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Your search keyword '"TCR transgenic mouse"' showing total 2 results
Start Over Descriptor "TCR transgenic mouse" Topic receptors, antigen, t-cell, alpha-beta
2 results on '"TCR transgenic mouse"'

1. Inflammatory and anti-inflammatory states of adipose tissue in transgenic mice bearing a single TCR.

Author

Matsumoto A, Taniguchi K, Takeda N, Yamamura KI, Arai S, and Miyazaki T

Subjects
Adipose Tissue pathology, Animals, Dietary Fats adverse effects, Dietary Fats pharmacology, Inflammation chemically induced, Inflammation genetics, Inflammation immunology, Inflammation pathology, Macrophages pathology, Mice, Mice, Transgenic, Obesity chemically induced, Obesity genetics, Obesity pathology, Receptors, Antigen, T-Cell, alpha-beta genetics, T-Lymphocytes, Regulatory pathology, Adipose Tissue immunology, Macrophages immunology, Obesity immunology, Receptors, Antigen, T-Cell, alpha-beta immunology, T-Lymphocytes, Regulatory immunology
Abstract

Obesity is accompanied by chronic, low-grade inflammation in adipose tissue, which is associated with insulin resistance and consequent multiple metabolic diseases. In addition to M1 macrophage infiltration, multiple involvements of adipose tissue T lymphocytes in the progression of inflammation have been highlighted recently. Here, we isolated a specific Vα5/Vβ8.2 TCR-bearing T cell that accumulated in obese adipose tissue of mice, and generated transgenic mice expressing this TCR. Under lean conditions with a normal chow diet, CD4+FoxP3+ Treg cells and M2 macrophages increased in adipose tissue with ageing in wild-type mice, but not in transgenic mice. However, both mice exhibited no obvious adipose tissue inflammation such as the formation of crown-like structures (CLSs) of infiltrating macrophages. When fed a high-fat diet, the proportion of adipose tissue Treg cells was markedly small at a similar level in transgenic and wild-type mice. Both types of mice exhibited comparable inflammatory states in adipose tissue, including vast formation of macrophage CLSs, accompanied by insulin resistance. Together, our findings suggest that the absence of an increase in Treg cells and M2 macrophages is not sufficient to initiate inflammatory macrophage infiltration in lean adipose tissue and also provide a new view about the involvement of T cells in promoting obesity-associated inflammation., (© The Author 2017. Published by Oxford University Press on behalf of The Japanese Society for Immunology.)

Published
2017
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2. Swift development of protective effector functions in naive CD8(+) T cells against malaria liver stages

Author

Ryo Abe, Fidel Zavala, Alexandre Morrot, Julius C. R. Hafalla, Gen Ichiro Sano, and Juan J. Lafaille

Subjects
Receptors, Antigen, T-Cell, alpha-beta, CD8-Positive T-Lymphocytes, Lymphocyte Activation, Interleukin 21, Epitopes, Mice, 0302 clinical medicine, Immunology and Allergy, Cytotoxic T cell, Interferon gamma, IL-2 receptor, 0303 health sciences, Mice, Inbred BALB C, Membrane Glycoproteins, Cell Differentiation, 3. Good health, medicine.anatomical_structure, Liver, in vivo differentiation, Original Article, Plasmodium yoelii, Cell Division, medicine.drug, Pore Forming Cytotoxic Proteins, TCR transgenic mouse, T cell, Immunology, malaria, Antigens, Protozoan, Mice, Transgenic, Biology, CD8+ T cells, 03 medical and health sciences, Interferon-gamma, Antigen, effector T cell, medicine, Animals, Amino Acid Sequence, RNA, Messenger, 030304 developmental biology, DNA Primers, Base Sequence, Perforin, biology.organism_classification, Mice, Inbred C57BL, Immunization, CD8, 030215 immunology
Abstract

We generated T cell receptor transgenic mice specific for the liver stages of the rodent malaria parasite Plasmodium yoelii and studied the early events in the development of in vivo effector functions in antigen-specific CD8(+) T cells. Differently to activated/memory cells, naive CD8(+) T cells are not capable of exerting antiparasitic activity unless previously primed by parasite immunization. While naive cells need to differentiate before achieving effector status, the time required for this process is very short. Indeed, interferon (IFN)-gamma and perforin mRNA are detectable 24 h after immunization and IFN-gamma secretion and cytotoxic activity are detected ex vivo 24 and 48 h after immunization, respectively. In contrast, the proliferation of CD8(+) T cells begins after 24 h and an increase in the total number of antigen-specific cells is detected only after 48 h. Remarkably, a strong CD8(+) T cell-mediated inhibition of parasite development is observed in mice challenged with viable parasites only 24 h after immunization with attenuated parasites. These results indicate that differentiation of naive CD8(+) T cells does not begin only after extensive cell division, rather this process precedes or occurs simultaneously with proliferation.

Published
2001

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