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Abstract This work aimed to study in the freshwater gastropod Planorbarius corneus the effects of acute (2 days) and subchronic (14 days) exposures to an environmental concentration of the organophosphate azinphos-methyl on different reproductive parameters, offspring survival and B-esterase activities in gonads and in the whole organism soft tissue. The acute exposure inhibited only carboxylesterase activity in both tissues while the subchronic exposure also inhibited cholinesterase activity, decreased the number of hatched-eggs and increased offspring lethality (92%). On the other hand, B-esterases in gonads were more effective biomarkers than B-esterases in the whole organism due their inhibition appeared earlier in time (cholinesterase activity) and their activity remained inhibited for a longer time (carboxylesterase activity) when recovery studies were performed. We concluded that B-esterases and reproductive parameters can be used as effect biomarkers of aquatic contamination with azinphos-methyl. Our studies showed that a 14 days exposure to an environmental concentration of azinphos-methyl produced severe signs of toxicity in adult organisms, egg masses and juveniles that could cause negative effects at the population level in contaminated environments. Graphical abstract Unlabelled Image Highlights • The acute exposure of Planorbarius corneus to azinphos-methyl inhibited carboxylesterses that cannot recover in gonads. • The subchronic exposure inhibited cholinesterases and carboxylesterases. • A decrease of the number of hatched eggs was observed when the masses were exposed. • The survival of the juveniles decreased in a 92 % after one month of hatching. • B-esterases measured in the gonads result better biomarkers than in the whole organism soft tissue. [ABSTRACT FROM AUTHOR]

Abstract: Chlorpyrifos is an organophosphate insecticide used for pest control on a number of food crops in many parts of the world. In recent years, there has been an important decrease in the number of organisms of Planorbarius corneus. Since the presence of pesticides in the water can be one of the reasons for this decrease, it is very important to study the effect of subchronic exposure to environmental concentrations of pesticides on these organisms. The aim of the present work was to investigate different effects of the subchronic exposure to low concentrations of the organophosphate chlorpyrifos in P. corneus and the possibility to use these as biomarkers. To this end, we have exposed the organisms to 0.4 and 5μgL−1 of chlorpyrifos for 14 days and recorded the number of egg masses, the number of eggs per mass, the number of eggs without embryo, the time for hatching, and the % of hatching and survival. We have also determined the activities of cholinesterases, carboxylesterases and glutathione S-transferase in whole organism soft tissue and in the gonads. A 14 days exposure to 0.4μgL−1 caused an increase in the number of egg masses without eggs and a decrease in carboxylesterases measured with p-nitrophenyl butyrate. However the exposure to 5μgL−1 also caused an increase in the time for hatching, a decrease in the % of hatching and survival and also inhibition of cholinesterases and carboxylesterases with p-nitrophenyl acetate and butyrate. In contrast, the glutathione S-transferase has not been modified with the tested concentrations. We concluded that when P. corneus exposed to chlorpyrifos for 14 days, the CES determined with p-nitrophenyl butyrate proved to be the most sensitive biomarker. However, exposure to environmental concentrations showed a decrease in the reproduction ability which could cause a decrease in the number of organisms of this species. [Copyright &y& Elsevier]