ, BS, CL, and JD were employed by the company GSK, and BS and CL own stocks from the company. Author CL had submitted a patent application for COMPOSITIONS AND METHODS FOR IMPROVING THE IMMUNE RESPONSE TO SARS-COV2. During this time, he was a GSK representative as a co-leader for a subgroup in the Adventitious Virus Detection Technologies Interest Groups AVDTIG. Author SD has been an investigator for clinical trials of vaccines and antimicrobials for pharmaceutical companies including AstraZeneca, Merck, Pfizer, Valneva, Iliad, Sanofi, and Janssen, and previously sat on RSV advisory boards for Sanofi and Merck. Author JW was a member of Janssen and Sanofi advisory boards. She also worked closely together with author LB on many projects and clinical studies for which LB received grants. Author AP received grants from: Gates, Wellcome, MRC, NIHR and European Commission IMI RESCEU. During this time the laboratory assays were provided by GSK. Author AP is a contributor to intellectual property licensed by Oxford University Innovation to AstraZeneca. He also received consulting fees from Shionogi. He was chair of DHSC’s Joint Committee on Vaccination and Immunisation and also a member of WHOs SAGE until 2022. Author PO received the RESCEU EU IMI award and consulting fees from: GSK, Moderna, Janssen, Seqirus and Pfizer. He also received payments or honoraria from Moderna, Medscape and support for attending meetings for Moderna. During this project he was also a school governor for Sidcot, Somerset. He received grants from UKRI and MRC EMINENT. Author LB has regular interaction with pharmaceutical and other industrial partners. UMCU has received funding for investigator-initiated studies from AbbVie, MedImmune, AstraZeneca, Sanofi, Janssen, Pfizer, MSD and MeMed Diagnostics. UMCU has received funding for the RSV GOLD study from the Bill and Melinda Gates Foundation. UMCU has received funding as part of the public private partnership IMI funded RESCEU and PROMISE projects with partners GSK, Novavax, Janssen, AstraZeneca, Pfizer and Sanofi. UMCU has received funding by Julius Clinical for participating in clinical studies sponsored by MedImmune and Pfizer. UMCU received funding for consultation and invited lectures by AbbVie, MedImmune, Ablynx, Bavaria Nordic, MabXience, GSK, Novavax, Pfizer, Moderna, Astrazeneca, MSD, Sanofi, Genzyme, Janssen. Author LB is the founding chairman of the ReSViNET Foundation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Salaun, De Smedt, Vernhes, Moureau, Öner, Bastian, Janssens, Balla-Jhagjhoorsingh, Aerssens, Lambert, Coenen, Butler, Drysdale, Wildenbeest, Pollard, Openshaw and Bont.)