1. Repair of experimentally induced large osteochondral defects in rabbit knee with various concentrations of Escherichia coli-derived recombinant human bone morphogenetic protein-2.
- Author
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Tokuhara Y, Wakitani S, Imai Y, Kawaguchi A, Fukunaga K, Kim M, Kadoya Y, and Takaoka K
- Subjects
- Animals, Bone Morphogenetic Protein 2, Cartilage, Articular injuries, Cartilage, Articular pathology, Disease Models, Animal, Dose-Response Relationship, Drug, Female, Humans, Patella injuries, Patella pathology, Rabbits, Bone Morphogenetic Proteins pharmacology, Cartilage, Articular drug effects, Escherichia coli metabolism, Patella drug effects, Recombinant Proteins pharmacology, Stifle, Transforming Growth Factor beta pharmacology, Wound Healing drug effects
- Abstract
Effective therapies for the regeneration of large osteochondral defects are still lacking; however, various approaches have been used. We evaluated the efficacy of Escherichia coli-derived dimeric recombinant human BMP-2 (E-rhBMP-2) for the repair of large osteochondral defects in a rabbit model. Osteochondral defects made in the femoral patellar groove of the knee were treated by transplanting gelatin sponges onto which no or various doses of E-rhBMP-2 were loaded. The outcomes were compared with those of an untreated control group four, 12 and 24 weeks after transplantation. At early time points, the cartilage tissue was repaired in a dose-dependent manner, and bone repair was accelerated in the defects treated with high doses of E-rhBMP-2. At 24 weeks, the repair of cartilage tissue was better with E-rhBMP-2 treatment, even at low doses, than without E-rhBMP-2 treatment. Our findings suggest that the use of E-rhBMP-2 improves and accelerates the repair of osteochondral defects in a rabbit model.
- Published
- 2010
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