1. A rapid real-time quantification in hybrid paper-polymer centrifugal optical devices.
- Author
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Kim, SeJin, Kim, Dami, and Kim, Sanghyo
- Subjects
- *
OPTICAL devices , *BIOMEDICAL engineering , *MICROFLUIDIC devices , *TRANSMISSOMETERS , *LIGHT absorbance , *LIGHT transmission - Abstract
Abstract The research progress in the centrifugal microfluidic platform provides great opportunities for simple and effective analytical measurements in a variety of areas including biomedical engineering. In this study, we propose an optical reader that can measure the transmittance in a very sensitive and rapid manner on a hybrid paper-polymer centrifugal disc platform. This device enables real-time monitoring of multiple samples by measuring the absorbance of the light transmitted through the paper integrated on the disc between the light emitting diode (LED) and the photodiode (PD) regardless of the ambient light condition. To confirm its efficiency, we analyzed one of the blood's important indicators, glucose in a successful manner within 10 s without any additional complex image analysis. In addition, we discussed the results by comparing with the reflectance-based methods and with those of the previously reported studies by introducing a figure of merit to evaluate the performance of the assay. Highlights • Optical reader was developed for hybrid paper-polymer centrifugal disc platform. • This device enables real-time detection by measuring light transmission through the paper integrated on the disc between LED and photodiode. • As proof of concept, enzymatic colorimetric glucose detection was successfully performed. • Micromolar detection was achieved using the present technique within 10 s without any additional complex image analysis. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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