1. Combining lung-protective strategies in experimental acute lung injury: The impact of high-frequency partial liquid ventilation.
- Author
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Rotta AT, Viana ME, Wiryawan B, Sargentelli GA, Dowhy MS, Zin WA, and Fuhrman BP
- Subjects
- Aldehydes analysis, Animals, Blood Gas Analysis, Disease Models, Animal, Lipopolysaccharides adverse effects, Lung metabolism, Lung pathology, Malondialdehyde analysis, Rabbits, Respiratory Distress Syndrome etiology, Sodium Chloride adverse effects, Chest Wall Oscillation methods, Liquid Ventilation methods, Respiratory Distress Syndrome therapy
- Abstract
Objective: To evaluate the independent and combined effects of high-frequency oscillatory ventilation (HFOV) and partial liquid ventilation (PLV) on gas exchange, pulmonary histopathology, inflammation, and oxidative tissue damage in an animal model of acute lung injury., Design: Prospective, randomized animal study., Setting: Research laboratory of a health sciences university., Subjects: Fifty New Zealand White rabbits., Interventions: Juvenile rabbits injured by lipopolysaccharide infusion and saline lung lavage were assigned to conventional ventilation (CMV), PLV, HFOV, or high-frequency partial liquid ventilation (HF-PLV) with a full or half dose (HF-PLV1/2) of perfluorochemical (PFC). Uninjured ventilated animals served as controls. Arterial blood gases were obtained every 30 mins during the 4-hr study. Histopathologic evaluation was performed using a lung injury scoring system. Oxidative lung injury was assessed by measuring malondialdehyde and 4-hydroxynonenal in lung homogenates., Measurements and Main Results: HFOV, PLV, or a combination of both methods (HF-PLV) resulted in significantly improved oxygenation, more favorable lung histopathology, reduced neutrophil infiltration, and attenuated oxidative damage compared with CMV. HF-PLV with a full PFC dose did not provide any additional benefit compared with HFOV alone. HF-PLV1/2 was associated with decreased pulmonary leukostasis compared with HF-PLV., Conclusions: The combination of HFOV and PLV (HF-PLV) does not provide any additional benefit compared with HFOV or PLV alone in a combined model of lung injury when lung recruitment and volume optimization can be achieved. The use of a lower PFC dose (HF-PLV1/2) is associated with decreased pulmonary leukostasis compared with HF-PLV and deserves further study.
- Published
- 2006
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